Molecular Biology Techniques: DNA, RNA and protein Purification, PCR, Sanger sequencing, western blotting, Real time PCR, molecular cloning.
Cell culture Techniques:Primary neuronal cultures, cerebellar cultures, organotypic slice cultures, microdissection, whole brain cultures, ex-utero electroporation, microcontact printing for cell culture, FAC - sorting, experience in cell preperation for C1-Fluidigm chip, cell cycle analysis and time lapse imaging.
Histology Techniques: Cryosectioning, vibratome sectioning, microtome sectioning, Immunohistochemisty (DAB and Fluorescence), fluorescence, confocal and light sheet imaging, perfusion, DiI implantation in fixed brains
In vivo Techniques:In utero thalamic and cortical electroporation, Flash Tag, stereotactic injections, transplantation of cells in-vivo, c-row barrel deprivation, untrasound guided injection of mice pups, animal handling, retrograde labelling using DiI and CTB.
Informatics: Illustrator, Photoshop, Prism, Basics coding skills in Matlab, R, C, C++ and Unix.
Bioinformatics: RNA sequencing analysis (single cell and bulk), Gene enrichment analysis (SCDE), cell clustering and lineage analysis(Pagoda, Waterfall and Monocle package), Gene ontology enrichment analysis. Trained in ChiP seq analysis.