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Work experience

Jun 2014Present

MBA Business Development Intern

Sequenom
Sep 2012Jun 2014

Postdoctoral Scientist

Univeristy of California-San Diego

I worked on AKAP-mediated cellular signaling and the structural characterization of PKA:PREX1 complexes. I focused on determining the role that PREX1 plays in melanoma metastasis.

Education

Sep 2013Present

Master of Business Administration

Rady School of Management-UC San Diego
Jun 2006Jun 2012

Doctorate

University of California-Irvine

Cumulative GPA: 3.88

Sep 2001Dec 2005

Bachelor of Science

Plymouth State University

Magna cum laude

Cumulative GPA: 3.50 Major GPA: 3.63

References

Philip Collins

Gregory Weiss

Experimental Design

Affiliations

Plymouth State UniversityMulticultural Society– Helped coordinate community outreach events to educate people about various cultures– Learned to effectively communicate with a team comprised of people from diverse backgroundsBoxing club

UC IrvineAssociated Graduate Students (AGS), School of Biological Sciences representativeBusiness and Finance committee, Chairman– Identified business ventures for AGS to pursue by conducting campus-wide electronic surveys to determinepotential market sizes– Helped implement a plan to invest $50,000 of the organization’s savings fund

– While participating in the 2011 Student Lobby Conference, I met with a representative for State Senator Mark Wyland and lobbied for reduced cuts to higher education in California

American Association for the Advancement of Science (AAAS)American Chemical Society (ACS)

Posters & Presentations

American Association for the Advancement of Science National Conference. Single-molecule circuits for investigating real-time binding and enzymatic catalysis. Issa S. Moody, Yongki Choi, Patrick Sims, John Coroneus, Brett Goldsmith, Phil Collins, Gregory Weiss. Vancouver, B.C., February 16-20, 2012

American Association for the Advancement of Science National Conference. Single-molecule circuits for investigating real-time binding and enzymatic catalysis. Issa S. Moody, Yongki Choi, Danny Wan, Richard Jaramillo, Phil Collins, Gregory Weiss. Washington, D.C., February 17-20, 2011

American Association for the Advancement of Science National Conference. Single-molecule circuits for investigating real-time binding and enzymatic catalysis. Issa S. Moody, Danny Wan, Richard Jaramillo, John Coroneus, Brett Goldsmith, Phil Collins, Gregory Weiss. Chicago, IL. February 16-20, 2009

Thesis project specific aims

• Aim 1:Express and purify single-cysteine T4 lysozyme variants for incorporation into nanocircuits •Rationale:Single-cysteine mutants will allow for the site-specific attachment of T4 lysozyme to SWNT sidewalls •Aim 2: Determine how fluctuations in circuit conductance, G(t), correlate to enzymatic catalysis (i.e. binding, turnover) •Rationale: Determining what G(t) fluctuations represent will lay the foundation for incorporating poorly understood proteins into nanocircuits •Aim 3: Use protein-functionalized nanocircuits to investigate the mechanism of caveolin-based disease •Rationale: Caveolin is implicated in several diseases; uncovering how mutant caveolin interacts with binding partners (i.e. PKA) could eventually lead to drug therapies

Online Profiles

Patents

Choi, Y., Moody, I.S., Sims, P.C, Hunt, S.R., Perez, I., Corso, B.L., Weiss, G.A., Collins, P.G. (2011). Biomolecular dynamics monitored by an electronic circuit. U.S. Provisional Patent Application Serial No. 61/539,220

Relevant Documents

Awards, Fellowships & Honors

Rady Postdoctoral Fellowship

NIH Minority Health and Health Disparities International Research Training Grant

NIH Cancer Biology Training Grant (Grant # 5T32CA009054-33)                                                                    

Paroscientific Scholarship Award                      UCI Faculty Mentor Program Fellowship                                                                                                                        UCI Diversity Fellowship                                                                                                                                                    Alliance for Graduate Education and the Professoriate Scholarship                                                                        Plymouth State University, Magna cum Laude                                                                                                              Eagle Scout

Publications

Choi, Y.,† Moody, I.S., Sims, P.C, Hunt, S.R., Perez, I., Weiss, G.A., Collins, P.G. (2012). Single-Molecule Lysozyme Dynamics Monitored by an Electronic Circuit. Science. Vol. 335 pp. 319-324.

Choi, Y., Moody, I.S., Sims, P.C, Hunt, S.R., Corso, B.L., Blaszcazk, L., Collins, P.G, Weiss, G.A.,. (2012). Single-Molecule Dynamics of Lysozyme Processing Distinguishes Linear and Cross-linked Peptidoglycan Substrates. Journal of the American Chemical Society. Vol. 134 pp. 2032-2035.

Moody, I.S., Verde, S.M.,† Overstreet, C.M., Robinson, E., Weiss, G.A.. (2012). In Vitro Evolution of an HIV Integrase Binding Protein from a Library of γS-Crystallin Variants. Bioorganic & Medicinal Chemistry Letters. Vol. 22 pp. 5584-5589.

Moody, I.S., Choi, Y., Olsen, T.J., Sims, P.C., Collins, P.G., Weiss, G.A. (2013). Dissecting Lysozyme by Single-Molecule Techniques. Lysozymes: Sources, Functions and Role in Disease. Nova Science Publishers. ISBN: 978-1-62257-842-9 pp. 193-214.

Yuan, T., Overstreet, C.M, Moody, I.S., Weiss, G.A. (2013) Protein Engineering with Biosynthesized Libraries from Bordetella bronchiseptica Bacteriophage. PLOS ONE. Vol. 8 pp. 1-10.

Choi, Y.,† Olsen, T.J.,† Sims, P.C., Moody, I.S., Hunt, S.R., Corso, B.L., Dang, T., Weiss, G.A., Collins, P.G. (2013). Dissecting Single-Molecule Signal Transduction in Carbon Nanotube Circuits with Protein Engineering. Nano Letters.  Vol. 13 pp. 625-631.

Sims, P.C.; Moody, I.S.; Choi, Y.; Iftikhar, M.; Dong, C.; Corso, B.L.; Gul, O; Collins, P.G.; Weiss, G.A. (2013) Electronic Measurements of the Single-Molecule Processivity of cAMP-Dependent Protein Kinase A. Journal of the American Chemical Society. In press.

† Denotes equal contribution

Summary

My thesis project focused on single-molecule enzymology. Briefly, single enzyme molecules are incorporated into carbon nanotube-based circuits. The goal of the project is to correlate changes in circuit conductance to enzyme activity (i.e., turnover number, enzyme inhibition). Since aberrant protein function causes hundreds of human diseases, determining how such proteins dynamically interact with their environment (e.g.,  binding partners, substrates, etc.) could facilitate the process of drug development. Thus, this technique has potential for contributing to basic as well as translational research.

As a postdoctoral scientist, I worked on AKAP-mediated cellular signaling and the structural characterization of PKA:PREX1 complexes. I focused on determining the role that PREX1 plays in cancer metastasis.