- Children's National Medical Center Washington DC
- [email protected]
2005-2011: PhD Biotechnology University of Pune, India, 2003-2005: M.Sc. Industrial Microbiol. Devi Ahilya Vishwa Vidyalaya, India.
2002-2003: P.G.dip Mol. Biol. and Biochem. Tech. University of Delhi, India,
1999-2002: B.Sc.Microbiology (H) University of Delhi, India, .
ELISA, ELISPOT, flow cytometry-BDCalibur, BDCanto, BDAria for cell sorting and BDVerse for high throughput assays, surface and intracellular staining.
Isolation of primary cells (T and B-lymphocytes) lymphocyte isolation from human blood and tissue samples, handling infectious HIV and herpesviruses in BSL2 facility.
Designing lentiviral constructs and production of viral particles including infectious HIV
RNA extraction, cDNA synthesis, Real-Time PCR-TaqMan chemistry, PCR arrays, differential gene expression profiling, gene copy number analysis, western blotting, transfection, nucleofection and stable clone production.
Handling of Mycobacterium-infected guinea pigs and organ extraction for histology in BSL3 facility.