Ombretta Repetto

Ombretta Repetto

Work History

Work History
Jul 2011 - Present

Researcher

CRO-National Cancer Institute, Aviano (PN), Italy

The Facility of Bio-Proteomics provides an advanced scientific and technical support in differential protein profiles and protein identification for both basic and applied research. It is conceived as a Laboratory in the S.O.C. Clinical and Experimental Pharmacology with the following mission: 1. to create a multidisciplinary and collaborative research group with the capabilities to identify proteins by ‘Matrix Assisted Laser Desorption/Ionization Time-of-Flight, MALDI-TOF’ mass spectrometry; 2. to analyze differential protein expression profiles and to identify proteins of interest from tissue, biological fluid and/or cell lines by peptide mass fingerprinting (PMF); 3. to search for potential protein biomarkers and/or targets for therapeutics in cancer or other major diseases.

Repetto O, De Paoli P, De Re V, Vincenzo Canzonieri V, and Cannizzaro R. 2014. Levels of Soluble E-Cadherin in Breast, Gastric, and Colorectal Cancers. Review Article. BioMed Research International. Volume 2014. Article ID 408047, 7 pages http://dx.doi.org/10.1155/2014/408047

Repetto O, Zanussi S, Casarotto M, Canzonieri V, De Paoli P, Cannizzaro R, De Re V. 2013. Differential proteomics of Helicobacter pylori associated with autoimmune atrophic gastritis. Mol Med. doi: 10.2119/molmed.2013.00076.

De Re V, Caggiari L, Garziera M, De Zorzi M and Repetto O. 2012. Molecular signature in HCV-positive lymphoma. Clinical and Developmental Immunology. 2012;2012:623465. doi: 10.1155/2012/623465. Epub 2012 Aug 16. Review. PMID:22952554.

Jan 2009 - Nov 2010

Researcher. Proteomics and Mass Spectrometry R&D

ISB Srl, Milan, Italy

I worked for the service of proteomics, from sample preparation for mass spectrometry with experimental settings, to MS data elaboration, protein identifications and result interpretation at the biological level, and report redaction for Customers. I wrote the ufficial versions of laboratory internal Materials and Methods, and actively contributed to the redaction of projects, scientific papers and Congress oral presentations/posters. Moreover, since January of this year, because of my experience in Proteomics and my own communication and relational skills, I was in charge for a commercial activity aimed to search for new customers in Italy for plant proteomics, by writing Services brochures, planning Meeting and giving Oral Presentations, besides analytical assistance and technical advices.

Cristoni S, Molin L, Lai A, Rossi Bernardi L, Pucciarelli S, Agostini M, Bedin C, Nitti D, Seraglia R, Repetto O, Dibari VF, Orlandi R, Martínez-Lozano Sinues P, Traldi P. 2009. Maldi-MS-NIST library approach for colorectal cancer diagnosis. Rapid Communications in Mass Spectrometry 23:2839-2845.

Jul 2006 - Aug 2008

Research fellowship. Grapevine physiological and molecular characterization responses towards pathogens

CRA-VIT, Centre for Research in Viticulture

I was in charge of a research activity in the field of grapevine responses towards pathogens (downy mildew and leafroll associated-virus 3) and its characterization by molecular biology (RNA and DNA extraction, PCR, RFLP, ELISA), physiological parameters and biochemistry (HPLC - resveratrol and other stilbenes), under the supervision of Dr. Michele Borgo and Dr. Elisa Angelini. In particular, I collaborated with other collegues to characterize possible virus-3 induced modifications  in grapevine behaviour against the late infections by Plasmopara viticola in field, candidate responsive molecules being searched in several metabolic pathways.

Repetto O, Bertazzon N, Miotti L, De Rossi M, Miotti L, Flamini R, Angelini E, Borgo M. 2012. Low susceptibility of grapevine infected by GLRaV-3 to late Plasmopara viticola infections: towards the understanding. Physiological and Molecular Plant Pathology 79:55-63.

Sep 2003 - Dec 2005

Post-Doctoral fellowship. Exploring the nuclear proteome of Medicago truncatula at the switch towards seed filling.

URLEG, Legume Genetics and Ecophysiology Research Unit, INRA of Dijon

A sub-proteomic approach has been developped to identify nuclear proteins at defined stages of seed development in the model legume Medicago truncatula and Pisum sativum, under the supervision of Dr. Karine Gallardo and Dr. Richard Thompson. In order to have nuclei-enriched sub-cellular fractions, I successfully set up a sucrose-based technique of nuclei purification from the very small M. truncatula seeds. Nuclear proteins have being extracted from these fractions, separated by 1DE and their putative identity was characterized by nanoLC-MS/MS, in collaboration with the BIBS Plate-forme of mass spectrometry (INRA of Nantes, France).

