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Sandra Goetze

Lead Senior Scientist biomedical Proteomics

Summary


Experience

Researcher

CTI Proteomedix
Apr 2015Present
Members:, Bernd Wollscheid, Ralph Schiess, Daniel Gygax Worldwide approximately 900' 000 men are diagnosed with PCa annually and 85% of these cancers are detected in men with early-stage disease. Most of these cases are non-aggressive and the cancer is localized and has not spread outside the prostate gland. Nevertheless, the majority of patients undergo immediate treatment such as prostatectomy and radiotherapy because of the lack of prognostic tools. Overtreatment of PCa, the most prevalent cancer in men, is driving health care costs and bears risks for patients. The diagnostic tools developed by ProteoMediX will reduce overtreatment by providing urgently needed diagnostic tests that supports physicians in their therapy decision making(immediate treatment or active surveillance). The goal of the interdisciplinary group including ProteoMediX and the research groups Prof.

Analyst

Authors
Dec 2014Present
J Proteome Res.:, Jérôme Garin, Dorothée LEBERT, louwagie mathilde, Guillaume Picard, Caroline Duquesne, ferro myriam, Oliver Rinner, Ruedi Aebersold, mouz nicolas, Virginie B. In bottom-up mass spectrometry-based proteomics analyses, variability at any step of the process, particularly during sample proteolysis, directly affects the sensitivity, accuracy, and precision of peptide detection and quantification. Currently, no generic internal standards are available to control the quality of sample processing steps. This makes it difficult to assess the comparability of MS proteomic data obtained under different experimental conditions. Here, we describe the design, synthesis, and validation of a universal Page2 protein standard, called DIGESTIF, that can be added to any biological sample. The DIGESTIF standard consists of a soluble recombinant protein scaffold to which a set of 11 artificial peptides(iRT peptides) with good ionization properties has been incorporated. In the protein scaffold, the amino acids flanking iRT peptide cleavage sites were selected either to favor or hinder protease cleavage. After sample processing, the retention time and relative intensity pattern of the released iRT peptides can be used to assess the quality of sample workup, the extent of digestion, and the performance of the LC-MS system. Thus, DIGESTIF can be used to standardize a broad spectrum of applications, ranging from simple replicate measurements to large- scale biomarker screening in biomedical applications. Protection of armadillo/#-Catenin by armless, a novel positive regulator of wingless signaling.

Authors

Authors
Nov 2014Present
PLoS biology: Gerlinde Reim, Martina Hruzova,, Konrad Basler signaling pathway is essential for metazoan development, where it is central to tissue growth and cellular differentiation. Deregulated Wg pathway activation underlies severe developmental abnormalities, as well as carcinogenesis. Armadillo/#-Catenin plays a key role in the Wg transduction cascade; its cytoplasmic and nuclear levels directly determine the output activity of Wg signaling and are thus tightly controlled. In all current models, once Arm is targeted for degradation by

Kathryn Lilley, Boris Adryan University of Cambridge

Kathryn Lilley, Boris Adryan University of Cambridge
Apr 2015Present
Great Britain) Alexey Nesvizhskii, Chih-Chiang Tsou(University of Michigan; USA) Zurich, (S. Goetze) List of Publications (a) Referred Journals/Book contributions

Researcher

Authors
Nov 2009Present
PLoS biology Page4:, Ermir Qeli, Christian Mosimann, An Staes, Bertran Gerrits, Bernd Roschitzki, Sonali Mohanty, Eva M Niederer, Endre Laczko, Evy Timmerman, et al Protein modifications play a major role for most biological processes in living organisms. Amino-terminal acetylation of proteins is a common modification found throughout the tree of life: the N-terminus of a nascent polypeptide chain becomes co-translationally acetylated, often after the removal of the initiating methionine residue. While the enzymes and protein complexes involved in these processes have been extensively studied, only little is known about the biological function of such N-terminal modification events. To identify common principles of N-terminal acetylation, we analyzed the amino-terminal peptides from proteins extracted from Drosophila Kc167 cells. We detected more than 1, 200 mature protein N-termini and could show that N-terminal acetylation occurs in insects with a similar frequency as in humans. As the sole true determinant for N-terminal acetylation we could extract the(X) PX rule that indicates the prevention of acetylation under all circumstances. We could show that this rule can be used to genetically engineer a protein to study the biological relevance of the presence or absence of an acetyl group, thereby generating a generic assay to probe the functional importance of N-terminal acetylation. We applied the assay by expressing mutated proteins as transgenes in cell lines and in flies. Here, we present a straightforward strategy to systematicallystudythe functional relevance of N-terminal acetylations in cells and whole organisms. Since the(X) PX rule seems to be of general validity in lower as well as higher eukaryotes, we propose that it can be used to study the function of N-terminal acetylation in all species. Organization of the amplified type I interferon gene cluster and associated chromosome regions in the interphase nucleus of human osteosarcoma cells. Chromosome research: an international journal on the molecular, supramolecular and evolutionary aspects of

