Download PDF

Summary

My long-term research goals are to improve maternal and child health by making significant advances in the care of women afflicted by preeclampsia. I have a wide-ranging background in chemical engineering, immunology, molecular, cellular and developmental biology with specific training in immunology and protein-protein interactions. As a masters student, my research focus was on the innate immune response to Salmonella on gut mucosal immunity. As a graduate student at UCSB, I studied the interfacial interaction of marine mussel foot proteins on various surfaces using the surface forces apparatus. In addition to adhesion studies, I studied the biophysical interaction of adhesive plaques, particularly local pH changes within the microenvironment of the mussels foot through a  pH-sensitive surface I developed.

I have experience using the surface forces apparatus (SFA), confocal Microscopy, fluorescent microscopy, scanning electron microscopy, high-pressure reverse phase chromatography (HPLC) and most recently mass cytometry (CyTOF) and Electron Microscopy. As a result, I have the ability to manage multidisciplinary projects and navigate complex research questions.

Education

Stanford University School of Medicine
Jan 2015Present

Postdoctoral Scholar

The objective of my research is to investigate the physiological state of the placenta through monitoring maternal exosomes by the application of Mass Cytometry (CyTOF). By exploiting the sensitivity of this instrument, significant advances in maternal fetal medicine will provide an in depth analysis of the fetal state in vivo, thus allowing safe interrogation of the placenta to ensure healthy pregnancy outcomes.

Research Experience

Stanford University School of Medicine, Stanford, CA

Postdoctoral Scholar, Department of Obstetrics and Gynecology                         2015-Present

Sponsors: Drs. Virginia Winn and Brice Gaudilliere

Project:  Understanding the physiological state of the placenta through monitoring maternal exosomes by the application of Mass Cytometry (CyTOF).

University of California, Santa Barbara, Santa Barbara, CA

Graduate Student; Advisors: Drs. Jacob Israelachvili and J. Herbert Waite         2009 – 2014

Thesis: Wet Adhesion: The Advancement from Mussel Plaques to Applications

California State University, Los Angeles, Los Angeles, CA

Graduate Student; Advisor: Dr. Edith Porter                                                                    2007-2009

Thesis: Expansion of Paneth Cell Population in Response to Enteric Salmonella Infection

University of California, Los Angeles, Los Angeles, CA

Summer internship with the Bridges Program                                                             2003

DNA workshop using various techniques to purify and quantify DNA

Work experience

San Jose State University
2017Present

Visiting lecturer

General Microbiology Lecturer, Fall 2017

  • Provide classroom instruction on basic microbiology concepts to majors and non-majors

Food Microbiology Lecturer, Spring 2018

  • Provide classroom instruction on basic food microbiology concepts to majors and non-majors
University of California, Santa Barbara
Sep 2009Dec 2014

Teaching Assistant

Biology Laboratory Instructor, Teaching Assistant, MCDB 1AL, UCSB, Fall 2013

  • Provide laboratory instruction to non science majors in general Biology

Medical Immunology, Reader, MCDB 138, UCSB, Spring 2013

  • Graded exams and papers

General Microbiology, Teaching Assistant, MCDB 131, UCSB, Fall 2012

  • Lead discussion sections on various topics in general microbiology

Biology Laboratory Instructor, Biology 1BL - Intro Biology, UCSB, Winter 2010

  • Provide laboratory instruction to non science majors in general Biology
California State Univeristy, Los Angeles
Sep 2007Aug 2009

Teaching/Graduate Assistant

General Immunology, Graduate assistant, Immunology Fall 2009

Animal Biology Laboratory Instructor, Teaching Assistant, Spring 2009

MTPCCR, UCLA
Aug 2008May 2009

Student Advisor

Program designed to encourage students to pursue doctoral programs in the field of cancer control research. Facilitate recruitment to the program and opportunities for mentorship, training, and employment of students. Provide input in all phases of the program. Provide direction on outreach and evaluation. Assist in the recruitment of students. Assist in the recruitment of faculty to present during the MTPCCR summer institute.

Postive Purpose
Aug 2006Oct 2007

Tutor

Served as acting director for three months during the absence of the director. Math, writing, phones and science tutor for pre-school children. Managed workforce, assigned duties and responsibilities, drafted lesson plans, collected tuition and interfaced with the parents regarding their child's progress.

