Olle Stål, Professor
Phone: +46-(0)10-103 34 91
Fax: +46-(0)10-103 30 90
Department of Clinical and Experimental Medicine
SE-581 85 Linköping
Multidisciplinary scientist, B.Sc in Biochemistry, Ph.D. in Experimental Oncology and Two years international postdoctoral fellowship financed by the Swedish Research Council (VR).
Working in translational medicine, from in vitro models to statistical analysis in breast cancer patient materials. Experienced in molecular biology and cellular assays. Strong computer skills. Proficient in experimental design and troubleshooting. Goal-oriented team player able to collaborate with scientists from different disciplines and nationalities. Experienced in mentoring pre-graduate, master and PhD students. Multilanguage communicator in English, Swedish, Spanish and French. Seeking a collaborative and creative environment within Academia or R&D to apply my skills and extensive experience.
Confocal microscopy, time-lapse microscopy, migration, adhesion, proliferation, Cell death (apoptosis) assays, Flow Cytometry.
Generation and characterization.
Mammalian cell lines.
Transfection, transduction, cloning, RT-PCR, ddPCR, TaqMan arrays, siRNA, Lentivirus.
Protein purification, phosphorylation, ubiquitylation.
Design, and mouse Immunization with synthetic peptides mimicking hormone receptors
Development, sandwich ELISA.
Immunoblotting, cell fractionation, IP, pull-down.
Mentor of pre-graduate and Master students and co supervisor of a PhD student.
GraphPad Prism, Statistica, Image J, Photoshop CS5, Lightroom, Illustrator, Excel, EndNote.
Digital droplet (ddPCR) design, test and standarization using blood, colorectal and breast cancer materials EPH Antibody validation: anti-EPHB2 and EPHB4 using siRNA, immunoblot and IHC. Validation of EPHB and ephrin-B antibodies for IHC in paraffin embedded samples LC-MS/MS analysis and in gel digestion Co-supervisor of a PhD student, master and pre-graduate students Mentor in the medical program.
Designed a TaqMan gene expression array for EPH family: EPH/EFN profile in clinical material Used and interpreted data from Cell Motility array: Explored genes involved in ephrin-B2-mediated cell motility. Explored the clinical value of EPH and ephrins in breast cancer data bases
Studied EphB4-ephrin-B2 downstream signaling pathways in vitro. Investigated EphB4 crosstalk with other receptor tyrosine kinases in breast cancer cells. Generated ephrin-B2 stable cell lines by lentiviral infection and characterized them by immunoblotting, immunofluorescence, cell adhesion, proliferation, migration and transwell assays.
Studied several genetic alterations in the PI3K/AKT/mTOR signaling pathway Explored the clinical value of PKB/AKT expression and activation in breast cancer. Deciphered the impact of PIK3CA mutations and PTEN loss in breast cancer. Found that RPS6K1 and RPS6K2 had divergent roles in breast cancer and gene amplification was associated to therapy response.
Detected HER2 expression in homogenates from breast tumors by Flow Cytometry. Determined S-phase Fraction using the software ModiFit. Prepared frozen sections in the microtome to detect protein expression by immunohistochemistry.
Design and characterization of synthetic peptides mimicking the oestrogen and progesterone receptors Development of ELISA to test the specificity and affinity of monoclonal antibodies recognizing synthetic peptides Development of monoclonal antibodies against estrogen and progesterone receptors Training in different immunization techniques using mice models.
Compared two radiolabeling methods to use 99mTc coupled to a monoclonal anti-EGFR antibody as Radiopharmaceutical agent. Development of the Ascorbate/Dithionite system for direct labeling of the Ior-CEA 1 MAb with 99mTc Development of experimental system to study the role of TGFb in cancer cells
English, French, Spanish, Swedish
Reviewer for Carcinogenesis and Oncotarget and expert reviewer for the Master program at Linköping University
Photography amateur level