Education

Education
Jan 1992 - Jan 1998

BFSc and MFSc

UNIVERSITY OF AGRICULTURAL SCIENCES


Work History

Work History
Dec 2013 - Present

Research Associate

Mount Sinai Hospital

Development of vaccine candidates against HIV-1 using cytomegalovirus vectors

Dec 2013 - Present

Research Associate

Mount Sinai Hospital, Toronto
*Supervision of Immunology Graduate students research projects *Facilitate scientific collaborations with Kenya AIDS Vaccine Institute and other key partners *Prepare manuscripts for publication * Assist in Clinical Trial protocol preparation * Assist in documentation for Ethics approval for Clinical Trials *Prepare grants for funding *Review external manuscripts and grants Goals attained during this period: 1. Published 1 manuscript in Vaccine (review) as principal author 2. Manuscript submitted as co-author 3. 2 manuscripts in preparation as co-author 4. Obtained approval for Clinical Trial in Nairobi, Kenya
Sep 2012 - Nov 2013

Postdoctoral fellow and Technical Lead

Donnelly Center (CCBR), Toronto

Development of vaccine candidates against HIV-1 using cytomegalovirus vectors

Development of synthetic antibodies against Ebola Virus antigens 

High Throughput Screening – Synthetic phage display screening (96 and 384 well formats), automation techniques

Nov 2009 - Aug 2012

POSTDOCTORAL FELLOW

SUNNYBROOK HEALTH SCIENCES CENTER

Characterization of Glypicans in Renal and Hepatocellular carcinoma

Joseph-Summary

Skills

Skills

Molecular Biology Experiments in Microbiology and Immunology

• Molecular Biology –quantitative real-time PCR of nucleic acids, southern blotting, vector design and construction, Transposon mutagenesis, • Molecular Microbiology: Virological assays, PCR quantification of viral load • High Throughput Screening – phage display screening (96 and 384 well formats), automation techniques • Cell biology, cell viability assays, flow cytometry, stable recombinant cell line generation, recombinant adenovirus, and lentivirus production and transduction, isolation of neural stem cells and skin-derived precursors • Cell Culture - Experience with primary and cancer cell lines, transfection, and electroporation. • Microscopy – Confocal microscopy, Immunohistochemistry. • Protein Biochemistry - Protein expression and purification, ELISA, SDS-PAGE, western blots, native protein gels, immunoprecipitation • Vector Development and Delivery – Intra-cranial delivery of drugs, viral vectors • Mouse models – EAE and demyelination models