Sibabrata Mukherjee

Scientist at Novozymes

Accomplishments

Work History
Apr 2015 - Present

Scientist, R&D-Application Technology

Novozymes South Asia Pvt. Ltd Bangalore, India.

Application Development of Industrial Enzymes in Food and Non-Food Sectors by leveraging enzyme molecules across different business units and selecting the best enzyme/s for specific applications

Overview of Work flow:

  • Scouting of Potential Application Scope for Industrial Enzymes
  • Scoping of Project with Various Stake Holders viz. Business operations, Technical Services, Regulatory, IP
  • Bench Mark setting for different Gating systems with strategic steering committee
  • Short listing of on shelf product based on the biochemical characeteristics
  • Development of SOP for performance analysis of the enzyme simulating industrial conditions in the lab
  • Screening of Enzymes and generation of lab scale trial data
  • Getting input on Freedom to operate and sustainability
  • Pilot scale/Large scale trial with selected enzymes
  • Input giving to Protein Engineering or Formulation team for further improvement of the molecule/formulation based on the trial results
  • Secure/Process IP on novel findings (Process and Product)
  • Transfer Technology to Technical Services Team and BO after demonstration of successful Plant Trial

 

Designing of Lab Experiments on daily basis and leading Lab Team of four Research Associates, one Associate Scientist and Four Interns to generate data.

Handling various Carbohydrases, Proteases and Lipases for various goals viz. Biomass Hydrolysis by Cell Wall Degradation.

Analyzing the data statistically and Present to Internal and External Higher-Ups.

Scaling up of Lab technology to Plant Scale and formulating SOPs for Technology Transfer.

Leading Plant Scale Trials along with Technical Services People and Closing Mass balance in Customer/Partner’s place.

Liaisoning with Technical Services Team and Business Operation team for understanding challenges, formulating mitigation plan and alignment.

Present research work in scientific forum and International Conference.

Filed 3 nos of IPs on the Research outcomes.

Aug 2012 - Mar 2015

Sr. Executive Biotech Department, Chemical Division

THERMAX LTD. Pune, India.

Developed new Lab Setup for Industrial Biotech Research from the scratch.

Trained people for Lab activities.

Regular Project Up date to Higher-ups with Techno-Commercial Presentation.

Analysed Technical Enzyme Market and promoted Ideas to Kick off New Projects matching with Company Portfolio.

Lade Projects to develop Enzyme formulations for Pulp and Paper, Biomass Hydrolysis Applications.

Technology Transferred to Production Team and Lead Commercial Production of Enzyme Formulation.

Developed full range of Enzyme portfolio for Pulp and Paper: De-sizing, Pitch Control, Refining, De-inking and Pulp Bleaching-All are commercialized by the BO Team.

Liaisoned with Govt. Research Institutes for fermentation based product development: Formulation of MOU, Bench mark setting of the final goods and Kick off projects – Production of In-house enzymes viz. Cellulase, Amylase and Xylanase; Lactic Acid and Poly Glutamic Acid.

Developed Bench scale fermentation and Down Streaming processes for Enzymes and EM adopting Statistical Optimization Approach.

Developed Effective Microbial Consortia (EM)-Powder Product in Lab and lade Commercial Production in large scale Fermentation set up aided with Spray Drying facility.

Established Plant Scale Application of In-house developed EM formulation for Waste water Treatment.

Sep 2011 - Aug 2012

Sr. Research Technologist

PRAJ-Matrix: R&D Centre PRAJ Ind. Ltd. Pune, India.

Engaged in Enzyme Application for Biomass Hydrolysis for 2nd Gen Ethanol Production.

Involved in Design of experiments, execution, data analysis and presentation.

Did Dose response and process parameter optimization of enzymatic hysrolysis for maximize C6 Sugar yield from Pre-treated Biomass (Corn cobs, Sugar cane bagasse, corn stover) in flask, bench and pilot scale.

Established Surfactant aided enzymatic process with superior hydrolysis and sugar yield.

Developed detoxification process using Ion exchange resin and polyelectrolyte applications for C5 stream generated during pre-treatment for better fermentability by the selected microbes.

Hands-on lab-scale fermentation including independently setting up and operating a fermenter and troubleshooting scenarios that arise during fermentation [Scale: flask, 5L, 20L, 100L].

Designing and performing biochemical assays of enzymes; utilizing HPLC methods, Spectrophotometer, electrophoresis and basic biotechnology lab equipment to analyze samples from lab and industrial pilot scale experiments.

Performing compositional analyses of biomass using FT-IR, HPLC to assist in development of cellulosic biofuel technologies;

Performing statistical analysis of data and working with aseptic cell culture and microbiology techniques.

