Sébastien Vigneau

  • Philadelphia US-PA

Work History

Work History
Oct 2003 - Sep 2007

Graduate student

Pasteur Institute

Functional study of the X-chromosome inactivation process using targeted deletions within the mouse X-chromosome inactivation centersupervised by Dr. Philippe ClercMouse Molecular Genetics unit led by Pr. Philip AvnerPasteur Institute, Paris, France.

Sep 2002 - Sep 2003

Undergraduate student

Ecole Normale Supérieure

Characterization of Dbx1 expressing cells identity and migration in mouse telencephalonsupervised by Dr. Alessandra PieraniBrain Regionalization team led by Dr. Marion WassefEcole Normale Supérieure, Paris, France.

Jul 2001 - Aug 2001

Undergraduate student

Biosentec

Developing an ethanol enzymatic biosensorBiosentec inc., Toulouse, France.

Feb 2001 - Jun 2001

Undergraduate student

Institut National de Recherche Agronomique

Developing a functional complementation system in Medicago truncatula with the aimof performing positional cloning of the DMI1 genesupervised by Dr. Jean-Michel AnéSymbiotic signals team led by Jean DénariéInstitut National de Recherche Agronomique, Toulouse, France.

Jul 2000 - Aug 2000

Undergraduate student

CerCo-CNRS

Modeling vision evoked potentials obtained by electroencephalogramssupervised by Margot TaylorMargot Taylor’s teamCerCo-CNRS, Toulouse, France.

Education

Education
Oct 2003 - Sep 2007

PhD

Pierre and Marie Curie University

Functional study of the X-chromosome inactivation process using targeted deletions within the mouse X-chromosome inactivation center.PhD obtained the 19th of September 2007supervised by Dr. Philippe ClercMouse Molecular Genetics unit led by Pr. Philip AvnerPasteur Institute, Paris, France.

Sep 2002 - Sep 2003

Master of Science (Diplôme d’Etudes Approfondies)

Pierre and Marie Curie University

Practical Courses of Embryology (mouse, chicken, frog, zebrafish, drosophila, c. elegans, starfish andsea-urchin)Seminars on Embryonic Polarity, Organizers and Inductions in Embryos, Mouse Development,Myogenesis, Chromatin, EvoDevo, New Methodologies in Developmental Biology.

Jan 1998 - Sep 2002

Bachelor of Science (Maîtrise)

Paul Sabatier University

Cellular and Molecular Biology, Genetics, Developmental Biology, Neurobiology, Bioinformatics.

Other Experiences

Deployment  of a groupware and project management software for the Penn Biotech Group

Fall 2009

Entrepreneurship project with the Penn Biotech Group

Fall 2009

Market study for a behavioral therapy software

Consulting project with the Penn Biotech Group

Spring 2009

Market study for a microarray analysis software

Webmaster for the Penn Biomedical Postdoctoral Council

Present

Currently redesigning the association website, new website to be released soon

Elected member at the Council of Studies and Student Life of the University Paul Sabatier (Toulouse, France)

2002-2003

Member of the finance commission

Elected member at the Biology Department Council of the University Paul Sabatier (Toulouse, France)

2001-2003

Elected member at the Undergraduate ("DEUG") Council of the University Paul Sabatier (Toulouse, France)

1999-2001

Courses and Meetings

Emerging Evidence For Epigenomics Changes In Human Disease Meeting

March 16-17th 2009

NIH campus, Bethesda, MD, USA

67th Society for Developmental Biology Annual Meeting

July 26-30th 2009

Philadelphia, PA, USA

6th International Society for Stem Cell Research Annual MeetingJune 11-14th 2008Philadelphia, PA, USA

Mouse Molecular Genetics MeetingSeptember 4-9th 2007Hinxton, Cambridge, UK(Poster presentation)

Journées Boris EphrussiJune 7-8th, 2007Forum des Cordeliers, Paris, France(Poster presentation)

Scientific Meeting of the Departement of Developmental Biology of the Pasteur InstituteOctober 18-19th, 2006Institut Pasteur, Paris, France(Oral communication)

2nd International Conference on X-Chromosome InactivationSeptember 17th-22nd, 2006Paris, France(Poster presentation)

Cold Spring Harbor Laboratory Molecular Embryology of the Mouse CourseJune 7-27th, 2006Cold Spring Harbor, USA

EMBO Workshop on Functional Organization of the Cell NucleusMay 5-8th, 2006Prague, Czech Republic(Poster presentation and short talk)

EuroSciCon meeting on "Visualising transcription, gene positioning and reorganisation in thenucleus"February 24th, 2006UCL, London, England

