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Surya Aathmanathan

Applying for Ph.D. Programme




Research Scholar

Under the guidance of M.Krishnan, Professor, Insect Molecular Biology unit, Department of Environmental Biotechnology,Bharathidasan University, Tiruchirappalli (India)


M.Sc. Genomics

Madurai Kamaraj University, Madurai (India)



B.Tech Biotechnology

Anna University, Chennai (India)



Higher Secondary Certificate

Vidhya Vikas Boys High School, Thiruchengode (India)



Secondary School Leaving Certificate

TVS Matriculation Higher Secondary School, Madurai (India)


Techniques Learnt

Bacterial Genetics Techniques: Plaque assay, Lambda lysogen induction, in-vivo cloning, operon induction, β-gal assay, auxotroph isolation, ,Triparental Mating.

Horizontal Gene Transfer Techniques: Transduction, Transformation  and Conjugation.

PCR based techniques: RT-PCR, Semi quantitative PCR ,Colony PCR and PCR based mutagenesis.

Electrophoretic techniques: PFGE, DGGE, AGE, PAGE.

Genomics techniques: Whole Genome Sequencing, Contig assembly, functional gene prediction.

Gene Cloning & Expression: Genomic and Plasmid DNA isolation, RNA isolation, c-DNA synthesis,Restriction Digestion, Cloning, Blue/White Colony Selection, Protein Expression, Purification (Affinity Chromatography) and Quantification  Protein-Protein Interaction via Yeast Two Hybrid Analysis.

Mutational Studies: Physical, Chemical and Biological Mutagenesis in E.coli.

Transfection: Liposome mediated siRNA delivery.

Neurophysiology : Extracellular recordings in the inferior colliculus of mouse and Intracellular recordings from neurons in leech ganglion using glass electrodes

Immunological Techniques: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, lympho cytotoxicity assays, immunodiffusion

Flow Cytometry: Cell cycle, apoptosis and mitochondrial biomass analysis,High Content Screening (Apoptotic Studies)

Bioinformatics: Homology modelling, Protein-Protein Interaction, Molecular Docking, Molecular Dynamic Simulation.

Language Skills

LanguageCan WriteCan Speak Can read

Research Experience:

Since my childhood days I have developed a keen interest in science. This passion made me to set my career in science. My research experience started during the third year of my Bachelor’s programme. My first work was on Bioinformatics titled "Screening of antiviral phytochemical compounds against Dengue NS3 protese" which involved screening of 300 ligand molecules with a crucial druggable candidate of NS3 protease of Dengue Virus. The ligands were downloaded from the chemspider database and NS3 protease was predicted using Modeller. The homology model was validated by Ramachandran plot and dynamic simulation.  After validation the 300 ligands were screened against NS3 protease using PyRX software. I got a potential hit for Hypercin dicarboxylic acid with binding affinity -16.34 kcal/mol. Then during my Bachelor's dissertation work I proposed a project entitled "Eco-friendly Biodegradable Films from Aloe vera" to Tamil Nadu State Council for Science and Technology (TNSCST), Government of India, for which I was sanctioned an amount of Rs.8000 INR from the funding agency. The Biodegradable films were prepared using Poly vinyl alchol (PVA) as an additive. Different proportions of Aloe vera extracts were used to cast polymer films.The films were analysed for thermal, tensile strength and biodegradable properties. The optimum proportion which had good thermal, tensile strength and biodegradable properties was found to be 60:40 of PVA: Aloe vera extract respectively. During my M.Sc. Genomics programme at School of Biological Sciences, Madurai Kamaraj University, Madurai (India), I was fascinated on bacterial genetics which inspired me to join the group headed by Dr.M.Hussain Munavar, Professor and Head, Department of Molecular Biology, School of Biological Sciences, Madurai Kamaraj University, Madurai (India) for my M.Sc. Dissertation work. I started my work under the guidance of Dr.B.Singaravelan on the work entitled "Isolation and Partial Genetic Characterization of null dnaQ - Δlon Incompatibility Suppressors (dls) in Escherichia coli". Synthetic lethality is an emerging concept in the field of genetics. Currently works are being carried out worldwide to use this concept even in treating cancer. Synthetic lethality arises when mutations in two different genes which cannot co-exist in a bacteria which is known as genetic incompatibility. Previous workers in our lab had isolated suppresors for null dnaQ - Δlon incompatibility and named them as dls14 and dls20. I was assigned the task of characterizing the suppressors. We designed the experiments to map the mutations using elegant transductional crosses. Interestingly for dls20 the mutation mapped near the region of umuDC which is error prone polymerase involved in Bacterial SOS response. Further to confirm the mutation in umuDC region was sequence analysed twice. A transition mutation of A831 – G831, leading to Lysine to Gluamic acid amino acid change was noted. Similarly through transductional crosses the mutation in dls14 was mapped in the region of dnaJ, coding for a molecular Co-Chaperone. Further characterization is being carried out by others in the laboratory. My dream always was to pursue Ph.D. in abroad. Soon after M.Sc. I got an opportunity and joined the pioneering Insect Molecular Biology unit of Dr.M.Krishnan, Professor, Department of Environmental Biotechnolgy, Bharathidasan University, Tiruchirapalli (India). Here I am working on topic entitled "Characterization of Hemolin from silk worm Bombyx mori: As a carrier protein in vaccine development". I am in the initial stages of my work.