Anaerobic single pot fermentation of lignocellulosic substrate to ethanol using proprietary microorganism.

Associated for the development of membrane based enzyme recovery and reuse (ERR) model for the production of lignocellulosic hydrolyzate.

Jun 2011 - Sep 2011

Manager QA & ISO Coordinator

SAJ Food Ind Pvt Ltd (BISKFARM), Kolkata, India.

Take care of entire QA system and management of the bakery products.

Supervision of Microbiological and chemical QC analysis of bakery products.

Entire supervision and troubleshoot to the ETP.

Designing of activated sludge process from lab scale to plant scale.

ISO22000 documentation planning, review and implementation.

2007 - 2011

Research Fellow

University: JADAVPUR UNIVERSITY
RESEARCH FELLOW Industrial project sponsored by IFB Agro Ind Ltd. Production, isolation, purification of industrially important enzymes for starch, cellulose and hemicelluloses hydrolysis to sugar for alcohol fermentation. Hands on experience in large scale starch based ethanol production[Scale: 1000 KL].

Education

Education
Sep 2007 - May 2011

PhD

Jadavpur University, Dept. of Food Technology & Bio-Chemical Engineering

Field of Research: Enzyme Technology & Microbial Fermentation

Thesis Title: Studies on the production of carbohydrase enzymes by Aspergillus oryzae on solid substrate. Index No. 235/09/E

May’11 Joined Industrial Job after completion of Research Work and Thesis Submitted on Oct'15

Projects Handled during PhD as Research Fellow

  1. Production, isolation, purification of industrially important enzymes for starch, cellulose and hemicelluloses hydrolysis to sugar for alcohol fermentation. Sponsored by IFB Agro Ind. Ltd. India.
  2. Survey on The Heavy Metal Contamination in Fish Collected from River Ganges, Lakes and other Rivers in West Bengal. Sponsored by University Grunt Commission India.
  3. Survey on The Heavy Metal Contamination in Fruits and Vegetables in West Bengal. Sponsored by Dept. of Food Processing and Horticulture, Gov. of West Bengal
Aug 2005 - Aug 2007

M.Sc. Microbiology

UNIVERSITY OF CALCUTTA

1st Class, Regn. no. 020647,  2002-03

Aug 2002 - Jul 2005

B.Sc (Hon) Microbiology

UNIVERSITY OF CALCUTTA

1st Class, Regn. no. 020647,  2002-03

Skills

Skills
8
  • Development of strategic business plan for the Microbiology and Biotech department.
  • Preparation of annual business plan for the Biotech department which includes the milestones, budget for the capex, G&A and the man power expenditure as well.
  • Market analysis of the biotech based product which can be chanalized through the existing business line viz. performance product group, pulp and paper chemicals, ion exchange resin group, construction chemical group, oil field chemical groups and specialty group. 
  • Selection of the product lines and prioritization of the projects for the selected development. Negotiation with the top management regarding the project evaluation.
  • Liaisoning with research institute and the specialized industries for the new product and business development.   
  • Project management. Technology integration in business terms. Customer focused product development.
  • Bioprocess development. Enzymatic hydrolysis of starchy and cellulosic biomass.
  • Fermentation development for the production of alcohol, organic acids and biopolymers.
  • Over all laboratory management.
  • Use of statistical tools for optimization.
  • Technical presentation for business development.

PATENT & PUBLICATIONS 

  • Indian patent applied: METHOD FOR EFFICIENT ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS.
  • A study on the effects of environmental factors on fungal α-amylase synthesis utilizing cereal processing mills’ residues. Sibabrata Mukherjee1*, Sujata Kulkarni2 and Milind Kulkarni. International Journal of Application or Innovation in Engineering & Management (IJAIEM) in Jan’14. 2014.
  • Optimization of the Physical Process Parameters for the Endo-Glucanase Production by Aspergillus Terreus Grown on Soy Hull Based Substrate Through Solid State ermentation. Sibabrata Mukherjee*, Sujata Kulkarni and Milind Kulkarni. International Journal of Engineering Research & Technology (IJERT) Vol. 3 Issue 2, February – 2014.
  • RECOVERY OF ASPERGILLUS ENDO-GLUCANASE PRODUCED ON SOLID SUBSTRATE: A DOE BASED APPROACH. Sibabrata Mukherjee*, Sujata Kulkarni, Milind Kulkarni . JMBFS. 2014.
  • Covalent immobilization of amyloglucosidase on Tulsion® weak base anionic resin: optimization by Box-Behnken DOE followed by thermal characterization. Sibabrata Mukherjee , Milind Kulkarni, Sujata Kulkarni, Shirish Naik. The Journal of Bioprocess Technology. 2013.
  • HEAVY METAL CONTAMINATION IN FRUITS AND VEGETABLES IN TWO DISTRICTS OF WEST BENGAL, INDIA Debopam Banerjee, HimadriBairagi, SibabrataMukhopadhyay, Anju Pal, DebabrataBera, Lalitagauri Ray* Department of Food.Tech& Biochemical Engineering, Jadavpur University, Kolkata – 700032, India EJEAFChe, 9 (9), 2010. [1423-1432].
  • HEAVY METAL CONTAMINATION IN VEGETABLES COLLECTED FROM MARKET SITES OF KOLKATA, INDIA. Banerjee, D., Kuila, P.; Ganguli, A.; Das, D.; Mukherjee, S.; Ray, L.*, Department of Food Technology and Biochemical Engineering, Jadavpur University, Kolkata – 700032, India EJEAFChe, 10 (4), 2011. [2160-2165].

