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Summary

My long-term research goals are to improve maternal and child health by making significant advances in the care of women afflicted by preeclampsia. I have a wide-ranging background in chemical engineering, immunology, molecular, cellular and developmental biology with specific training in immunology and protein-protein interactions. As a masters student, my research focus was on the innate immune response to Salmonella on gut mucosal immunity. As a graduate student at UCSB, I studied the interfacial interaction of marine mussel foot proteins on various surfaces using the surface forces apparatus. In addition to adhesion studies, I studied the biophysical interaction of adhesive plaques, particularly local pH changes within the microenvironment of the mussels foot through a  pH-sensitive surface I developed.

I have experience using the surface forces apparatus (SFA), confocal Microscopy, fluorescent microscopy, scanning electron microscopy, high-pressure reverse phase chromatography (HPLC) and most recently mass cytometry (CyTOF) and Electron Microscopy. As a result, I have the ability to manage multidisciplinary projects and navigate complex research questions.

Education

Jan 2015Present

Postdoctoral Scholar

Stanford University School of Medicine

The objective of my research is to investigate the physiological state of the placenta through monitoring maternal exosomes by the application of Mass Cytometry (CyTOF). By exploiting the sensitivity of this instrument, significant advances in maternal fetal medicine will provide an in depth analysis of the fetal state in vivo, thus allowing safe interrogation of the placenta to ensure healthy pregnancy outcomes.

Research Experience

Stanford University School of Medicine, Stanford, CA

Postdoctoral Scholar, Department of Obstetrics and Gynecology                         2015-present

Project:  Understanding the physiological state of the placenta through monitoring maternal exosomes by the application of Mass Cytometry (CyTOF).

University of California, Santa Barbara, Santa Barbara, CA

Graduate Student; Advisors: Drs. Jacob Israelachvili and J. Herbert Waite         2009 – 2014

Thesis: Wet Adhesion: The Advancement from Mussel Plaques to Applications

California State University, Los Angeles, Los Angeles, CA

Graduate Student; Advisor: Dr. Edith Porter                                                                    2007-2009

Thesis: Expansion of Paneth Cell Population in Response to Enteric Salmonella Infection

University of California, Los Angeles, Los Angeles, CA

Summer internship with the Bridges Program                                                             2003

DNA workshop using various techniques to purify and quantify DNA

Work History

Sep 2009Dec 2014

Teaching Assistant

University of California, Santa Barbara

Biology Laboratory Instructor, Teaching Assistant, MCDB 1AL, UCSB, Fall 2013

  • Provide laboratory instruction to non science majors in general Biology

Medical Immunology, Reader, MCDB 138, UCSB, Spring 2013

  • Graded exams and papers

General Microbiology, Teaching Assistant, MCDB 131, UCSB, Fall 2012

  • Lead discussion sections on various topics in general microbiology

Biology Laboratory Instructor, Biology 1BL - Intro Biology, UCSB, Winter 2010

  • Provide laboratory instruction to non science majors in general Biology
Sep 2007Aug 2009

Teaching/Graduate Assistant

California State Univeristy, Los Angeles

General Immunology, Graduate assistant, Immunology Fall 2009

Animal Biology Laboratory Instructor, Teaching Assistant, Spring 2009

Aug 2008May 2009

Student Advisor

MTPCCR, UCLA

Program designed to encourage students to pursue doctoral programs in the field of cancer control research. Facilitate recruitment to the program and opportunities for mentorship, training, and employment of students. Provide input in all phases of the program. Provide direction on outreach and evaluation. Assist in the recruitment of students. Assist in the recruitment of faculty to present during the MTPCCR summer institute.

Aug 2006Oct 2007

Tutor

Postive Purpose

Served as acting director for three months during the absence of the director. Math, writing, phones and science tutor for pre-school children. Managed workforce, assigned duties and responsibilities, drafted lesson plans, collected tuition and interfaced with the parents regarding their child's progress.