Jacques Monod Meeting on "Epigenetics in Development and Disease : perspectives from multiple organisms"January 4-8th, 2006CNRS, Aussois, France(Poster presentation)

Meeting on "Genes regulation : from chromatin remodelling to transcription"November 24-25th, 2005Institut de Biologie Intégrative, Paris, France(Poster presentation)

Scientific Meeting of the Departement of Developmental Biology of the Pasteur InstituteJanuary 18-19th, 2005Institut Pasteur, Paris, France(Oral communication)

Course on Cellular and Molecular Dynamics ImagingDecember 1-4th, 2003Institut Jacques Monod, Paris, France

ACI meeting on "Integrative Biology"January 2003Collège de France, Paris, France(Poster presentation)

Teaching and mentoring

Mentoring undergraduate and rotation students

2008-present

Students: Kristine Wong, Amy Campbell, Tuongvi Pham, Robert Plasshaert

Marisa Bartolomei's Laboratory, University of Pennsylvania School of Medicine

Mentoring middle school students with iPraxis

2008-present

Discussion leader for the Penn Reading Project

2008, 2009

Publications

CTCF binding site classes exhibit distinct evolutionary, genomic, epigenomic and transcriptomic features.

Essien K#, Vigneau S#, Apreleva S#, Singh LN, Bartolomei MS, Hannenhalli S. (# these authors contributed equally)

Genome Biol. 2009 Nov 18;10(11):R131. pubmed

A role for non-coding Tsix transcription in partitioning chromatin domains within the mouse X-inactivation centre.

Navarro P, Chantalat S, Foglio M, Chureau C, Vigneau S, Clerc P, Avner P, Rougeulle C.

Epigenetics Chromatin. 2009 Jul 20;2(1):8. pubmed

Genomic imprinting mechanisms in mammals.

Ideraabdullah FY, Vigneau S, Bartolomei MS.

Mutat Res. 2008 Dec 1;647(1-2):77-85. Review. pubmed

H19 in the pouch

Bartolomei MS, Vigneau S, O'Neill MJ.

Nat Genet. 2008 Aug;40(8):932-3. Review. pubmed

Without Tsix, male X chromosome is inactivated.

Vigneau S, Clerc P.Med Sci (Paris). 2006 Nov;22(11):926-8. Review. pubmed

An essential role for the DXPas34 tandem repeat and Tsix transcription in the counting process of X-chromosome inactivation.

Vigneau S, Augui S, Navarro P, Avner P, Clerc P.Proc Natl Acad Sci U S A. 2006 May 9;103(19):7390-5. pubmed

Multiple origins of Cajal-Retzius cells at the borders of the developing pallium.

Bielle F, Griveau A, Narboux-Nême N, Vigneau S, Sigrist M, Arber S, Wassef M, Pierani A.Nat Neurosci. 2005 Aug;8(8):1002-12. pubmed

Grants and fellowships

Hobbies

Dancing (lindy-hop, tango, salsa...)

Playing saxophone

Climbing

Languages

French (native speaker), English (fluent), Spanish (fluent), Portuguese (notions), Romanian (notions).

Skills

Skills

Computer Skills

Operating Systems: Linux, MS Windows, Mac OS X. Programming: Unix Shell Script, Perl, Java, Scheme, R, SQL, HTML, CSS, Javascript, LaTeX.   Relational database: SQL, MS Access, FileMaker Pro, Zoho Creator.   Bioinformatics: Sequence editors, online bioinformatics databases and tools.   Imaging: Volocity, ImageJ. Office:  Open Office, MS Office, LaTeX.   Project management: dotProject, OpenAtrium.   CMS: Wordpress, Drupal.   Graphics: Gimp, Inkscape, Adobe Photoshop and Illustrator.

Biological Skills

Cellular Genetics: molecular cloning and recombineering; mouse ES cell culture and differentiation; generation and screening of recombinant ES cells. Transcriptional Analysis and Imaging: real-time quantitative RT-PCR; chromatin immunoprecipitation; allelic assays; DNA, RNA and IF FISH; in situ hybridization and immunocytochemistry on whole-mount embryos and cryostat sections; transmission, fluorescence and confocal microscopy. Embryology: Manipulation of mouse oocytes and embryos (pre- and post-implantation); ex vivo culture of brain explants; cell lineage and migration tracing with DiI labelling; initiated to a broad range of embryology techniques in the Cold Spring Harbor Laboratory Molecular Embryology of the Mouse course.  

Certifications

Certifications
Sep 2004 - Jul 2007

Certificate of competency in Bioinformatics

Conservatoire National des Arts et Métiers