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Technical Skills

  • Isolation of DNA/RNA from cells, blood, and tissues
  • Molecular genotyping assay development (qPCR, ddPCR)
  • NGS library construction for whole genome sequencing, targeted resequencing (Nimblegen, IDT xGen), and RNA-Seq (mRNA, total RNA, small RNA). Familiar with both Illumina and PacBio platforms.
  • Bioinformatic pipeline development
  • Programming in MATLAB, Python, R/Bioconductor, CLC Genomic Workbench
  • Cell line engineering (CRISPR-Cas9) 
  • Molecular cloning and cell culturing (E. coli, yeast, and mammalian)
  • FACS assay development (BD AriaII, LSR)
  • Biacore assay development (T100, X100)

Work History



Synthetic Genomics (Mammalian Synthetic Biology)
  • Develop an integrated strategy (CRISPR-Cas9, ddPCR, NGS) for the manipulation of mammalian genomes
  • Develop and optimize a scalable long-range targeted enrichment strategy based on PacBio platform for the characterization of engineered porcine cells
  • Develop and optimize sample (DNA/RNA) extraction and QC protocols for diverse sample types (blood, tissues, synthetic perfusate)
  • Design and perform RNA-Seq experiments for the discovery of novel markers that aid in suppression of immune rejection in xenotransplantation

Scientist, Clinical R&D

Biological Dynamics
  • Developed solid-tumor targeted resequencing panels for the detection of rare mutations in FFPE tissues and cell-free DNA
  • Developed companioned molecular diagnostic assays based on qPCR and ddPCR for the detection of hotspot mutations (EGFR, BRAF, KRAS, PIK3CA)
  • Established and validated clinical protocols for CLIA/CAP certified laboratory

Scientist, Production

Synthetic genomics (SGI-DNA)
  • Optimized scalable oligo assembly strategy for synthesis of large DNA fragments (>10 kb)

Graduate Research Assistant

California Institute of Technology/Stanford University
  • Developed a high-throughput FACS-based screening for engineering of RNA switches
  • Developed a quantitative Biacore-based assay for measuring RNA binding and cleavage activities
  • Supervised graduate and undergraduate students


Apr 2016Present

Statistics for Genomic Data Science

Coursera Course Certificates
Apr 2016Present

Python for Genomic Data Science

Coursera Course Certificates
April 2016Present

Bioconductor for Genomic Data Science

Coursera Course Certificates



PHD, Chemical Engineering

California Institute of Technology

Thesis: High-throughput strategies for the scalable generation of RNA component functions

Advised by Christina Smolke


BS, Chemical Engineering

UC Berkeley

Graduate with high honor; GPA: 3.9/4.0


  • Liang, J.C., Kennedy A.B. and Smolke, .C.D. (2012). A versatile cis-blocking and trans-activation strategy for ribozyme characterization. Nucl Acids Res. doi: 10.1093/nar/gks1036
  • Liang, J.C., Chang A.L., Kennedy A.B. and Smolke, C.D. (2012) A high-throughput, quantitative cell-based screen for efficient tailoring of RNA device activity. Nucl Acids Res. doi: 10.1093/nar/gks636
  • Liang, J.C., Bloom, R.J., and Smolke, C.D. (2011). Engineering biological systems with synthetic RNA molecules. Mol Cell. doi:10.1016/j.molcel.2011.08.023.