Peer Reviewed Publications


1. Martinez Rodriguez NR, Eloi MD, Huynh A, Dominguez T, Lam AH, Carcamo-Molina D, Naser Z, Desharnais R, Salzman NH, Porter E. Expansion of Paneth cell population in response to enteric Salmonella enterica serovar Typhimurium infection. Infection and immunity. 2012; 80(1):266-75. PubMed [journal] PMID: 22006567, PMCID: PMC3255647

2. Polymer Adhesion, Friction, and Lubrication. Hwang D, Wei Wei, Martinez Rodriguez NR, Danner Eric, Waite J. Zeng H, editor. Online: John Wiley and Sons, Inc.; 2013. A Microcosm of Wet Adhesion: Dissecting Protein Interactions in Mussel Attachment Plaques [book] 

3. Nicklisch SC, Das S, Martinez Rodriguez NR, Waite JH, Israelachvili JN. Antioxidant efficacy and adhesion rescue by a recombinant mussel foot protein-6. Biotechnology progress. 2013; 29(6):1587-93. NIHMSID: NIHMS528382 PubMed [journal] PMID: 24106182, PMCID: PMC3864665

4. Wei W, Tan Y, Martinez Rodriguez NR, Yu J, Israelachvili JN, Waite JH. A mussel-derived one component adhesive coacervate. Acta biomaterialia. 2014;10(4):1663-70. NIHMSID: NIHMS530068 PubMed [journal] PMID: 24060881, PMCID: PMC3960351

5. Akdogan Y, Wei W, Huang KY, Kageyama Y, Danner EW, Miller DR, Martinez Rodriguez NR, Waite JH, Han S. Intrinsic surface-drying properties of bioadhesive proteins. Angewandte Chemie (International ed. in English). 2014; 53(42):11253-6. NIHMSID: NIHMS633810 PubMed [journal] PMID: 25168789, PMCID: PMC4198389

6. Martinez Rodriguez NR, Das S, Kaufman Y, Israelachvili JN, Waite JH. Interfacial pH during mussel adhesive plaque formation. Biofouling. 2015; 31(2):221-7.NIHMSID: NIHMS678899 PubMed [journal] PMID: 25875963, PMCID: PMC4420479

7. Wei W, Yu J, Gebbie MA, Tan Y, Martinez Rodriguez NR, Israelachvili JN, Waite JH. Bridging adhesion of mussel-inspired peptides: role of charge, chain length, and surface type. Langmuir : the ACS journal of surfaces and colloids. 2015; 31(3):1105-12. PubMed [journal] PMID: 25540823, PMCID: PMC4310636

8. Das S, Miller DR, Kaufman Y, Martinez Rodriguez NR, Pallaoro A, Harrington MJ, Gylys M, Israelachvili JN, Waite JH. Tough coating proteins: subtle sequence variation modulates cohesion. Biomacromolecules. 2015; 16(3):1002-8. NIHMSID: NIHMS709145 PubMed [journal] PMID: 25692318, PMCID: PMC4514026

9. Martinez Rodriguez NR, Das S, Kaufman Y, Wei W, Israelachvili JN, Waite JH. Mussel adhesive protein provides cohesive matrix for collagen type-1α. Biomaterials. 2015; 51:51-7. NIHMSID: NIHMS660827 PubMed [journal] PMID: 25770997, PMCID: PMC4361793

10. Das S, Miller DR, Kaufman Y, Martinez Rodriguez NR, Pallaoro A, Harrington MJ, Gylys M, Israelachvili JN, Waite JH. Correction to "Tough Coating Proteins: Subtle Sequence Variation Modulates Cohesion". Biomacromolecules. 2015; 16(7):2254. PubMed [journal] PMID: 26081169

11. Peptide Length and Dopa Determine Iron-Mediated Cohesion of Mussel Foot Proteins. Das S, Martinez Rodriguez NR., Wei Wei, Waite J, Israelachvili JN.. Advanced Functional Materials . 2015 August 17; 25(36):5840-5847.  [journal] 

12. Ahn BK, Das S, Linstadt R, Kaufman Y, Martinez-Rodriguez NR, Mirshafian R, Kesselman E, Talmon Y, Lipshutz BH, Israelachvili JN, Waite JH. High-performance  mussel-inspired adhesives of reduced complexity. Nature communications. 2015; 6:8663. PubMed [journal] PMID: 26478273, PMCID: PMC4667698

