Molecular Biology Techniques: DNA, RNA and protein Purification, PCR, Sanger sequencing, western blotting, Real time PCR, molecular cloning.
Cell culture Techniques: Primary neuronal cultures, cerebellar cultures, organotypic slice cultures, microdissection, whole brain cultures, ex-utero electroporation, microcontact printing for cell culture, FAC - sorting, experience in cell preperation for C1-Fluidigm chip, cell cycle analysis and time lapse imaging.
Histology Techniques: Cryosectioning, vibratome sectioning, microtome sectioning, Immunohistochemisty (DAB and Fluorescence), fluorescence, confocal and light sheet imaging, perfusion, DiI implantation in fixed brains
In vivo Techniques:In utero thalamic and cortical electroporation, Flash Tag, stereotactic injections, transplantation of cells in-vivo, c-row barrel deprivation, untrasound guided injection of mice pups, animal handling, retrograde labelling using DiI and CTB.
Informatics: Illustrator, Photoshop, Prism, Basics coding skills in Matlab, R, C, C++ and Unix.
Bioinformatics: RNA sequencing analysis (single cell and bulk), Gene enrichment analysis (SCDE), cell clustering and lineage analysis(Pagoda, Waterfall and Monocle package), Gene ontology enrichment analysis. Trained in ChiP seq analysis and transcriptomic analysis using Minion sequencer.