ABSTRACTS & MEETINGS

  • In 2015, Presented Poster on Research work at Novozyme, PIPOC'15
  • In 2013, Presentation on Green chemicals through white biotech in ICABB’14, Pune.
  • In 2013, Corporate Paper presentation on Biotech initiatives for Thermax Ltd. Nov’13 in International Conference on Advances in Biotechnology & Bioinformatics & X Convention of The Biotech Research Society,India 25th to 27th November 2013.
  • In 2013, presented poster on the Biotech development in Thermax during 5th India-Korea Joint Workshop on Bioenergy Incorporating Biofuels and Biorefineries held at September 9-10, 2013 at CSIR-National Institute for Interdisciplinary Science and Technology, Trivandrum.
  • In 2013, Presented poster in the in the Technology Day on Biotech product development at Thermax Ltd.
  • Hydrolytic cocktails from Aspergillus oryzae using crop processing mills residues. Sibabrata Mukherjee, SubhabrataSengupta, Lalitagauri Ray. Ready to be communicated.
  • Development of a mutant Aspergillus oryzae producing raw starch hydrolyzing amylase. Sibabrata Mukherjee, SubhabrataSengupta, Lalitagauri Ray. Ready to be communicated.
  • In 2010, presented a paper with the abstract entitled STUDIES ON THE THERMODYNAMICS AND HEAT INACTIVATION KINETICS OF AMYLOLYTIC PREPARATION FROM Aspergillus oryzae JU9 in International Conference on “Biotechnology for Better Tomorrow” (BTBT-2011), Organized byThe DepartmentfMicrobiologyDr.BabasahebAmbedkarMarathwadaUniversity, Aurangabad, Maharashtra, (INDIA).
  • In 2010, presented a paper entitled “Hydrolysis of raw starch by the enzyme composition obtained by solid state fermentation by Aspergillus sp JU9 on agro industrial waste” in International Conference On Biotechnology, Food and Bioengineering -Emerging Trends And Future Prospects ICBFBE – 2010 organized by CENTER FOR RESEARCH AND DEVELOPMENT, PRIST UNIVERSITY Thanjavur and INDIAN INSTITUTE OF CROP PROCESSING TECHNOLOGY (IICPT) (GOI). Thanjavur, Tamilnadu.
  • In 2009, presented a poster with the abstract entitled “Production of amylolytic enzymes from Aspergillus oryzae JU9 in solid state fermentation and development of an ideal starch degradation process” in a National Conference named XX Indian Convention of Food Scientists and Technologists (ICFOST) 2009 organized by Association of Food Scientists &.Technologists (India), Mysore [AFSTI] in association with CFTRI, Mysore, DFRL, Mysore and NDRI, Bangalore held at NIMHANS, Bangalore, India.
  • In 2010, presented a paper with the abstract entitled “Endo xylanase production by Aspergillus oryzae JU9 via solid state cultivation (SSC) on lignocellulosic residues and its physicochemical characterization” in
  • InternationalWaterAssociation-IWA’s specializedconferenceon“MicrobesinWastewater&WasteTreatment,BioremediationandEnergyProduction” orgtanized by The Birla Institute of Technology and Science (BITS) – Pilani, Goa campus, India and Institute National de la RechercheAgronomique(INRA),France.
  • In 2009, presented a poster with the abstract entitled “STUDIES ON THE EFFECTIVENESS OF Cu (II) ION DESORPTION WITH ACIDIC, ALKALINE AND CHELATING REAGENTS FROM THE IMMOBILIZED BIOMASS OF Bacillus cereus M116” in a National Conference named “Future of food biotechnology in India” held at National Institute of Technology Durgapur, India.
  • In 2006, presented a poster with the abstract entitled “MYCOPROTEIN THE MICROBIAL MEAT SUBSTITUTE” in an UGC sponsored state level symposium at BarrackporeRastroguruSurendranath College, West Bengal, India.