Peer Reviewed Publications


1. Expansion of Paneth cell population in response to enteric Salmonella serovar Typhimurium infection. Martinez Rodriguez NR, Eloi MD, Huynh A, Dominguez T, Lam AH, et al. Infection and immunity. 2012; 80(1):266-75. PubMed [journal] PMID: 22006567 PMCID: PMC3255647
2Polymer Adhesion, Friction, and Lubrication. Hwang D, Wei W, NR, Danner E, Waite J. Zeng H, editor. Online: John Wiley and Sons, Inc.; 2013. A Microcosm of Wet Adhesion: Dissecting Protein Interactions in Mussel Attachment Plaques  [book]
3. Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6.

Nicklisch SC, Das S, NR, Waite JH, Israelachvili JN. Biotechnology progress. 2013; 29(6):1587-93. NIHMSID: NIHMS528382 PubMed [journal] PMID: 24106182 PMCID: PMC3864665

4. A mussel-derived one component adhesive coacervate. Wei W, Tan Y, NR, Yu J, Israelachvili JN, et al. Acta . 2014; 10(4):1663-70. NIHMSID: NIHMS530068 PubMed [journal] PMID: 24060881 PMCID: PMC3960351
5. Intrinsic surface-drying properties of bioadhesive proteins. Akdogan Y, Wei W, Huang KY, Kageyama Y, Danner EW, et al. Angewandte Chemie (International ed. in English). 2014; 53(42):11253-6. NIHMSID: NIHMS633810 PubMed [journal] PMID: 25168789 PMCID: PMC4198389
6. Interfacial pH during mussel adhesive plaque formation. Martinez Rodriguez NR, Das S, Kaufman Y, Israelachvili JN, Waite JH. Biofouling. 2015; 31(2):221-7. NIHMSID: NIHMS678899 PubMed [journal] PMID: 25875963 PMCID: PMC4420479
7.Bridging adhesion of mussel-inspired peptides: of charge, chain length, and surface type. Wei W, Yu J, Gebbie MA, Tan Y, NR, et al.  the ACS journal of surfaces and colloids. 2015; 31(3):1105-12. PubMed [journal] PMID: 25540823 PMCID: PMC4310636
8. Tough coating proteins: subtle sequence variation modulates cohesion. Das S, Miller DR, Kaufman Y, NR, Pallaoro A, et al. Biomacromolecules. 2015; 16(3):1002-8. NIHMSID: NIHMS709145 PubMed [journal] PMID: 25692318 PMCID: PMC4514026
9. Mussel adhesive protein provides matrix for collagen type-1α. Martinez Rodriguez NR, Das S, Kaufman Y, Wei W, Israelachvili JN, et al. Biomaterials. 2015; 51:51-7. NIHMSID: NIHMS660827 PubMed [journal] PMID: 25770997 PMCID: PMC4361793
10. Correction to "Tough Coating Proteins: Subtle Sequence Variation Modulates Cohesion". Das S, Miller DR, Kaufman Y, NR, Pallaoro A, et al.

Biomacromolecules. 2015; 16(7):2254. PubMed [journal]PMID: 26081169

11. High-performance mussel-inspired adhesives of reduced complexity. Ahn BK, Das S, Linstadt R, Kaufman Y, Martinez-Rodriguez NR, et al. Nature communications. 2015; 6:8663. PubMed [journal] PMID: 26478273

* Previous name used: Nadine Martinez Rodriguez

Book Chapters

Dong Soo Hwang, Wei Wei, Nadine R. Rodriguez Martinez, Eric Danner, J. Herbert Waite. (2013). A microcosm of wet adhesion – Dissecting protein interactions in mussel byssal plaques.In Polymer Adhesion, Friction, and Lubrication (pp 319- 349). New York, New York: John Wiley

Fellowship/Honors/Awards

Child Health Research Institue Postdoctoral Award, Child Health Research Institute (CHRI) Fellowship, Stanford University School of Medicine (2015-2016)

