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Work experience

Feb 2011Present

PhD student

Ecole Polytechnique Fédérale de Lausanne (EPFL)

environmental microbiology, biotechnology, biochemistry of anaerobes, organohalide respiration, dehalobacter restrictus, corrinoid, Reductive dehalogenase

The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics

Rupakula - Kruse et. al., Philos Trans R Soc Lond B Biol Sci 368:20120325. 2013

Dec 2009May 2010

Master's thesis Research at Laboratory for Environmental Biotechnology

EPFL

“Purification & characterization of PceC component of Reductive Dehalogenase of Desulfitobacterium hafniense strain TCE1 & Dehalobacter spp. “ 

Education

20082010

Masters of science

Vellore Institute of Technology, India
20052008

Bachelor of Science (B.Sc.)

NVPAS, S P university, India
20032005

CBSE high school

Sanskriti Civil Services School, Chanakya Puri, New Delhi, India
Jan 2002Dec 2003

High school

Mpepu college, Molepolole, Botswana, Africa

GCSE Cambridge "O" level certificate

Skills

Biotechnology

Experience, Skills

  • Environmental Microbiology and Biotechnology
  • Bioremediation
  • Biochemistry of Anaerobes
  • Organo Halide Respiration
  • Dehalobacter restrictus
  • Corrinoid Biosynthesis and Salvage
  • Reductive Dehalogenases
  • Transcriptional regulation
  • Regulator proteins
  • Molecular biology
  • Gel electrophoresis (SDS, Native, gel shift mobility assays)
  • Western blotting
  • Genomics: Functional annotation of genes and metabolic pathways
  • Comparative genomics, Transcriptomics, Proteomics
  • Anaerobic cell culture
  • Regulation of Gene Expression
  • Heterologous Protein Expression
  • Differential gene expression
  • Identifying protein-ligand interactions
  • Isothermal Titration Calorimetry
  • Cloning
  • Reverse transcription
  • Quantitative PCR
  • Sequencing
  • DNA RNA extraction and Optimization of method for cells with low biomass