Summary

Molecular Biologist with more than 8 years experience in all types of protein isolation, characterization and analysis by different techniques like PAGE, 2-DE, MALDI-TOF, Orbitrap, Western Blotting, ELISA, etc. Posses excellent knowledge on DNA and RNA isolation and downstream processes like PCR and RT-PCR and  microscopic technique.  Has also acquired excellent knowledge in animal handling and tissue culture experiments.

Work History

Work History
Jul 2014 - 2016

Research Associate of Dept. Of Biotechnology,Govt. of India 

Dept. of Biotechnology, Govt. of India

Working as Research Associate (DBT funded) in the Department of Organic Chemistry, in the Indian Association for the Cultivation of Science, Jadavpur, Kolkata, India-700032.

The focus of this work is the study of non-peptide based morpholinos (novel design for the purpose of cell penetration) and small molecule induced inhibition of Hedgehog signalling pathways in Cancer cells and zebrafish embryos.

Jun 2013 - Jun 2014

Post Doctoral Research Associate

Centre for Cellular and Molecular Biology, Hyderabad, India-500007

The first PDF was at CCMB where, I had the opportunity to work on the proteome mapping of Crocodylus palustris (Indian mugger), embryonic brain and reproductive tissues. The primary aim of that project was to map the proteins that are vital  for the determination of sex organ in these organism where the main determining factor for sex is temperature.

Mar 2011 - May 2013

Research Fellowship in Sciences for meritorious students 

University Grant Commission, Govt. of India

The effect of PKA inhibition during maturational process in climbing Perch (Anabas testudineus) oocytes

The study involves the mapping of the cellular pathways that are involved in the process of development  of a primary oocyte to a developed ovum.  

2009 - 2010

Senior Research Fellow

Department of Science and Technology, Govt of India

Continuation of the PhD project work as  DST funded Senior Research Fellow

2007 - 2009

Junior Research Fellow

Department of Science and Technology

Junior Research Fellow in funded project“ Regulation of maturation (Anabas testudineus) oocytes.

Education

Education
2007 - 2013

PHD Doctorate

Visva-Bharati, Santiniketan, West Bengal, India-731235
2006 - 2007

MPhil Doctorate

University of Calcutta, West Bengal, India

MPhil in Fisheries Technology with First division

2005

M.Sc in Zoology

Visva-Bharati, Santiniketan, West Bengal, India-731235

Zoology (Hons) with specialization in Fish Biology with First Division

Skills

Skills

Molecular Biology Skills

Proficiency in molecular biology techniques like

  •  DNA/RNA/Protein isolation
  • PCR, RT-PCR, primer designing
  • Cloning and gene expression
  • Cell transfection 
  • One Dimensional and Two Dimensional PolyAcrylamide Gel Electrophoresis and downstream processing such as in-gel and in-solution sample preparation for MALDI-TOF and Orbitrap LC/MS.  

Animal Handling and Tissue culture

Trained in handling primary cell culture and cell line maintenance. Handling animal models like mouse and fishes. Also,operated microinjection setup for the delivery of Morpholino compounds into zebrafish embryos at its different developmental stages.

Microscopic Techniques

 in handling Light/Fluorescence/Phase Contrast Microscope and Confocal microscopy.

Enzymatic and In vitro assay

Well conversant in designing and carrying out of Radioactive Kinase Assays, ELISA,  Immuno-histochemistry and Immuno-Cytochemistry.

Organizational and communicative skills

Has good written and communication skills to lead a group.

Conversant with major language and software and operating systems, Microscope imaging software and advanced Proteomic software like SEQUEST, Proteome Discoverer, SEIVE and Mascot.  Efficient in handling large scale MS data processing

Publication

  • Khan, P.P. , Maitra, S., 2013. Participation of cAMP-dependent protein kinase and MAP kinase pathways during Anabas testudineus oocyte maturation. General and Comparative Endocrinology, 181 ,88–97.
  • DasD., Khan, P.P. , Maitra,S., 2013. Participation of PI3-kinase/Akt signalling in insulin stimulation of p34cdc2 activation in zebrafish oocyte: Phosphodiesterase 3 as a potential downstream target. Molecular and Cellular Endocrinology ,374 ,46–55
  • Khan, P.P., 2012. Isozyme polymorphism analysis due to Lead and Zinc toxicity: A report on Mrigal (Cirrhinus mrigala, Hamilton). [ISBN no. 978-3-659-17487-2]. Lambert Academic Publishing, Heinrich-Böcking-Str.6-8, 66121, Saarbrücken, Germany.
  • Das, D., Khan, P.P., Maitra,S., 2016. Endocrine and paracrine regulation of meiotic cell cycle progression in teleost oocytes: cAMP at the centre of complex intra-oocyte signalling events. General and Comparative Endocrinology, DOI: 10.1016/j.ygcen.2016.01.005.


PERSONAL DOSSIER

Date of Birth:  14th of July,1980

Sex:   Female

Religion :   Hindu (General Caste)

Marital Status:  Unmarried

Nationality :  Indian

Language known:  English (US) (UK), Hindi, Bengali

Address   (O):Indian Association for Cultivation of Sciences, 2A&2B Raja S. C. Mallick Road, Jadavpur,                                   Kolkata, West Bengal

                    (R):17, Kalibari Lane (Fl 1B), Jadavpur, Kolkata, West Bengal.   India-7000032.

References

References

Dr. Sudipta Maitra (PhD Supervisor)

Associate Professor

Department of Zoology

Visva-Bharati, Santiniketan,

West Bengal,

India-731235.

Email: smaitra3@gmail.com

Telephone: +91-9874405555

Prof. Samir Bhattacharya (PhD Supervisor)

Senior Professor

Department of Zoology

Visva-Bharati, Santiniketan,

West Bengal,

India-731235.

Email: bhattacharyasa@gmail.com

Telephone::+91-03463-261176

Dr. Surojit Sinha

Scientist

Indian Association for Cultivation of Sciences

2A&2B Raja S. C. Mallick Road, Jadavpur,

Kolkata,

 West Bengal,

India-700032.

Email: ocss5@iacs.res.in