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Itoandon Emolaila Ejiya

PRINCIPAL RESEARCH SCIENTIST

PROFESSIONAL OVERVIEW

I am a research scientist with seven (7) years of laboratory experience. Accomplished in developing and applying new protocols and technologies in the fields of microbiology, microbial engineering, protein engineering, and applied biotechnology. I am an effective communicator with knowledge and interpretation skills of experimental data available to audiences with varied expertise. 

EDUCATION

2014 – 2018, PhD Microbiology (In view), Olabisi Onabanjo University, Ago – Iwoye, Ogun State, Nigeria. Thesis Title: Modelling In vivo modulation of Bacillus and Aspergillus species’ genome for synthesis of optimized extracellular biocatalysts. Advisors: Professor S. A. Bankole and Dr. S. S. Yazdani.

 

Brief Synopsis of Research:

Many researchers would agree that, the main challenge of biocatalysts (enzymes) is stability at extreme conditions. This is also faced with differential variations between laboratory and scale up steps. In this study; in vivo modulatory mechanisms were carried for optimizing functionalities of both Bacillus and Aspergillus spp. for production of amylase and cellulase respectively. Bacillus expression system led to developing a novel Bacillus subtilis sub-specie having optimal growth properties ranging between 37oC – 52oC and pH 5 - 7 while exposure of Aspergillus niger spores to low density of UV-light let to enhancing  growth properties at 60oC and pH 4.0. These improved abilities favoured synthesis of functional stable, high yield biocatalyst under extreme conditions.

2003 - 2005 MSc Microbiology, Olabisi Onabanjo University, Ago – Iwoye, Ogun State, Nigeria.

Thesis Title: Studies on production and activities of amylase, cellulase and pectinase enzymes of Bacillus and Aspergillus species from Stale Fast Foods. Advisor: Professor L. O. Adebajo 

1994 - 1998 BSc Microbiology, Ambrose Ali University, Ekpoma, Edo State, Nigeria.

Thesis Title: Preliminary phytochemical analysis and antimicrobial properties of crude extract of Combretodendron macrocarpum stem bark. Advisor: Professor P. Orhue

Research Interests: My current research interest centres on; •Development and optimization of microbial consortium, •Media optimization, •Strain development, •Improvement of enzyme-substrate mechanisms, •Development of effective fermentation technique.

RESEARCH EXPERIENCE

Jan 2011Present

Senior Research Officer

Federal Institute of Industrial Research (FIIRO), Lagos, Nigeria
  • Production and development of microbial strains for industrial application.
  • Development of microbial expression systems for optimization purpose.
  • Production and scale up of industrial enzymes, metabolites and bioenergy materials.
  • Project development, HACCP and Quality Control Officer
  • Perform testing to support R&D projects in accordance with SOPs and regulations (ISO, GLP).
  • Monitoring and assessing viable samples from a range of sources.
  • Working with computer software to undertake studies and research.
  • Managing and overseeing laboratory activities.
  • Grant application officer.
Sep 2003Aug 2010

Production manager/Microbiologist

Bell - Oaks Industries, Lagos, Nigeria
  • Conduct quality control of finished product for improved market value.
  • Ensuring application of HACCP in production process.
  • Create validation process to ensure consistency in quality of finished goods.
  • Ensuring up to date data compilation of activities between product and end user.
  • Validation of product efficacy.
  • Conduct routine quality control practice.
Oct 2001Jul 2003

Quality Control Officer/Microbiologist

Pharchem Industries Ltd, Lagos, Nigeria
  • Monitoring operations to ensure that they meet production standards.
  • Recommend adjustments to manufacturing processes.
  • Removing all products and materials that fail to meet specifications.
  • Discuss inspection results with those responsible for products.
  • Perform chemical, physical, and microbiological measurements.
  • Interpret test results and assessing their compliance.
  • Identify abnormalities and determine the cause while establishing corrective and preventive actions, and ensuring follow-up.
Aug 1998Aug 1999 

Test Analyst (NYSC - National Youth Sevice Corps)

Freedom Medical Lab, Gusau Zamfara State, Nigeria 
  • Prepare Microbiological growth media and other laboratory solutions to support microbiology laboratory testing.
  • Make detailed observations, documents results and performs data analysis.
  • Maintain patient confidence by keeping laboratory information confidential.

CORE QUALIFICATIONS

Microbiology

Wild type strain isolation, characterization and identification • strain consortia development (bacteria/bacteria, fungal/fungal) • testing strains for microbial properties • strain assay. • competent cells development, • cell compatibility testing.

Bioinformatics

GenBank searching: BLAST, • multiple sequence alignments.

Molecular Biology

DNA isolation, RNA isolation, plasmid and genomic DNA isolation, gene cloning, PCR, Southern blotting, Northern blotting, fluorescent microscopy, primer construction, Omics  research.

Biochemistry

Protein isolation • protein gel electrophoresis • protein precipitation,  •protein purification • protein assay, zymogram.

Cell Culture Media

Media formulation for microbial growth and stability, • optimization of medium, •medium for enzyme and other bioactive compounds extraction, • mixed media formulation.

TRAINING

  • Development of Microbial Expression System. Microbial Engineering Group, International Centre for Genetic Engineering and Biotechnology, New Delhi, Delhi, India. November 1st 2015 – January 10th 2017 
  • Food Biotechnology Training Course for Developing Countries, CAS-TWAS Centre of Excellence for Biotechnology. Beijing, 15th – 19th November, 2014.
  • Grant Writing Workshop. (National Universities Commission, Moore House College, Atlanta and Gregory University, Uturu). March, 10th – 12th, 2014
  • Quality Risk Management in the Food, Beverage, Brewery and Pharmaceutical Industries. (Association of Industrial Microbiologist Society), 18th October, 2013
  • Use of Statistical Soares in Data Analysis and Documentation, 10th - 11th July, 2012 (Nigerian Institute of Food, Science and Technology). 10th – 11th, 2012
  • Hands-on-Training in Bioinformatics for health scientists and technologists. (Nigerian Institute of Medical Research). 13th – 16th , 2011

AWARDS

  • The Arturo Falaschi Fellowship Award, International Centre for Genetic Engineering and Biotechnology (ICGEB). 25TH August, 2015