Previous name: Nadine Martinez Rodriguez

Fellowship/Honors/Awards

  • Institutional Research and Academic Career Development Award (IRACDA)Postdoctoral Fellow, Stanford University School of Medicine - San Jose State University (2016)
  • Child Health Research Institue Postdoctoral Award, Child Health Research Institute (CHRI) Fellowship, Stanford University School of Medicine (2015-2016)
  • MCDB Merit Fellowship (August 2014). University of California, Santa Barbara. Awarded to students demonstrating outstanding achievements, creativity, and research progress as a continuing MCDB Ph.D. graduate student.
  • DOW-MRL Travel Fellowship (July 2014). Material Research Laboratory, University of California Santa Barbara. July 2014
  • Outstanding Mentor Award, University of California, Santa Barbara (2013-2014). Presented by the Materials Research Laboratory, UCSB. The MRL Education Programs recognizes graduate students for Outstanding Support of MRL Education Programs.
  • Graduate Research Mentorship Program (GRMP) Fellowship, University of California, Santa Barbara (2013-2014). 
  • Society for Advancing Chicanos/Latin Americans and Native Americans in Science (SACNAS) Conference travel award (2012)
  • UC Systemwide Bioengineering Conference travel award recipient, University of California, Santa Barbara (2012)
  • Chancellor’s Doctoral Incentive Program (CDIP) recipient (2011). 
  • Sally Casanova CSU Pre-Doctoral Scholarship recipient, California State University, Los Angeles (2008). 
  • First place poster presentation, 16th Annual Student Symposium, CSULA (2008)
  • Louis Stokes Alliance for Minority Participation Bridges to the Doctorate program (LSAMP-BD) Fellowship recipient, California State University, Los Angeles (2007-2009). 
  • NIH-supported minority biomedical research scientist (MBRS) – Research Initiative in Science Enhancement (RISE) Fellowship recipient, California State University, Los Angeles (2006-2007). 
  • Beta Beta Beta National Biological Honor Society, California State University, Los Angeles (2005). 

Poster Presentations

  • September 2014 International Workshop in Colloidal and Interfacial Science, Technology, Engineering & Mathematics (CAI-STEM). Research Poster Presentation, "Cohesive collagen type-1α bridges between mussel foot protein films " Nadine R. Martinez Rodriguez, Saurabh Das, Yair Kaufman, Wei Wei, Jacob N. Israelachvili  and J. Herbert Waite. Poster winner.
  • February 2014 Materials Research Outreach Symposium, UCSB. Research poster presentation, "Marine bioinspired dental adhesives". Nadine R. Martinez Rodriguez, Saurabh Das, Yair Kaufman, Wei Wei, Herbert Waite and Jacob Israelachvili
  • February 2013 Materials Research Outreach Symposium, UCSB. Research poster presentation: Surface preparation by marine mussels prior to adhesion. Nadine R. Martinez Rodriguez, Christopher C. Broomel, Jenhwa Heo, Craig J. Hawker, J. Herbert Waite and Jacob N. Israelachvili.
  • February 2012 Materials Research Outreach Symposium, UCSB. Research poster presentation: Mussel plaque attachment mechanism via video confocal microscopy. Nadine R. Martinez Rodriguez, Herbert Waite and Jacob Israelachivili.
  • January 2012, 24th Annual CSU Biotechnology symposium, CSULA. Research poster presentation: Expansion of Paneth Cell Population in Response to Enteric Salmonella Infection. Rodriguez, Nadine, Alexandria Huynh, Naser, Zeina, Desharnais, Robert, Porter, Edith and Nita Salzman.
  • May 2009      American Society for Microbiology (ASM), Philadelphia, PA. Research poster presentation: Response of Paneth Cells in Small Intestine of Mice to Salmonella enterica serovar Typhimurium. Nadine Rodriguez1, Alexandria Huynh 1, Nita Salzman2, and Edith Porter1, 1Department of Biological Sciences, California State University Los Angeles, Los Angeles, CA, 2The Medical College of Wisconsin, Milwaukee, WI
  • February 2009    Host-Pathogen Focus Group, CSULA. Seminar Presentation: Response of Paneth Cells in Small Intestine of Mice to Salmonella enterica serovar Typhimurium
  • February 2009   Post-Bac. And Graduate Students Health Alliance (PBAGSHA), CSULA. Seminar Presentation: Response of Intestinal Stem cells in Small Intestine of Mice to Salmonella enteric serovar Typhimurium
  • April 2008, Biomedical Sciences Seminar Series,Research poster presentation. Nadine Rodriguez, Edith Porte1 and Nita Salzman. Response of Paneth cells in small intestine of mice to Salmonella enterica serovar Typhimurium.
  • February 2008, 16th Annual Student Symposium, CSULA Research poster presentation. Nadine Rodriguez, Edith Porter and Nita Salzman. Response of Paneth cells in small intestine of mice to Salmonella enterica serovar Typhimurium. First Place in Agriculture and Biology division
  • November 2007 Southern California Conference for Undergraduate Research (SCCUR)Research poster presentation. Nadine Rodriguez and Edith Porter. Response of Paneth cells in small intestine of mice to Salmonella enterica server Typhimurium.
  • Previous name: Nadine Martinez Rodriguez