  • MCDB Merit Fellowship (August 2014). University of California, Santa Barbara. Awarded to students demonstrating outstanding achievements, creativity, and research progress as a continuing MCDB Ph.D. graduate student.
  • DOW-MRL Travel Fellowship (July 2014). Material Research Laboratory, University of California Santa Barbara. July 2014
  • Outstanding Mentor Award, University of California, Santa Barbara (2013-2014). Presented by the Materials Research Laboratory, UCSB. The MRL Education Programs recognizes graduate students for Outstanding Support of MRL Education Programs.
  • Graduate Research Mentorship Program (GRMP) Fellowship, University of California, Santa Barbara (2013-2014). 
  • Society for Advancing Chicanos/Latin Americans and Native Americans in Science (SACNAS) Conference travel award (2012)
  • UC Systemwide Bioengineering Conference travel award recipient, University of California, Santa Barbara (2012)
  • Chancellor’s Doctoral Incentive Program (CDIP) recipient (2011). 
  • Sally Casanova CSU Pre-Doctoral Scholarship recipient, California State University, Los Angeles (2008). 
  • First place poster presentation, 16th Annual Student Symposium, CSULA (2008)
  • Louis Stokes Alliance for Minority Participation Bridges to the Doctorate program (LSAMP-BD) Fellowship recipient, California State University, Los Angeles (2007-2009). 
  • NIH-supported minority biomedical research scientist (MBRS) – Research Initiative in Science Enhancement (RISE) Fellowship recipient, California State University, Los Angeles (2006-2007). 
  • Beta Beta Beta National Biological Honor Society, California State University, Los Angeles (2005). 

Poster Presentations

  • September 2014 International Workshop in Colloidal and Interfacial Science, Technology, Engineering & Mathematics (CAI-STEM). Research Poster Presentation, "Cohesive collagen type-1α bridges between mussel foot protein films " Nadine R. Martinez Rodriguez, Saurabh Das, Yair Kaufman, Wei Wei, Jacob N. Israelachvili  and J. Herbert Waite. Poster winner.
  • February 2014 Materials Research Outreach Symposium, UCSB. Research poster presentation, "Marine bioinspired dental adhesives". Nadine R. Martinez Rodriguez, Saurabh Das, Yair Kaufman, Wei Wei, Herbert Waite and Jacob Israelachvili
  • February 2013 Materials Research Outreach Symposium, UCSB. Research poster presentation: Surface preparation by marine mussels prior to adhesion. Nadine R. Martinez Rodriguez, Christopher C. Broomel, Jenhwa Heo, Craig J. Hawker, J. Herbert Waite and Jacob N. Israelachvili.
  • February 2012 Materials Research Outreach Symposium, UCSB. Research poster presentation: Mussel plaque attachment mechanism via video confocal microscopy. Nadine R. Martinez Rodriguez, Herbert Waite and Jacob Israelachivili.
  • January 2012, 24th Annual CSU Biotechnology symposium, CSULA. Research poster presentation: Expansion of Paneth Cell Population in Response to Enteric Salmonella Infection. Rodriguez, Nadine, Alexandria Huynh, Naser, Zeina, Desharnais, Robert, Porter, Edith and Nita Salzman.
  • May 2009      American Society for Microbiology (ASM), Philadelphia, PA. Research poster presentation: Response of Paneth Cells in Small Intestine of Mice to Salmonella enterica serovar Typhimurium. Nadine Rodriguez1, Alexandria Huynh 1, Nita Salzman2, and Edith Porter1, 1Department of Biological Sciences, California State University Los Angeles, Los Angeles, CA, 2The Medical College of Wisconsin, Milwaukee, WI
  • February 2009    Host-Pathogen Focus Group, CSULA. Seminar Presentation: Response of Paneth Cells in Small Intestine of Mice to Salmonella enterica serovar Typhimurium
  • February 2009   Post-Bac. And Graduate Students Health Alliance (PBAGSHA), CSULA. Seminar Presentation: Response of Intestinal Stem cells in Small Intestine of Mice to Salmonella enteric serovar Typhimurium
  • April 2008, Biomedical Sciences Seminar Series,Research poster presentation. Nadine Rodriguez, Edith Porte1 and Nita Salzman. Response of Paneth cells in small intestine of mice to Salmonella enterica serovar Typhimurium.
  • February 2008, 16th Annual Student Symposium, CSULA Research poster presentation. Nadine Rodriguez, Edith Porter and Nita Salzman. Response of Paneth cells in small intestine of mice to Salmonella enterica serovar Typhimurium. First Place in Agriculture and Biology division
  • November 2007 Southern California Conference for Undergraduate Research (SCCUR)Research poster presentation. Nadine Rodriguez and Edith Porter. Response of Paneth cells in small intestine of mice to Salmonella enterica serovar Typhimurium

