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Summary

Proven ability with technological industrial skill sets

Chromatography and Mass Spectrometry for Proteomics application

Work experience

NOV 2018PRESENT

EXECUTIVE APPLICATION SUPPORT

Spinco Biotech pvt ltd
  • Application supports for Chromatography and Mass spectrometry.
  • Domain support for Proteomics and MALDI applications.
AUG 2017OCT 2018

Junior application specialist

Spinco Biotech pvt ltd
  • Application supports for Chromatography and Mass Spectrometry.

Skills

Mass spectrometry
  • MALDI-TOF Mass spectrometry (Shimadzu MALDI 7090, AXIMA Series).
  • LC-MALDI Proteomics (MuDPIT)
  • Mass Spectrometry Imaging.
Proteomics
  • Protein Digestion - Denovo peptide Sequencing , Peptide Mass Fingerprinting.
  • LC-MALDI Proteomics and Gel Proteomics.
  • Bottom up Proteomics.
  • Quantitative Proteomics.
Chromatography
  • Two - Dimensional Nano Liquid Chromatography coupled automated MALDI fraction spotter (Biomarker Identification).
  • Shimadzu HPLC - UV, PDA, Sedere-ELSD, Refractive Index (RI), Relative Fluorescence (RF).
  • SEC- MALS (Dynamic light Scattering -Wyatt  Devices -USA).
Softwares
  • MALDI solutions
  • AXIMA Launchpad
  • LC-MALDI
  • Mascot Database
  • Lab solutions
  • Nano assist
  • Accu-spot fraction spotter
  • Astra software
  • Bioinformatics 

Education

20152017

M.Sc.,Biotechnology

Alagappa university

University Rank Holder ; Distinction

20112014

B.Sc.,Microbiology

Muthayammal college of arts and science

University Rank Holder ; Distinction      

Training and visits

28th Jan 201930th Jan 2018

LC- MALDI Application Training

Kratos Analytical Ltd,Manchester, UK

LC - MALDI Application training for MALDI-7090 coupled with MuDPIT Technology

29th Oct 20182nd Nov 2018

1D Nano LC and Accuspot Service Training

Shimadzu (Asia Pacific) Pte Ltd, Singapore.

Service and Application training for Multi-Dimensional Nano Liquid Chromatography and Accuspot MALDI spotter system.

18th June 201822nd June 2018

MALDI-7090 Training with introduction to MALDI Imaging

Kratos Analytical Ltd

Training on  MALDI- 7090 Operation and Introduction to MSI

Projects

"The Role of immune pathways and mechanisms combating UV-A mediated stress response in model nematode Caenorhabditis elegans". 

My Project dissertation work is focused on understanding the key role of  MAP-Kinase pathways  p38 & JNK Map-kinase cascades in protecting the cellular system combating with immune response during  UV-A mediated stress condition.

  • Proteomics : Protein isolation & quantification, Western blotting, SDS-PAGE 
  • Genomics: Qualitative real time PCR
  • Caenorhabditis elegans as model organism.

" Analysis of C.elegans whole proteome during ageing process using short gun Proteomics approaches

  • Protein isolation & Quantification.
  • 2 Dimensional gel electrophoresis
  • Maldi Tof -Tof spectroscopy
  • Western-blot.

Accords

  • University 8th Rank  in Microbiology from Periyar university.
  • University 3rd Rank position in Biotechnology from Alagappa university.
  • Best youth red cross volunteer award received from Indian red cross society , chennai on 64th anniversary of Geneva conventions day(17th august 2013).

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