Dianne Alexis

Dianne Alexis

Work History

Work History
Apr 2007 - Present

Lab Manager

Emory University

·Monitors and review all aspects of technical work as well as quality control and quality assurance.

·Perform general and complex histological procedures, including paraffin embedded and frozen section histology.

·Perform special histological stains, immuno-histochemistry, tissue microarrays and laser capture micro dissection.

·Review and update all laboratory procedures to comply with regulation changes.

·Write protocols for various testing procedures.

·Inventory management and prepare purchase orders for laboratory supplies and reagents.

·Prepare monthly and yearly laboratory statistics and financial reports.

·Evaluate and fine tune testing protocols for new antibodies to detect tissue antigens in both animal and human tissues.

·Trained employees, students and fellow co-workers in all aspects of laboratory operations.

Jan 2007 - Apr 2007

Medical Technologist

Walton Regional Medical Center

·Performed hematological procedures including complete blood cell counts, erythrocyte sedimentation rates, coagulation tests, reticulocyte counts.

·Performed urinalysis, and manual blood cell count differentials.

·Performed general chemistry including immunoassay.

·Received and accessioned specimens from the emergency room and out-patient laboratory.

·Type and screen blood for ABO typing and cross match.

·Set up microbiology plates.

Oct 2006 - Dec 2006


Gwinnett Dermatology

·Assisted with source documentation, data collection.

·Filed documents into regulatory binders.

·Assisted in compiling new patient charts.

·Assisted and participated in Study Closeout.

·Assisted with patient study visits and created maser patient logs.

·Completed case report forms from source documents.

·Participated in Site Initiation and Site Monitoring Visits.

·Participated in the completion of informed consent and assent documents.

Sep 1999 - Apr 2005

Laboratory Supervisor

New York Presbyterian Medical Center

·Performed and applied immuno-histochemical methods based on immuno-enzymatic reactionsusing polyclonal and monoclonal antibodies to detect cell or tissue antigens.

·Experienced with automated and manual immuno-histochemisty, antibody protocol development.

·Supervised and participated in all immune-histochemisty laboratory functions including monitoring and reviewing all technical work.

·Developed and updated standard operating procedures manuals.

·Managed quality assurance, quality improvement and related record keeping and made recommendations for improvement.

·Investigated and resolved lab testing problems.

·Reviewed and updated lab procedures to comply with changes in regulation.

·Performed In Situ Hybridization on Human Papilloma Virus, Epstein Barr Virus, Her2neu.

·Prepared samples for frozen tissues and immune-fluorescence.

·Analyzed and interpreted results using light microscopy.

·Evaluated andfine tuned testing protocols for new antibodies.

·Assisted with research projects and served as a technical resource for Pathologists, Residents, pathology Fellows and less experienced staff.

·Hired and supervised employees.

·Maintained laboratory supplies and equipment.

·Ordered supplies and also responsible for inventory management.

·Participated in the formulation and revision of departmental policies and procedures.

Apr 1997 - Aug 1999

Chief Technologist

Impath Inc.

·Prepared all necessary reagents for staining breast tumor markers and maintain stock reagent solutions.

·Managed the breast prognostic laboratory.

·Performed IHC stains on all breast tumor markers.

·Performed fixation of frozen tissues. Scored tumor cell microscopically.

·Tested and prepared new reagents.

·Performed in-situ hybridization testing for human papilloma virus using Dako autosatiner.

Apr 1996 - Apr 1997

Laboratory Technologist

Kings County Hospital

·Performed hematology – differential and morphological examination of blood smears

·Performed manual procedure such as hemoglobin electrophoresis, HgbA1C, microscopic examination of urine sediments, detection of lupus anticoagulant and manual blood smear.

·Performed ABO blood group typing, RH typing, antibody detection, titration and phenotype.


Sep 2009 - Present


Emory University
Jan 1990 - Dec 1995


Hunter College


Seven years of experience managing a research pathology laboratory.Master’s trained public health professional with a strong interest in cancer research and health disparities.



·Borczuk, A.C., Taub, R.N., Keohan M.L., Hesdorffer M., Hibshoosh, H., Chabot, J.A., Alexis, D., Alsberry, R., Powell C.A:P16 loss and mitotic activity predict poor survival in patients with peritoneal malignant mesothelioma.Departments of Pathology, Medicine, Surgery at Columbia University Mesothelioma center.

·Saqi, A., Alexis, D., Remotti, F., Bhagat, G., Mansukhani, M.: Usefulness of CDX2 and TTF1 in differentiating gastrointestinal from pulmonary carcinoids.AM J Clin Pathology 2005; Vol 123 pp 394 -404.

·Mazziotta R.,Borczuk,A., Alexis D., Mansukhani, M.: Abstract; Differential expression by IHC of CDX2 Transcription Factor in various Adenocarcinomas.

·Lefkowitch, J.H. et al. Acknowledgement: Kupffer Cell Aggregation and Perivenular distribution in steatohepatitis. Mol Path 2002; 15(7) 699-704.


Additional Skills

·Adept in operation of Laser capture micro-dissection, Nanozoomer 2.0HT whole slide scanner, tissue microarray instrument, DAKO auto-stainer, microtome and cryostat.

·Computer skills- Microsoft word, Excel, PowerPoint, knowledge of EpiInfo, making podcasts, customizable start pages, Google docs, technology matrix.


Clinical Research Professional Certificate Program


Gwinnett Technical College, Lawrenceville GA

Supervisory Certificate Program


Columbia University, New York, New York

Certificate Program in Healthcare Management


Brooklyn College, New York

Certificate Program in Molecular Diagnostics 


University of Medicine and Dentistry, New Jersey

Diploma in Clinical Hematology and Transfusion Medicine                                 


New York Blood Center, New York