Outreach

  • Mentor for SIMR - Stanford Institues of Medicine Summer Research Program, Stanford University, (June 2017 - August 2017)
  • Mentor for the RISE (Rising Interest in Science and Engineering) Summer Internship Program, Stanford University  (June 2017 - August) 
  • Science Writer, Goggles Optional, Stanford University (August 2015 - Present)
  • Mentor for Research Experience for Teachers, UCSB (2014)
  • Mentor for RISE summer undergraduate research program at the Materials Research Laboratory at the University of California, Santa Barbara (2014)
  • Research Experience for Teachers, UCSB (2012)
  • Center for Science and Engineering Partnerships, UCSB (2011)
  • Kids Do Ecology, UCSB (2011) Visiting Scientist
  • Girls Incorporated, (2011), Mentor
  • Early Entrance Program (EEP), California State University, Los Angeles (2008 - 2009),  Mentor

Seminars/Panelist

  • How To Choose The Right Graduate Program”. Invited Guest Panelist. National Science Foundation Louis Stokes Alliance for Minority Participation Bridges to the Doctorate Program (LSAMP-BD). California State University, Los Angeles. November 2015
  • Experience as a Postdoctoral Scholar”. Guest Panelist. Annual African American College Readiness Summit (AACRS) San Jose State University. November 2015.
  • "Life after the PhD program". Invited Guest Panelist. California State University, Los Angeles. March 2015
  • "Experience in a PhD program". Invited Guest Panelist. California State University, Channel Island. October 2014
  • Life of the PhD Student”. Invited Guest Panelist. 3rd Annual California State University, Los Angeles Minority Opportunities in Research (MORE) Programs Research Retreat, California State University Los Angeles. August 2014.
  • Surface preparation by marine mussels prior to adhesion”. Invited Guest Speaker. 3rd Annual Cal State LA MORE Programs Research Retreat, California State University Los Angeles. August 2014.
  • "Life in a PhD program". Invited Guest Panelist. Minority Training in Cancer Control Research (MTPCCR), University of California, Los Angeles. June 2014.
  • Surface preparation by marine mussels prior to adhesion”. Invited Guest Panelist. MORE Programs Seminar at California State University, Los Angeles. May 2013.
  • Applying to Ph.D. Programs”. Invited Guest Panelist. National Science Foundation Louis Stokes Alliance for Minority Participation Bridges to the Doctorate Program (LSAMP-BD). California State University, Los Angeles. March 2013.
  • "Life in a PhD program". Invited Guest Panelist. Minority Training in Cancer Control Research (MTPCCR), University of California, Los Angeles. June 2010.
  • The first year in the Ph.D. program”. Invited Guest Panelist. MORE Panel Discussion at California State University, Los Angeles. July 2010.
  • Applying to grad schools”. Invited Guest Panelist. MORE Panel Discussion at California State University, Los Angeles. July 2009.

Skills

Grant writing
Exosome Purification

qEV column, ultra-centrifugatoin, sucrose cushion

Electron Microscopy

Immuno Electron Microscopy

Blood processing

PBMC isolation, plasma and serum processing. 

Immunoassays
Western Blot
ELISA
CyTOF

Mass cytometry, Panel design 

Cell culture
Microbiology techniques:
Culturing and Aseptic Techniques, Enumerating bacteria, identification of pathogen
qRT-PCR
Confocal laser Scanning Microscopy, Fluorescent Microscopy, Atomic Force Microscopy, SEM, EDX imaging
• Protein Purification

RP-HPLC, SDS-PAGE, AU-PAGE, Size exclusion chromatography, MALDI

• Quartz crystal microbalance
To study the deposition and coating dynamics of polymers and vesicles on surfaces
Surface Forces Apparatus (SFA)

Interfacial force, rheological and tribological property measurements

Editor/Reviewer

Guest Editor of Applied Bionics and Biomechanics