Teit Max Moscote Søgaard

Senior Scientist looking for a new challenge in major pharma

Summary

Having spent my career to date in R&D settings in academia and industry i am now looking to continue in a company that offers the potential to advance to a higher level.  My experience covers working at major research organisations abroad as well as in Biotech in Denmark and I am experienced both in cGMP process development and transfer to CRO as well as in writing and validation of methods for protein analysis/QC. 

Skills

Skills

Process development for cGMP production. Development of analytical methods. Process transfer and writing  of development reports and standard operating procedures.

Analysis of protein interactions by surface plasmon resonance, Iso thermal calorimetry and microscale thermophoresis.

Macromolecular protein purification by diverse modes of protein purification including IEX, HIC and mixed mode chromatography.

HPLC analysis of proteins by Size Exclusion-, Reverse Phase-, and ion-exchange-chromatography.

Preparation of samples for NMR analysis. set up of crystallisation screens. analysis of protein by SDS-PAGE, immunoblottting, ELISA, Gel flitration, differential scanning flourimetry.

Heterologous protein expression & purification (E.coli, yeast, insect cells, human cells)

Work History

Work History
Jul 2015 - Present

Senior Scientist

ExpreS2ion Biotechnologies

Project management, transfer of analytical methods to upscale CRO. Authoring of SOP for protein analysis. Development of analytical tools, writing development reports and transfer of these to CRO. Project management.

May 2013 - Jun 2014

Scientist

Expres2ion Biotechnologies

Responsible for the downstream development of a process for cGMP production of a malarial protein vaccine for a clinical phase 1 study and transfer of this process to CRO. 

Jun 2012 - Apr 2013

Research assistant

Department of structural Biology (STRUBI) University of Oxford

The Karlin group. High through-put cloning and expression of viral proteins for crystallisation. Purification and charcterisation of proteins and set-up of crystallisation screens

Nov 2006 - May 2012

Post doc

The Noble group, Department of Biochemistry, University of Oxford

Development of purification processes for proteins involved in cell focal adhesion and cell migration. Analysis of protein-protein interactions by diverse techniques. Set-up of crystallisation screens, preparation of samples for NMR analysis.

Jun 2006 - Oct 2006

Post doc

The Svejstup group, Cancer Research UK

Purification and characterisation of RNA polymease II from Yeast.

Aug 2001 - May 2006

PhD student

The Svejstrup group, Cancer Research UK

Basic research into transcription initiation in vitro using yeast as a model system. Characterisation of phosphorylation of the  C terminal repeat domain of RNA polymerase II and its consequences in basal transcription.

Jul 2000 - Jul 2001

Research Assistant

The justesen Group, University of Aaarhus

I developed an ELISA based assay for the quantification of translational read-through of stop codons in mamalian cells.

Education

Education
Aug 2001 - Jun 2006

Ph.D.

Enrolled at University College of London as a Cancer Research UK PhD student 

In the Svejstrup group I met the challenge of successfully conducting basic research in transcription in yeast. I greatly expanded my knowlegde of purification techniques and experienced the speed and efficiency of discovery in a world-leading research organisation.

Sep 1988 - Jun 2000

Cand. Scient. in molecular biology

Aarhus University

In the Justesen group I acquired a firm footing in molecular biology and protein chromatography. I worked with different groups both at the Aarhus university hospital and at the Department of Macromolecular Crystallography to solve very different types of problems

Aug 1985 - Jun 1988

Gymnasial afslutnings eksamen

Slagelse Gymnasium

Natural sciences

Publications

 

  •  Michael K. Lo, Teit Max Soegaard§, David G. Karlin, Evolution and structural organization of the C proteins of Paramyxovirinae (§Denotes equal contribution), PloS one, 9(2), p.e90003. Jan 2014 
  • Max M. Søgaard and Jesper Q. Svejstrup Hyper-phosphorylation of the carboxyterminal repeat domain of RNA polymerase II facilitates dissociation of its complex with mediator J Biol Chem. 2007 May 11;282(19):14113-20
  • Somesh BP, Reid J, Liu WF, Sogaard TM, Erdjument-Bromage H, Tempst P, Svejstrup JQ. Multiple mechanisms confining RNA polymerase II ubiquitylation to polymerases undergoing transcriptional arrest. Cell. 2005 Jun 17;121(6):913-23.
  • Petrakis TG, Sogaard TM, Erdjument-Bromage H, Tempst P, Svejstrup JQ. Physical and functional interaction between Elongator and the chromatin associated Kti12 protein. J Biol Chem. 2005 May 20;280(20):19454-60.
  • Kong SE, Kobor MS, Krogan NJ, Somesh BP, Sogaard TM, Greenblatt JF, Svejstrup JQ. Interaction of Fcp1 phosphatase with elongating RNA polymerase II holoenzyme, enzymatic mechanism of action, and genetic interaction with elongator. J Biol Chem. 2005 Feb 11;280(6):4299-306.
  • Smidt KC, Hansen LL, Sogaard TM, Petersen LK, Knudsen UB, Martensen PM.A nine-nucleotide deletion and splice variation in the coding region of the interferon induced ISG12 gene. Biochim Biophys Acta. 2003 Jul 30;1638(3):227-34.
  • M. Martensen, T. M. M. Søgaard, I. M. Gjermandsen, H. N. Larsen, A. Bejder, V. Bonnevie-Nielsen, C. Rosada, J. L. Simonsen, and J. Justesen The interferon alpha induced protein ISG-12 is localized to the nuclear membrane. Eur J Biochem. 2001 Nov;268(22):5947-54.
  • Charlotte G. Jakobsen, Teit Max M. Søgaard, Olivier Jean-Jean, Lyudmila Yu. Frolova and Just Justesen Identification of eRF3b, a human polypeptide chain release factor with activity in vitro and in vivo as eRF3. Mol Biol (Mosk). 2001 Jul-Aug;35(4):672-81
  • René Jørgensen, Max M. Søgaard, Anette Bejder, Pia M. Martensen and Just Justesen,  Identification and characterization of the human mitochondrial tryptophanyl-tRNA synthetase. J Biol Chem. 2000 Jun 2;275(22):16820-6.
  • M. M. Søgaard, Charlotte G. Jacobsen, Just Justesen A sensitive assay of translational fidelity (readthrough and termination) in eukaryotic cells. Biochemistry (Mosc). 1999 Dec;64(12):1408-17.
  • Pedersen B, Andersen CL, Søgaard T.M.M, Nørgaard JM, Koch J, Krejci K, Brandsborg M, Clausen N Trisomy 10 survival: a literature review and presentation of seven new cases. Cancer Genet Cytogenet 1998 Jun 103:2 130-2