Michael Wilhide

• Finalized research article that was published in Journal of Molecular and Cellular Cardiology (Identification of a NF-κB cardioprotective gene program: NF-κB regulation of Hsp70.1 contributes to cardioprotection after permanent coronary occlusion) • Involved in local job search groups, e.g., the Job Search Focus Group of Hyde Park (Cincinnati) and Job Search Learning Labs-Anderson Township (Cincinnati) • Attend career workshops, seminars, networking events, and career/job fairs • Actively involved with LinkedIn in posting interesting career and science articles/news, active in current discussions, and networking with other members

• Created a marketing plan and enhanced "The Bio-Pharm" LinkedIn group profile and rules, which resulted in a four-fold increase in members in less than two weeks (from 43 to 170+), active discussions and job postings without SPAM This group is for professionals and anyone interested in Biotech, Pharma, Medical/Health Care, and Life Sciences. The mission of the group is to hold active and intelligent discussions centering on diagnostics, medical devices, innovations, clinical trials, research, policy, QA, and current science news. We recommend that our members be actively engaged in posting topics/articles and contributing to the discussions.

(Short-term assignment) • Assisted in analyzing target proteins using HPLC

• Designed and optimized in vivo and in vitro experiments to investigate the role of Nuclear Factor Kappa-B (NF-kB) following myocardial ischemic insults • Executed molecular biology assays to confirm activation of NF-kB following ischemia and determined when NF-kB-dependent cardioprotection occurred • Performed, analyzed, and validated gene expression results that established novel genes involved in the NF-kB dependent cardioprotection and cell death outcomes • Researched and applied scientific literature to employ new knockout mouse models to examine the mechanism of particular genes (e.g. hspa1a and hspa1b) • Identified therapeutic targets more appropriate for clinical development for the treatment of ischemic heart disease • Organized and successfully presented research results in published manuscripts, posters at national research conferences, local seminars, and laboratory meetings • Assisted lab surgeons with in vivo myocardial ischemic models and collaborated with colleagues

• Maintained and prepared various cancer cell cultures • Examined proliferation (PCNA Staining) and apoptotic cell death (TUNEL) on human tumor paraffin sections • Analyzed data and assisted in administrative tasks