Rupakula Aamani

Rupakula Aamani

Work History

Work History
Feb 2011 - Present

PhD student

Ecole Polytechnique Fédérale de Lausanne (EPFL)

environmental microbiology, biotechnology, biochemistry of anaerobes, organohalide respiration, dehalobacter restrictus, corrinoid, Reductive dehalogenase

The restricted metabolism of the obligate organohalide respiring bacterium Dehalobacter restrictus: lessons from tiered functional genomics

Rupakula - Kruse et. al., Philos Trans R Soc Lond B Biol Sci 368:20120325. 2013

Dec 2009 - May 2010

Master's thesis Research at Laboratory for Environmental Biotechnology

EPFL

“Purification & characterization of PceC component of Reductive Dehalogenase of Desulfitobacterium hafniense strain TCE1 & Dehalobacter spp. “ 

Education

Education
2008 - 2010

Masters of science

Vellore Institute of Technology, India
2005 - 2008

Bachelor of Science (B.Sc.)

NVPAS, S P university, India
2003 - 2005

CBSE high school

Sanskriti Civil Services School, Chanakya Puri, New Delhi, India
Jan 2002 - Dec 2003

High school

Mpepu college, Molepolole, Botswana, Africa

GCSE Cambridge "O" level certificate

Skills

Skills

Biotechnology

Experience, Skills

Areas of experience (general & specific):
  • Environmental Microbiology and Biotechnology
  • Bioremediation
  • Biochemistry of Anaerobes
  • Organo Halide Respiration
  • Dehalobacter restrictus
  • Corrinoid Biosynthesis and Salvage
  • Reductive Dehalogenases
  • Transcriptional regulation
  • Regulator proteins
  • Molecular biology
Skills:
  • Gel electrophoresis (SDS, Native, gel shift mobility assays)
  • Western blotting
  • Genomics: Functional annotation of genes and metabolic pathways
  • Comparative genomics, Transcriptomics, Proteomics
  • Anaerobic cell culture
  • Regulation of Gene Expression
  • Heterologous Protein Expression
  • Differential gene expression
  • Identifying protein-ligand interactions
  • Isothermal Titration Calorimetry
  • Cloning
  • Reverse transcription
  • Quantitative PCR
  • Sequencing
  • DNA RNA extraction and Optimization of method for cells with low biomass
Teaching Assistant : Organizational skills:
  • Research project management
  • Methodology development
  • Writing scientific papers and reports
Social skills:
  • Team-building and leadership experience
  • Ability to grow and work in an interdisciplinary environment
  • Problem-solving skills
  • Strong listening and expression skills
  • Open-minded and adaptability
  • Ability to initiate and foster collaborations
  • Maintain inter-personal skills
  • Enjoy simplifying Science
  • Possess strong communication and Oral-presentation skills.