Repetto O, Rogniaux H, Colette L and Gallardo K. 2012. The Plant Nucleus Proteome. Mini Review in Frontiers in Plant Proteomics. Front. Plant Sci., 20 December 2012 | doi: 10.3389/fpls.2012.00289.

Repetto O and Gallardo K. 2012. Organelle proteomics of developing seeds: comparison with other plant tissue organelles. In “Seed Development: OMICS Technologies toward Improvement of Seed Quality and Crop Yield. OMICS in Seed Biology" Plant Proteomics: Technologies, Strategies, and Applications”, Agrawal Ganesh K & Rakwal Randeep Eds. Springer Publisher.

Repetto O, Rogniaux H, Firnhaber C, Zuber H, Küster H, Larre C, Thompson R and Gallardo K. 2008. Exploring the nuclear proteome of Medicago truncatula at the switch towards seed filling. The Plant Journal 56:398-410.

Apr 2001 - Sep 2003

Research fellowship. Plant proteomics.

University of Piemonte Orientale ‘A. Avogadro’, Department of Environmental and Life Science

The impact of some highly toxic heavy metals was analysed on the 2DE proteome profile of Cannabis sativa, mycorrhizal or not, and the mitotic cell cycle of Pisum sativum root meristems, under the supervision of Prof. Graziella Berta. During this period, I was also in charge to equipe Biochemistry laboratory of the University with both instruments and reagents for two dimensional electrophoresis.

Nov 1998 - Jun 2003

PhD in Biochemistry, Cellular and Molecular Biology

University of Burgundy, UMR PME-Plant Microbe Environment INRA 1088/CNRS 5184

My PhD thesis has been carried in 'co-tutorship' between Italy and France, at the University of Piemonte Orientale 'A. Avogadro', Department of Environmental and Life Science, Italy and the University of Burgundy, 'UMR Plant Microbe Environment, PME' INRA 1088/CNRS 5184, Dijon, France, under the guidance of Prof. Graziella Berta and Dr. Eliane Dumas-Gaudot, in a multilanguage environment (English, French, Italian). In France, a targeted proteomic approach has been adopted to understand the physiological responses towards an abiotic stress (cadmium, Cd) in pea plants with different sensitivity to Cd, grown in presence or not of the beneficial arbuscular mycorrhizal (AM) fungi. In parallel in Italy, a flow cytometry approach has been used to analyse Cd effects on nuclei populations, mitotic activity and chromosome integrity of pea roots, grown in presence or not of the AM symbiosis. Results from such multidisciplinary approaches have been integrated to get better insight pea responses towards Cd stress and to better understand the role played by AM fungi in Cd-stress alleviation.

Repetto O, Massa N, Gianinazzi-Pearson V, Dumas-Gaudot E, Berta G. 2007. Cadmium effects on populations of root nuclei in two pea genotypes inoculated or not with the arbuscular mycorrhizal fungus Glomus mosseae. Mycorrhiza. 17:111-120.

Fusconi A, Repetto O, Bona E, Massa N, Gallo C, Dumas-Gaudot E, Berta G. 2006. Effects of cadmium on meristem activity and nucleus ploidy in roots of Pisum sativum cv. Frisson seedlings. Environmental and Experimental Botany 58:253-260.

Repetto O, Bestel-Corre G, Dumas-Gaudot E, Berta G, Gianinazzi-Pearson V and Gianinazzi S. 2003. Targeted proteomics to identify cadmium-induced protein modifications in Glomus mosseae-inoculated pea roots. New Phytologist 157:555-567.

Education

Education
Sep 1992 - Mar 1998

Biologist

University of Turin

Bachelor degree in Biology [5 years; speciality: Environment and Ecology]. 110/110 cum laude and dignity of printing. Topics of the 2 years-long experimental thesis: 'Effects of mutualistic and pathosistic symbioses on the structure and activity of root apex in tomato'. Tutor: Prof. G. Berta.

Berta G, Fusconi A, Sampò S, Lingua G, Perticone S, Repetto O. 2000. Polyploidy in tomato roots as affected by arbuscular mycorrhizal colonization. Plant and Soil 226:37-44.

Skills

Skills

Training in Mascot

Training in Mascot. Matrix Science. October 2012. Hannover, Germany.

Training in DIGE and Decyder

Training in DIGE and Decyder. GE Healthcare. June 2012. Munich, Germany.

Interest

Drawing, swimming.