Work History

Work History

Research Associate

Dec 2013 - Nov 2015
Karolinska Institutet, Stockholm, Sweden
  • Study of cytotoxicity, genotoxicity and inflammatory effects of organic and inorganic gold-standard nanoparticles using in vitro and ex vivo human cell-culture models.
  • Scientific reporting of findings to European Union Commission, under EU FP7-NanoReg program.
  • ADME study of polymer functionalized and non-functionalized carbon-based nanotubes within normal and diseased animal models with focus on future nanomedicine applications.
  • Teaching nanotoxicology and high-throughput screening in Masters in toxicology program, Karolinska Institutet, Stockholm.
  • Assistant in organizing 'Graduate course in nanotoxicology 2015' at international level and teaching.
  • Co-supervision of Masters and Doctoral program students in their research and thesis works.

Senior Postdoctoral Researcher

Jun 2012 - Nov 2013
Karolinska Institutet, Stockholm, Sweden
  • Study of enzymatic biodegradation of carbon-based nanomaterials by human origin immune cells under in vitro and ex vivo condition with focus on future nanomedicine applications.
  • Teaching nanotoxicology and high-throughput screening in Masters in toxicology program, Karolinska Institutet, Stockholm, Sweden.
  • Co-supervision of Masters and Doctoral program students in their research and thesis works.

Postdoctoral researcher

May 2008 - Oct 2011
Dublin Institute of Technology, Dublin, Republic of Ireland.
  • Study of cytotoxicity, genotoxicity and oxidative stress inducing effects silica-based Zeolite nanoparticles with commercial applications in humans during respiratory and dermal exposures using in vitro human origin cell-culture models.
  • Development of in vitro 3-D biological matrices for rapid Raman spectroscopy screening of mammalian cells.
  • Established high-throughput microcomet assay.
  • Co-supervision of Masters and Doctoral program students in their research and thesis works.

Guest Scientist

Feb 2005 - Jul 2005
University Hospital Essen, Essen, Germany
  • Determination of cytotoxicity, genotoxicity and oxidative stress inducing capacity of asbestos fibers and fine dust particles in human respiratory system using in vitro mammalian cell culture models.
  • Established high-throughput microcomet assay.

Project Assistant

May 2002 - Oct 2004
Indian Institute of Toxicological Research, Lucknow, India
  • Study of asbestos fibers induced cytotoxicity and genotoxicity in ex vivo cultures of human whole blood.
  • Collection of biological samples (nasal lavage and peripheral blood) from controls and asbestos factory workers and screening for inflammation and genotoxicity.

Education

Education

Ph.D. in Toxicology

Sep 2005 - Oct 2008
University of Duisburg-Essen, Essen, Germany

Thesis title: Comparative analysis of fine and nanoparticles for cellular uptake, oxidative stress and genomic damage in human lung cells. Grade: Magna cum laude

M.Sc in Life Sciences

1998 - 2000
University of Lucknow, Lucknow, India

B.Sc in Life Sciences

1995 - 1998
University of Lucknow, Lucknow, India

Skills

Skills

In-vitro cell culture

High-throughput screening

  • Flow-cytometry
  • Luminexanalysis.
  • Microcomet assay .

Molecular biology

  • RT-PCR.
  • Western blotting.
  • SiRNA based analysis.
  • ELISA

Genotoxicity analysis

  • Microcomet assay .
  • OECD 487.
  • OECD 473.
  • OECD 479.

Microscopy

  • Confocal microscopy.
  • Scanning electron microscopy.

Spectroscopy analysis

  • UV-Vis-NIR spectroscopy.
  • Raman spectroscopy.
  • MALDI-tof mass spectroscopy.

Nanometrology

  • Dynamic light scattering-based dynamic particle size distribution and zeta potential analysis.
  • Bruner-Emmett-Teller analysis.
  • Scanning electron microscope and EDX .

Computer-based applications

  • Electronic Lab Notebook (ELN)
  • Graphpad Prism.
  • MS-Office.
  • HTML5.
  • Photoshop.

Memberships

  • Member of United Kingdom registers for Toxicologists.
  • European Registered Toxicologist (ERT).

Awards

  • Research grant from Karolinska Institutet Foundation, 2015.
  • Research grant from Karolinska Institutet Foundation, 2014.
  • Research grant from Karolinska Institutet Foundation, 2013.
  • Best Poster Award, 2nd QNano conference, Czech Republic, 2013.
  • Travel grant from Health Research Board, Ireland for attending 'Molecular mechanism of cancer course' held at National Cancer Institute, Bethesda, United States, 2011.
  • Research grant (UREKA ) from Science Foundation of Ireland, Ireland, 2009.
  • Young Scientist Award for doctoral thesis from University of Duisburg-Essen, Germany, 2009.
  • Travel grant from Deutsche Forschungsgemeinschaft, Germany for attending 47th Society of Toxicology meeting, Seattle, United States, 2008.
  • Best poster award, EnTox conference, Dortmund, Germany, 2007.

List of Publications

https://scholar.google.se/citations?hl=en&user=Nc2j59UAAAAJ

References

Objective