Outreach

Science Writer, Goggles Optional (August 2015 - Present)

Goggles Optional is a podcast where scientists from Stanford University provide their professional yet humorous takes from the world of science. Join us as our hosts explore the significant news and discoveries of the week using a combination of wit, analogies, and words with less than four syllables. Goggles Optional has been featured as a New and Noteworthy science podcast on iTunes and by the Stanford School of Medicine blog. Don’t worry, you don’t need to be a scientist to listen. The Goggles are Optional!  Check it out here: http://gogglesoptional.com

Mentor for Research Experience for Teachers, UCSB (2014)

Project description: Marine mussel plaques are rich in DOPA, a modified tyrosine residue, which is primarily responsible for plaque adhesion. I studied the change in local pH and would like to couple it with cyclic voltametry studies. We would like to study the redox activity of the plaque using a carbon paste electrode over time. Previous studies showed that individual purified proteins are not reservable under oxidation when applied a certain potential using a gold surface electrode. This leads us the hypothesis that the DOPA residues from a complex bond with the gold surface and loses its ability to reduce back to DOPA from DOPA-quinone after oxidation. Using a carbon paste electrode will allow us to study the redox activity without forming bonds with the surface of the electrode. Challenges also include determining a method to measure redox activity as the mussel is secreting the adhesive plaque in real time.  

Mentor for RISE summer undergraduate research program at the Materials Research Laboratory at the University of California, Santa Barbara (2014)

The project will entail studying the mechanics of mussel adhesion on biofilm surfaces and gold sputtered surfaces. We will use a custom tensile machine where will pull adhesive byssal plaques from fouled surfaces using variable speeds and pull angles. The goal of this project is to determine if marine mussels prepare surfaces prior to protein deposition by removing large and small contaminants that may decrease the adhesion at the interface of the surface and byssal plaque. We will compare the results to non-fouled surfaces to gain a better understanding of how mussels attach to various surfaces and how fouled surfaces affect and impact the adhesion of the byssal plaques.

Research Experience for Teachers, UCSB (2012)

Mentor: Nadine R. Martinez Rodriguez Research Project Year: 2012 Research Project Title: Do Adhesive Mussel Plaque Proteins have Antimicrobial Properties? Research Project Description: In the summer of 2012, I assisted PH.D. candidate Nadine R. Martinez Rodriguez on a project to investigate the adhesive plaques of marine mussels. We were primarily working to determine whether the mussels’ adhesive proteins have antimicrobial properties. The mussel foot proteins (Mfps) were isolated using acid/urea polyacrylamide gel electrophoresis (AU-PAGE), size exclusion chromatography (SEC) and reverse phase HPLC (RP-HPLC). These methods purified the proteins down to Mfp-3 and Mfp-6 so that the antimicrobial action of each individual protein could be tested. We tested for the inhibition of growth of E.coli using radial diffusion assay. Preliminary results showed that Mfps 3 and 6 do not inhibit growth of E. coli in radial diffusion assay. However, further tests using different assays and different microbes will be conducted. http://www.mrl.ucsb.edu/education/ret-research-experience-teachers/jessica-thompson

Center for Science and Engineering Partnerships, UCSB (2011)

CNSI Education Programs seek to increase science literacy by engaging the broadest range of learners in science and engineering education opportunities. We collaborate with educational partners at UCSB and in the community, bringing together students, educators, and researchers to develop activities which motivate, recruit and retain students in science and engineering fields. These programs also create new social networks and include mentorship as a particularly powerful resource to engage more students from disadvantaged and/or under-represented backgrounds. http://csep.cnsi.ucsb.edu/miguel-jasso

