Assay development evaluating high through put gene expression assays, Affymetrix Whole Transcript Labeling assay, miRNA profiling, microarray hybridization, DNA and RNA isolation, Bioanalyzer, cDNA synthesis, qPCR, TaqMan primer probe design, molecular cloning, Robotic system.
Antibody-based assays including ELISA, multiplex immunoassays (Luminex and MesoScale), total protein purification and quantitation, Western blotting, beta-galactosidase assays, various cytotoxicity assays, single and multiplex branched DNA (bDNA) technology.
Expert in MS Excel, Prism GraphPad, q-PCR (qBASE, geNorm), BioPlex, MiraiBio Masterplex Luminex data acquiring and analysis, Spotfire, SoftMax Pro, PowerPoint and Photoshop.
Sterile cell culture techniques. Isolate, maintain and separate cell lines from animal tissues and human blood.
ABI 7900 qPCR machine, EnVision microplate reader, Molecular Device M5 microplate reader, Luminex xMAP machines, various liquid handling robots and microplate washers.