Rupakula Aamani

Lausanne, Switzerland
[email protected]

Work experience

Feb 2011Present

PhD student

Ecole Polytechnique Fédérale de Lausanne (EPFL)

environmental microbiology, biotechnology, biochemistry of anaerobes, organohalide respiration, dehalobacter restrictus, corrinoid, Reductive dehalogenase

The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics

Rupakula - Kruse et. al., Philos Trans R Soc Lond B Biol Sci 368:20120325. 2013

Dec 2009May 2010

Master's thesis Research at Laboratory for Environmental Biotechnology

EPFL

“Purification & characterization of PceC component of Reductive Dehalogenase of Desulfitobacterium hafniense strain TCE1 & Dehalobacter spp. “

Education

20082010

Masters of science

Vellore Institute of Technology, India

20052008

Bachelor of Science (B.Sc.)

NVPAS, S P university, India

20032005

CBSE high school

Sanskriti Civil Services School, Chanakya Puri, New Delhi, India

Jan 2002Dec 2003

High school

Mpepu college, Molepolole, Botswana, Africa

GCSE Cambridge "O" level certificate

Skills

Biotechnology

Experience, Skills

Environmental Microbiology and Biotechnology
Bioremediation
Biochemistry of Anaerobes
Organo Halide Respiration
Dehalobacter restrictus
Corrinoid Biosynthesis and Salvage
Reductive Dehalogenases
Transcriptional regulation
Regulator proteins
Molecular biology

Gel electrophoresis (SDS, Native, gel shift mobility assays)
Western blotting
Genomics: Functional annotation of genes and metabolic pathways
Comparative genomics, Transcriptomics, Proteomics
Anaerobic cell culture
Regulation of Gene Expression
Heterologous Protein Expression
Differential gene expression
Identifying protein-ligand interactions
Isothermal Titration Calorimetry
Cloning
Reverse transcription
Quantitative PCR
Sequencing
DNA RNA extraction and Optimization of method for cells with low biomass