Kids Do Ecology, UCSB (2011) Visiting Scientist

At the National Center for Ecological Analysis and Synthesis (NCEAS), teams of scientists conduct research on major problems in ecology. NCEAS promotes education and outreach as part of its mission to increase the public understanding of science. The Kids Do Ecology program provides these scientists with an opportunity to volunteer in the Santa Barbara community by adopting local 5th grade classes. Together these scientist-classroom pairs explore specific ecology problems through the collection, analysis and interpretation of experimental data

Girls Incorporated, (2011), Mentor

The day consisted an afternoon of job-shadowing at various corporations around Santa Barbara. The girls will be exposed to a wide range of career opportunities in order to begin to set career goals and learn about paths they need to take to achieve those goals

Early Entrance Program (EEP), California State University, Los Angeles (2009),  Mentor

The Early Entrance Program (EEP) is designed to permit young gifted students to enroll in college as full-time students where the average age of the students is 13.5 years. I mentored a 15-year-old student and trained her to work in our immunology lab. She eventually took over my project after I received my masters and then went on to a Ph.D. program in Immunology at Harvard University. 

Seminars/Panelist

  • “How To Choose The Right Graduate Program”. Invited Guest Panelist. National Science Foundation Louis Stokes Alliance for Minority Participation Bridges to the Doctorate Program (LSAMP-BD). California State University, Los Angeles. November 2015
  • Experience as a Postdoctoral Scholar”. Guest Panelist. Annual African American College Readiness Summit (AACRS) San Jose State University. November 2015.
  • "Life after the PhD program". Invited Guest Panelist. California State University, Los Angeles. March 2015
  • "Experience in a PhD program". Invited Guest Panelist. California State University, Channel Island. October 2014
  • Life of the PhD Student”. Invited Guest Panelist. 3rd Annual California State University, Los Angeles Minority Opportunities in Research (MORE) Programs Research Retreat, California State University Los Angeles. August 2014.
  • Surface preparation by marine mussels prior to adhesion”. Invited Guest Speaker. 3rd Annual Cal State LA MORE Programs Research Retreat, California State University Los Angeles. August 2014.
  • "Life in a PhD program". Invited Guest Panelist. Minority Training in Cancer Control Research (MTPCCR), University of California, Los Angeles. June 2014.
  • Surface preparation by marine mussels prior to adhesion”. Invited Guest Panelist. MORE Programs Seminar at California State University, Los Angeles. May 2013.
  • Applying to Ph.D. Programs”. Invited Guest Panelist. National Science Foundation Louis Stokes Alliance for Minority Participation Bridges to the Doctorate Program (LSAMP-BD). California State University, Los Angeles. March 2013.
  • "Life in a PhD program". Invited Guest Panelist. Minority Training in Cancer Control Research (MTPCCR), University of California, Los Angeles. June 2010.
  • The first year in the Ph.D. program”. Invited Guest Panelist. MORE Panel Discussion at California State University, Los Angeles. July 2010.
  • Applying to grad schools”. Invited Guest Panelist. MORE Panel Discussion at California State University, Los Angeles. July 2009.

References

Available upon request

Skills

Grant writing
Exosome Purification

qEV column, ultra-centrifugatoin, sucrose cushion

Electron Microscopy

Immuno Electron Microscopy

Blood processing
Immunoassays
Western Blot
ELISA
CyTOF
Cell culture
Microbiology techniques:
Culturing and Aseptic Techniques, Enumerating bacteria, identification of pathogen
RT-PCR
Confocal laser Scanning Microscopy, Fluorescent Microscopy, Atomic Force Microscopy, SEM, EDX imaging
• Protein Purification
RP-HPLC, SDS-PAGE, AU-PAGE, Size exclusion chromatography, MALDI
• Quartz crystal microbalance
To study the deposition and coating dynamics of polymers and vesicles on surfaces
Surface Forces Apparatus (SFA)
Interfacial force, rheological and tribological property measurements