Work History

Work History
Jun 2007 - Present

Postdoctoral Fellow

University of Alabama at Birmingham

Job Duties and Responsibilities:

  • Organized, coordinated and executed project assignments and interpreted results
  • Planned and executed laboratory experiments and technical activities
  • Coordinated successful interdisciplinary collaborations in a team setting
  • Prepared detailed written reports of findings and prepared presentations of the project status


  • Analysis of role of primary cilia in metabolic disorders such as chronic kidney disease
  • Established connection between cellular microtubule dynamics and primary cilia in kidney cells
  • Biomarkers analysis for chronic kidney disease induction and progression
  • Developed new strategies for transfection and stable cell line development for mammalian cells
  • Maintained and generated new transgenic mice colonies to develop novel strategies to study human kidney disease
Aug 2001 - May 2007

Graduate research fellow

University of Georgia

Job Duties and Responsibilities

  • Demonstrate excellent analytical, organizational and multitasking skills
  • Trained undergraduate and graduate students


  • Generated single and double knockout of katanin to study the assembly of motile cilia
  • Executed gene expression studies in Tetrahymena thermophila
  • Established the functional link between katanin and tubulin posttranslational modification         
  • Performed confocal and electron microscopy to demonstrate novel function of katanin


Aug 2001 - May 2007


University of Georgia

Thesis: Significance and Regulation of Microtubule-severing in vivo

Jun 1998 - Aug 2000


Aligarh University

Thesis: Use of domain specific ligands to study urea-induced unfolding of bovine serum albumin

Aug 1995 - May 1998


Delhi University



Computer proficiency

PC/MAC, Adobe Photoshop, MS-Office (Excel, Word, PowerPoint) Endnote, GraphPad Prism.  


Sequence alignment, Blast searching, restriction digest analysis, bioinformatics softwares, DNA and protein sequence analysis  


Confocal Scanning and Spinning Disk, Transmission, Immunoelectron and Scanning electron microscopy, Image analysis (Metamorph, Volocity)  

Cell/Molecular Biology/Biochemistry

Cell and tissue culture, cloning/sub-cloning, vector construction, transient and stable transfection, protein expression and purification, biological assays, ELISA, Mouse and tissue genotyping, Immunohistochemistry, Immunofluorescence, In Situ Hybridization, PCR, RT-PCR, Primer design, SDS-PAGE, Western Blot. Subcellular fractionation  


I am a cell and molecular biologist with more than 10 years of experience in basic research initiatives aimed at better understanding of the cellular mechanisms of human disease. My core competencies are in cytoskeletal analysis in disease models, primary cilia assembly, and chronic kidney disease. My PhD training focused on uncovering an unexpected role of katanin in regulating microtubule dynamics and motile cilia assembly using myriads of advanced molecular biology techniques including, gene expression, generation of single and double knockouts, confocal microscopy and electron microscopy. In my current role, I have developed in vivo and in vitro systems to understand the molecular causes of cystic kidney disease. I am highly proficient in cell culture, cell, molecular and biochemical techniques, confocal microscopy. As needed, I mentored several undergraduate and graduate students and also established productive collaboration with multiple investigators.


Highly motivated scientist with 10 years of experience in cell and molecular biology seeking research scientist position in biotechnology and pharmaceutical industry



  • Sharma N, et al. Soluble levels of cytosolic tubulin regulate ciliary length control. Mol Biol Cell. 2011 Jan 26.
  • Nathan E. Hellman, Yan Liu, David R. Beier, Christopher Ward, Zhaoxia Sun, Neeraj Sharma, Bradley K. Yoder, and Iain A. Drummond. foxj1a regulates cilia function in response to injury and cystic kidney disease. Proc Natl Acad Sci U S A. 2010 Oct 26;107(43):18499-504
  • Dragos Olteanu, Xiafen Liu, Wen Liu,  Venus C. Roper, Neeraj Sharma, Bradley K. Yoder, Lisa M.   Satlin, Erik M. Schwiebert, Mark O. Bevensee. Increased Na+-H+ Exchanger Activity on the Apical Surface of a Cilium-deficient Cortical Collecting Duct Principal Cell Model of Polycystic Kidney Disease (Submitted)
  •  Nicolas F. Berbari, Nicolas W. Kin, Neeraj Sharma, Ed Michaud, John F. Kearney and Bradley K. Yoder. Traf3ip1mutant mice display phenotypes of primary cilia mutants.(Submitted)
  • Neeraj Sharma, et al. Role of primary cilia, proliferation and kidney injury in development of polycystic kidney disease. Manuscript in preparation.
  • Drashti Dave, Neeraj Sharma, Dorota Wloga and Jacek Gaertig. DYF-1 is required for assembly of the axoneme in Tetrahymena. Eukaryotic Cell. 2009 Sep;8(9):1397-406.
  • Dorota Wloga, Krzysztof Rogowski, Neeraj Sharma, Marie-Helene Bré, Nicolette Levilliers, Juliette Van Dijk, Carsten Janke, Bernard Eddé, Kathleen Clark, Martin A. Gorovsky, Maria Jerka-Dziadosz, and Jacek Gaertig. Glutamylation on alpha-tubulin is not essential but affects the assembly and functions of a subset of microtubules in Tetrahymena. Eukaryotic Cell. 2008 Aug;7(8):1362-72
  • Neeraj Sharma, eta al., Ciliary dysfunction in developmental abnormalities and diseases.Invited Review for Book series: Current Topics in Developmental Biology- ‘Ciliary function in mammalian development’. 2008; 85:371-427
  • Neeraj Sharma, et al., Katanin Regulates Dynamics of Microtubules and Assembly of Cilia. The Journal of Cell Biology, (2007) Vol. 178, No. 6, 1065–1079
  • Saad Tayyab, Neeraj Sharma, Mushahid K M. Use of domain specific ligands to study urea- induced unfolding of bovine serum albumin. Biochem. Biophys. Res. Comm. (2000) 277(1): 83-8

    Invited Talks

  • FASEB, Biology of Cilia and Flagella, Annual Meeting, Saxtons River, Vermont (July 2010)

    Conference Presentations

  • Soluble levels of cytosolic tubulin regulate ciliary length control. 50th American Society for Cell Biology, Philadelphia, PA (Dec 2010)
  • Primary cilia and fluid-flow coordinately establish the orientation of mitotic spindle orientation. National Kidney Foundation Spring Clinical Meeting, Orlando, FL (April 2010
  • Temporal analysis of primary cilia in pathogenesis of polycystic kidney disease. 49th American Society for Cell Biology, San Diego, CA (2009)
  • Primary cilia regulate the orientation of mitotic spindles. 48th American Society for Cell Biology, San Francisco, (Dec 2008)
  • Katanin is required for the assembly of motile cilia, 46th American Society for Cell Biology Annual Conference, San Diego, CA (Dec 2006)

  Honors & Awards

  • Travel award, National Kidney Foundation to attend National Kidney Foundation, Clinical Spring meeting. (April 2010)
  • Postdoctoral Research Fellowship: National Kidney Foundation. (2008-2010)
  • Travel award: office of postdoctoral education, University of Alabama at Birmingham to attend American Society for Cell Biology, 2008 annual conference, December (2008)
  • Travel award, 46th American Society for Cell Biology Annual Conference, San Diego, (2006)

  Professional Memberships

  • American Society for Cell Biology
  • Society for Developmental Biology

  Employment Status: Employment authorization for any employer in United States