Authors

Authors
Mar 2009Present
Proceedings of the National Academy of Sciences of the United States of America: Julio Mateos-Langerak, Manfred Bohn, Wim de Leeuw, Osdilly Giromus, Erik M M Manders, Pernette J Verschure, Mireille H G Indemans, Hinco J Gierman, Dieter W Heermann, Roel van Driel, Genome function in higher eukaryotes involves major changes in the spatial organization of the chromatin fiber. Nevertheless, our understanding of chromatin folding is remarkably limited. Polymer models have been used to describe chromatin folding. However, none of the proposed models gives a satisfactory explanation of experimental data. In particularly, they ignore that each chromosome occupies a confined

Kathryn Lilley, Boris Adryan University of Cambridge

Kathryn Lilley, Boris Adryan University of Cambridge
Apr 2015Present
Great Britain) Alexey Nesvizhskii, Chih-Chiang Tsou(University of Michigan; USA) Zurich, (S. Goetze) List of Publications (a) Referred Journals/Book contributions

Authors

Authors
Mar 2004Present
Journal of cell science: Henry H Q Heng,, Christine J Ye, Guo W Bremer, Susan M Wykes, Juergen Bode, Stephen A Krawetz

Authors

Authors
Apr 2006Present
Journal of molecular biology: Prashanth A K, Craig Benham Scaffold or matrix-attachment regions(S/MARs) are thought to be involved in the organization of eukaryotic chromosomes and in the regulation of several DNA functions. Theircharacteristics are conserved between plants and humans, and a variety of biological activities have been associated with them. The identification of S/MARs within genomic sequences has proved to be unexpectedly difficult, as they do not appear to have consensus sequences or sequence motifs associated with them. We have shown that S/MARs do share a characteristic structural property, they have a markedly high predicted propensity to undergo strand separation when placed under negative superhelical tension. This result agrees with experimental Page8 observations, that S/MARs contain base-unpairing regions(BURs). Here, we perform a quantitative evaluation of the association between the ease of stress-induced DNA duplex destabilization(SIDD) and S/MAR binding activity. We first use synthetic oligomers to investigate how the arrangement of localized unpairing elements within a base-unpairing region affects S/MAR binding. The organizational properties found in this way are applied to the investigation of correlations between specific measures of stress- induced duplex destabilization and the binding properties of naturally occurring S/MARs. For this purpose, we analyze S/MAR and non-S/MAR elements that have been derived from the human genome or from

Co-localization of PARP-1

Co-localization of PARP-1
Oct 2005Present
and lamin B in the nuclear architecture: a halo-fluorescence-and confocal- microscopy study. Journal of cellular biochemistry

Lecturer

University of Zurich Authors
20122014
Lecturer at (2 years)

Senior Research Assistant

Zurich
Jul 2008Dec 2013
Switzerland Marie-Heim Vögtlin fellow, Senior Research Assistant Fields of research: The Wnt Signaling Cascade Targeted/Quantitative Proteomics Analysis of Functional Chromatin Complexes

Senior Scientist

University of Zurich Authors
Jul 2008Dec 2013
Senior Scientist(Marie Heim-Vögtlin Fellow) at (5 years 6 months)

Senior Research Assistant

Zurich
Jul 2008Dec 2013
Switzerland Marie-Heim Vögtlin fellow, Senior Research Assistant Fields of research: The Wnt Signaling Cascade Targeted/Quantitative Proteomics Analysis of Functional Chromatin Complexes

University of Amsterdam Authors

University of Amsterdam Authors
Apr 2007Jun 2008
1 year 3 months) Postdoc at

Centre for Infection Research Authors

Centre for Infection Research Authors
Jan 2004Dec 2006
3 years) Postdoc at Helmholtz