Papers under communication:

M. Kannan, V. Surya Aathmanathan, Meenakshi Saravanakumar, Abdul Jaleel, Davide Romanelli, Gianluca Tettamanti, M. Krishnan (2016). Proteomic analysis of silkworm during larval to pupal transition of Bombyx mori. Submitted to Invertebrate Survival Journal (IF: 1.02).

Conferences Attended/Paper Presented

  • Attended  "World Immunology Day Conference" held at  School of Biotechnology , Madurai Kamaraj University on April, 2015. 
  • Attended  " National Science Day Conference on Modern Biology" held at  School of Biological Sciences, Madurai Kamaraj University on February, 2015
  • Attended "Biotechellence – AnnualNationalConference,'14" held at Anna University,Guindy campus, Chennai on March, 2014.
  • Attended the "Academies Lecture Workshop On Recent Research In Biological Sciences,National Science Day Conference" held at Madurai Kamaraj University February, 2014. 
  • Presented a paper in "International Conference of Bioinformatics and Systems Biology" held at Annamalai University, Chidhambaram on Feburary, 2012


  • Cleared all India level DBT-IPLS exam 
  • Awardee of DBT-IPLS Scholarship
  • Awardee of " Student Project Scheme Fund" by Tamil Nadu State Council for Science and Technology (TNSCST), Goverment of India.

Digital Competence

  • Good command in Microsoft Office.
  • Amateur in Photo editing tools.
  • Basic Knowledge in operating Image J tool.


Dr.M.Hussain Munavar,                           

Professor and Head,

Department of Molecular Biology,

School of Biological Sciences,

Madurai Kamaraj University,

Madurai, Tamil Nadu,

India - 625021.

Mail id:  , Phone: (+91) 9486230021




School of Environmental Sciences,


Department of Environmental Biotechnology,

Bharathidasan University,

Tiruchirappalli, Tamil Nadu,

India – 620024.

Mail id:, Phone: (+91) 9443998251



Associate Professor and Head,

Department of Genetics,

School of Biological Sciences,

Madurai Kamaraj University,

Madurai, Tamil Nadu,

India - 625021

Mail, Phone: (+91) 9443896541






I declare that the information given above is true to the best of my knowledge and belief.


Date: 04/05/2016.                                                                                                               Yours Sincerely,             Place: Tiruchirappalli (India)                                                                                        V.Surya Aathmanathan