Shilpi Das

  • Jamshedpur  Jharkhand
Shilpi Das

Research Trainee

Work History

Work History
Mar 2015 - Jul 2015

Research Trainee

CromDx Solution Private Limited,Noida,U.P.

Project -“ Development of a molecular genetic testing method for Abacavir in Indian population”.

CromDx is unique company bringing Clinician, Physicians and Research community to common platform as stakeholders in advance clinical care requirements of modern era. It is fundamentally a molecular information platform which integrates molecular biology Lab and Clinical/Biological informatics together to develop and launch population specific diagnostics test and prognostics assessments. The project deals with the Development of a molecular genetic testing method for Abacavir in Indian population. Abacavir is a prescription medicine approved by the U.S. Food and Drug Administration(FDA) for the treatment of HIV infection in adults and children 3 months of age and older. Abacavir is always used in combination with other HIV medicines. Abacavir belongs to a class(group) of HIV drugs called nucleoside reverse transcriptase inhibitors(NRTIs). NRTIs block an HIV enzyme called reverse transcriptase. By blocking reverse transcriptase, NRTIs prevent HIV from multiplying and can reduce the amount of HIV in the body. HIV medicines can't cure HIV/AIDS, but taking a combination of HIV medicines(called an HIV treatment regimen) every day helps people with HIV live longer, healthier lives. HIV medicines also reduce the risk of HIV transmission. Individuals with the HLA-B* 5701 allele is found to be more likely to have a hypersensitivity reaction to abacavir. The HLA-B* 5701 allele occurs at a frequency of around five cert in European populations, one pent cent in Asian populations and less than one per cent in African populations. Clinical trials have now shown that screening patients for HLA-B* 5701 before treatment has dramatically reduced the number of side effects being experienced from abacavir use. Abacavir can cause serious, life threatening side effects. These include allergicreactions, lactic acidosis(buildup of acid in the blood), and liver problems. If the individuals found to have the HLA-B* 5701 allele, abacavir is avoided, and alternative HIV treatments are given. The same topic is studied by taking samples(buccal swab) from few people and its DNA was extracted followed by PCR optimization and Sequencing

Jul 2014 - Oct 2014

Project Trainee

 KIIT School of Biotechnology, Bhubaneswar

 Project -  Bioremediation of organophosphorus pesticide, chlorpyrifos by soil isolates.

Chlorpyrifos is a crystalline organophosphate toxic insecticide, used in agriculture primarily on rice, cotton, oranges, corn, and among many other crops, it act as an inhibitor to acetylcholine esterase pathway which directly can damage nervous system. And the study focuses on to see the diversity of microbial load in soil contaminated with chlorpyrifos and check its degradation activity. Key Learnings ? Isolation and screening-Samples isolated from soil are screened for chlorpyrifos degradation in a medium plate. Growth curve analysis of microbial isolates in different carbon source. Standardization of media.

May 2013 - Dec 2013

Summer Trainee

 KIIT School of Biotechnology, Bhubaneswar

Project - Production of biodiesel from waste cooking oil and study its feasibility and process optimization.

Biodiesel, a clean renewable energy has drawn attention to the world at large due to its non-toxicity, biodegradability and better efficiency than non-food crops like Jatropha, where expensive process optimization keeps the product cost high. The concept relies on simple green chemistry clubbed to a techno-economically feasible strategy to produce biodiesel from waste fatty acids and organic waste(waste cooking oil) with a commercially and ecologically sustainable approach. Its application includes kerosene stove using biodiesel blends, biodiesel lantern and biodiesel bhatti for cooking purpose.

Jun 2012 - Jun 2012

Summer Trainee

Tata Main Hospital, Jamshedpur , Jharkhand                                          

Project -  To test different pathological samples and infer its causative agents and related diseases.

Tata Main Hospital, the Medical Division of Tata Steel, which is more than a century old, provides patient treatment with specialized staffs and equipments. The aim of  the internship was testing different pathology samples like urine and sputum by using staining techniques and its causative agent and disease was identified and characterized.


Sep 2010 - Jul 2015

Dual Degree (B.Tech- M.Tech) in Biotechnology

KIIT University, Bhubaneswar

Done Dual Degree in Biotechnology with Environmental Technology as Elective subject from KIIT University, Bhubaneswar, Odisha, India.

Jun 2008 - Jun 2010


Kerala Public School, Kadma (C.I.C.S.E)

Done my intermediate from Kerala Public School, Kadma, Jamshedpur, Jharkhand.




Environmental Technology

Bacterial taxonomic classification -DNA extraction, quantification by nano drop method, spectro- photometer and gel electrophoresis, primer designing, PCR, 16srRNA sequence, Phylogenetic tree analysis. Metagenomics DNA analysis - DNA isolation.Chemical analysis, physical analysis and biological analysis of water sample, MPN method, phage assay,enzyme assay. ARDRA of the PCR amplified 16S rRNA using tetracutter enzymes. Conversion of waste oil by transesterification to Biodiesel and to estimate its quality. Use of alginates for enzyme immobilization.

Chromatographic techniques

TLC, affinity chromatography, precipitation of proteins, 2D TLC, gel filtration chromatography.

Molecular biology

Isolation of plasmid and genomic DNA from E. coli, Isolation of DNA from plant and chicken liver, gel electrophoresis, SDS page, silver staining, cloning technique, isolation of RNA from cell lines and chicken cell

Immunological techniques

Calculation of percentage of hemolysis of RBC, Ouchterlony double diffusion, isolation of lymphocytes using sucrose gradient centrifugation, ABO blood grouping system, macrophage infection Assay.

Basic Microbiology techniques

Basis of bacterial culture, characterization and isolation of bacterial culture, its growth curve and pattern, antibiotic sensitivity test, staining techniques, soil, water and air sampling, testing for food spoilage