MH, Gierman HJ, Heermann DW

MH, Gierman HJ, Heermann DW
20032003
Bode J, Goetze S, Heng H, Krawetz, Benham C: From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics. Chromosome Res 2003, 11: 435-445. Goetze S, Huesemann Y, Baer A, Bode J: Functional characterization of transgene integration patterns by halo fluorescence in situ hybridization: electroporation versus retroviral infection. Biochemistry, 42: 7035-7043. Goetze S, Gluch A, Benham C, Bode J: Computational and in vitro analysis of destabilized DNA regions in the interferon gene cluster: potential of predicting functional gene domains. Biochemistry, 42: 154-166. Bode J, Goetze S, Ernst E, Husemann Y, Baer A, Seibler J, Mielke C: Architecture and utilization of highly expressed genomic sites. New Comprehensive Biochemistry. edn Volume 38. Edited by: Elsevier; 2003: 551-572. Goetze S, Baer A, Winkelmann S, Nehlsen K, Seibler J, Maass K, Bode J: Performance of genomic bordering elements at predefined genomic loci. Mol Cell Biol 2005, 25: 2260-2272. Vidakovic M, Koester M, Goetze S, Winkelmann S, Klar M, Poznanovic G, Bode J: Co-localization of PARP-1 and lamin B in the nuclear architecture: a halo-fluorescence-and confocal-microscopy study. J Cell Biochem 2005, 96: 555-568. Bode J, Winkelmann S, Gotze S, Spiker K, Tsutsui K, Bi C, A KP, Benham C: Correlations Between Scaffold/Matrix Attachment Region(S/MAR) Binding Activity and DNA Duplex Destabilization Energy. J Mol Biol 2006, 358: 597-613 Gladilin E, Goetze S, Mateos-Langerak J, van: Topological analysis of 3D cell nuclei using finite element template-based spherical mapping. Proceedings of SPIE 2006, 6144: 1557-1566 Winkelmann S, Klar M, S, Gluch A, Bode J: The positive aspects of stress: Strain Initiates Domain Decondensation(SIDD). Briefings in Functional Genomics and Proteomics 2006, 5: 24-31 Gladilin E, Goetze S, van Driel R, Eils R: Stochastical analysis of finite point sampling of the 3D chromatin fiber in the interphase nucleus. BIRD 2007, 104-118 Goetze S, Mateos-Langerak J, Gierman HJ, de Leeuw W, Giromus O, Indemans MHG

MH, Gierman HJ, Heermann DW

MH, Gierman HJ, Heermann DW
20032003
Bode J, Goetze S, Heng H, Krawetz, Benham C: From DNA structure to gene expression: mediators of nuclear compartmentalization and dynamics. Chromosome Res 2003, 11: 435-445. Goetze S, Huesemann Y, Baer A, Bode J: Functional characterization of transgene integration patterns by halo fluorescence in situ hybridization: electroporation versus retroviral infection. Biochemistry, 42: 7035-7043. Goetze S, Gluch A, Benham C, Bode J: Computational and in vitro analysis of destabilized DNA regions in the interferon gene cluster: potential of predicting functional gene domains. Biochemistry, 42: 154-166. Bode J, Goetze S, Ernst E, Husemann Y, Baer A, Seibler J, Mielke C: Architecture and utilization of highly expressed genomic sites. New Comprehensive Biochemistry. edn Volume 38. Edited by: Elsevier; 2003: 551-572. Goetze S, Baer A, Winkelmann S, Nehlsen K, Seibler J, Maass K, Bode J: Performance of genomic bordering elements at predefined genomic loci. Mol Cell Biol 2005, 25: 2260-2272. Vidakovic M, Koester M, Goetze S, Winkelmann S, Klar M, Poznanovic G, Bode J: Co-localization of PARP-1 and lamin B in the nuclear architecture: a halo-fluorescence-and confocal-microscopy study. J Cell Biochem 2005, 96: 555-568. Bode J, Winkelmann S, Gotze S, Spiker K, Tsutsui K, Bi C, A KP, Benham C: Correlations Between Scaffold/Matrix Attachment Region(S/MAR) Binding Activity and DNA Duplex Destabilization Energy. J Mol Biol 2006, 358: 597-613 Gladilin E, Goetze S, Mateos-Langerak J, van: Topological analysis of 3D cell nuclei using finite element template-based spherical mapping. Proceedings of SPIE 2006, 6144: 1557-1566 Winkelmann S, Klar M, S, Gluch A, Bode J: The positive aspects of stress: Strain Initiates Domain Decondensation(SIDD). Briefings in Functional Genomics and Proteomics 2006, 5: 24-31 Gladilin E, Goetze S, van Driel R, Eils R: Stochastical analysis of finite point sampling of the 3D chromatin fiber in the interphase nucleus. BIRD 2007, 104-118 Goetze S, Mateos-Langerak J, Gierman HJ, de Leeuw W, Giromus O, Indemans MHG

Education

PHD Doctorate

20002003

PHD Doctorate

2003

PHD Doctorate

2003

Diploma

Technical University Braunschweig
1999

Diploma

Technische Universität Braunschweig
19941999

Diploma

Technical University Braunschweig
1999

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