Marianne Johnson

Marianne Johnson

Education

Education

Honors and Awards

Medical Scientist Training Program Fellowship, 1989-1995

Spencer T. and Ann W. Olin Fellow, 1993

Phillip Needleman Award, 1995

European Academy of Dermatology Fellowship, 1998

Presentations

Johnson, D.R., Knoll, L.J., Rowley, N., and Gordon, J.I.  Genetic studies of the contribution of S. cervisiae acyCoA Synthetase genes to the regulation of protiens N-myristoylation. Molecular Genetics retreat (1993)

Johnson, D.R., and Gordon, J.I. Genetic analysis of protein N-myristoylation in s.cerevisiae.  Washington University, Division of Biology and Biomedical Sciences, MSTP retreat (1994)

Undergraduate Study

Ohio State Univeristy

Columbus, Ohio

Bachelor of Arts, Chemistry, 1987

Abstracts

Cox, A.D., Johnson, D.R., Solski, P.A., Gordon, J.I., and Buss, J.E.  Incorporation of myristic acid Analogs and myristylated GAG-RAS proteins.  Sixth Annual Meeting on Oncogenes (1990)Johnson, D.R., Knoll, L.J., and Gordon, J.I.  Genetic analysis of protien N-myristoylation in Sacharomyces cerevisiae. Yeast Genetics and Molecular Biology Meeting (1993) 

Knoll, L.J., Johnson, D.R., and Gordon, J.I.  Assessment of S. cerevisiae acylCoA Synthetases effects on protein N-myristoylation using E. coli triple plasmid expression system and invitro assays.  Molecular Parasitology Meeting (1993)

Post-Graduate Training

Medicine Internship,

Department of Internal Medicine,

Barnes Hospital

6/95 - 6/96

Dermatology Residency training,

Division of Dermatology,

Barnes Hospital 

7/96 - 6/99

Scholastic and Professional Experience

Undergraduate Summer Research Program,

Department of Chemistry, Ohio State University

5/80 - 8/80

Honors Chemistry Program

Department of Chemistry, Ohio State University,

Graduated cum laude, 1987

Research Associate

School of Medicine, Ohio State University

8/87 - 5/88

Teaching Assistant, Washington University

Undergraduate Genetics,

2/90 - 5/90

Medical Scientist Training Program,

Washington University School of Medicine,

5/89 - 5/95

Research towards Ph.D. degree,

Lab of Jeffrey L. Gordon,

Department of Molecular Biology and Pharmacology,

Washington University School of Medicine,

5/90 - 5/94

Publications

Johnson, D.R., Cox, A.D., Solski, P.A., Devadas B., Adams, S.P., Leimgruber, R.M., Heuckeroth, R.O., Buss, J.E., and Gordon, J.I.  1990. Functional analysis of protein N-myristoylation: Metabolic labeling studies using three oxygen-substituted analogs of myristic acid and cultured mammalian cells provide evidence for protien-sequence-specific incorporation and analog-specific redistribution.  Proc Natl Acad Sci USA 87:8511-8515

Duronio, R.J., Rudnick, D.A., Johnson, D.R., and Gordon, J.I. 1991.  Myristic acid Auxotrophy caused by mutation of S. cerevisiae myristoyl-CoA: protein N-Myristoyltransferase.  J.Cell Biol  113:1313-1330

Duronio R.J., Rudnick, D.A., Johnson, D.R., and Gordon, J.I.  S. cerevisiae as a model system for studying N-myristoylation of proteins.  In: Lipid Modifications of Proteins, M. Schlesinger, ed., CRC Press, Boca Raton, FL, pp. 21-57 (1992)

Johnson, D.R., Duronio R.J., Langer, C.A., Rudnick, D.A., and Gordon, J.I.  1993.  Genetic and biochemical studies of a mutant Sacchararomyces cerevisiae myristol-CoA: Protein N-myristoyltransferase, nmt72pLeu99->Pro, that produces temperature-sensitive Myristic acid auxotrophy. J Biol Chem 268:483-494

Johnson, D.R., Bhatnagar, R.S., Knoll, L.J., and Gordon, J.I.  1994.  Genetic and Biochemical Studies of Protein N-myristoylation.  Annu Rev Biochem, 63:869-914

Knoll, L.J., Johnson, D.R., Bryant, M.L., and Gordon, J.I.  1995.  Evaluating the functional Signifigance of the myristoyl moiety in N-myristoylproteins.  250:405-435

Johnson, D.R., Knoll, L.J., Rowley, N., Gordon, J.I.  1994. Genetic analysis of the role of Saccharomyces cerevisiae acylCoA synthetase genes in regulating protein N-myristoylation.  J Biol Chem 269:18037-18046

Knoll, L.J., Johnson, D.R., and Gordon, J.I.  1994.  Biomedical studies of three Saccharomyces cerevisiae acylCoA synthetases, Faa1p, Faa2p, Faa3p.  J Biol Chem 269:16384 -16356

Johnson, D.R., Cok, S.J., Feldmann, and Gordon, J.I.  1994.  Suppressors of a conditional allele of the S. cerevisiae myristoyl CoA:protein N-myristoyltransferase gene (nmt-181) reveal proteins involved in regulating N-myristoylation.  Proc Natl Acad Sci USA 91:10158-10162

Knoll, L.J., Johnson, D.R., and Gordon, J.I.  1995. Complementation of Saccharomyces cerevisiae strains containing fatty acid activation gene (FAA) deletions with a mammalian acyCoA synthetase.  J Biol Chem 270:10861-10867