Pavankumar Varanasi

Pavankumar Varanasi

Work History

Work History
May 2010 - Aug 2010

Research Project

Nottingham Trent University



To determine the activity of Transglutaminase in germinating wheat aleuronic layers, extracted samples were prepared from the germinating wheat aleuronic layers and Transglutaminse (Tgase) specific activity and presence of Tgases was determined for the extracted samples. By different procedures we identified Transglutaminase from the extracted samples.This was achieved by the following procedures 1. Samples were extracted in liquid nitrogen, 2. Extracted samples were assayed by Biotin Caderevine assay and protein assay to check the specific activity Tgase, 3. Blotting the samples on nitrocellulose (NC) membrane and identifying the cross reactivity of the extracted Tgases by using specific HRP conjugated immunoglobulin G (IgG) anti-mouse antibody. 4. Antibody specific to the Tgase was identified by testing the standard and concentrated extracted samples by blotting on NC membrane and cross reactivating with different antibodies. 5. Checking out the activity of PA’s by histochemical localisation of the enzyme in the germinated aleuronic layers of the wheat seeds. The activity of the Tgase was found to be increased in the Ca2+ ions. Increase in the activity of the Tgases in Gibberellic acid was found and it was more than the Ca2+ ions. The molecular weight of the Tgase was predicted by SDS-PAGE. Antibody specific to the Tgases was identified by using dot blot and the presence of Tgases in the aleuronic layers was demonstrated by histochemical staining.

Key words: Wheat, aleuronic layers, Transglutaminase (Tgase), Gibberellic acid (GA)

Apr 2009 - Oct 2009

Research Assistant

Acharya Ranga Agricultural University
  • Worked in Plant Cell Culture, making up cultures, maintining the cultures and regular observation of the cultures.
  • Analysing the prepares samples and product yield using various techniques such as HPLC, GC and UV spectrophotometer.
  • Carrying various Immunoassays, DNA extraction, Amplification, Transformation and Cloning.
  • Trained in Invitro Crystallisation




MS Office Windows Mini Tab C


Molecular Cell Biology:   DNA Extraction and Purification   PCR   Cloning   Transformation   Screening   Biochemical and Analytical Techniques:          HPLC          GC   Native Protein extraction and Purification   SDS PAGE Electrophoresis   Western Blot   Protein Identification   Dot Blot   Chemiluminiscance       UV and Nano drop Spectrophotometer Bioinformatics Skills:   Experience in retrieving and analyzing DNA, protein sequences and structures from databases.   Phylogenetics.   Familiar with the database search   Hands on experience in PYMOL, AUTODOCK  and RASMOL.   Methodologies. Docking and Molecular modelling   Immunology Techniques:   ELISA   Radio Immunoassay   Histochemical Staining   Protein Staining   Animal Biotechnology:   Primary Cell Culture Preparation using brain and liver cells.   Plant Biotechnology:       Callus Production


Oct 2009 - Nov 2010


Nottingham Trent University

Core Modules:

  • Biology of Disease (Cancer and Heart)
  • Cell Culture and Antibody Technology
  • Enzyme Technology and Antibody Catalysis
  • Molecular Biologya ant DNA Technology
  • Research Methods and IT
Jul 2006 - Apr 2009


University of Madras

Core Subjects:

  • Cell Biology
  • Biochemistry
  • Molecular Cell Biology
  • Plant Biotechnology
  • Animal Biotechnology
  • Bioprocess and Engineering
  • Genetics
  • Genetic Engineering


I am seeking an opportunity to become a part of the research team, engaged in study of Molecular mechanisms, Chemistry and Bioinformatics. I am keen to broaden my knowledge and build on my existing skills and experience to date.

Other Activities

  • Cancer and AIDS Awareness events
  • School Events organiser to create awareness in AIDS.
  • Worked as a cancer fund raiser at Nottingham.
  • Good team worker
  • Event Organisation
  • Captain for a Cricket Team (Junior College Team)

Graduate Coursework & Presentation

Bioleaching best way for the extaction of minerals:

My first presentation given in the National Seminar during my BSc at Jaya College of Arts and Science explaining the best and easy ways of the extraction of metals and minerals from the ores. Which reduces the labour and eco friendly as well.

Presentations as a Part of Academics MSc:

Presentation on the role of CD36 and foam Cell formation during the cardiovascular disease as a part of my academics at Nottingham Trent university 2009.

Phage display technology: A rapid and efficient strategy to generate antigen-specific human monoclonal antibody by invitro immunization and Phage display method.

Courseworks a Part of Academics MSc:

  • Role of Microbialenzymes in the non-diary food industry.
  • Ethics and Issues in Animal Experimentation.


  • I am facinated how all the molecular development takes form a single cell to organ. Any changes in the during the develpment leads to abnormalities and results in severe damage and ends up in different Cancers. I would like to study in detail the molecular mechanisms that leads to the cancer and develop an effective diagnostic procedure.
  • In the same time I wounder how plants can adapt to different fluctations in environment, pests and viruses. During these conditions at a time in the plant shows cross talk during adaptation. My interest  is in identification of the genes reponsible for cross talk and to develop plants effective to all the conditions at a time.



Shiva Sivasubramaniam

MSc Pharmocology Programme leader, Seniour Lecturer

Philip Bonner

MRes Programme Leader,

MSc project supervisor