Clare Louise Towse

Clare Louise Towse

Work History

Work History
Oct 2010 - present

Senior Fellow

University of Washington

***Returned to University, see Education Section.***

Jun 2004 - Oct 2005

Analyst, Clearasil Skincare New Product Development (NPD)

Boots Healthcare International
Sep 2003 - Jun 2004

Analyst, Pharmaceutical and Analytical Research and Development

AstraZeneca R&D Charnwood
Feb 2002 - Sep 2003

Development Scientist, Formulation Team

Aphton Corporation

Conferences and Communication

ORGANISATION

  • 5th Annual Quantum, Atomic, Molecular and Plasma Physics (QuAMP) conference 2008, assisted academic organiser, Dr June McCombie.
  • Schools' Analyst Competition (Midlands Region) 2005 - 2007, Chief Organiser

INVITED TALKS

  • "A Tale of Two Transition States: Φ-value Restrained Simulations of the NBR1-PB1 Protein Domain", Bioinformatics, Biophysics and Self-Organising Systems Research Seminar, University of Central Lancashire, April 2010

SELECTED ORAL PRESENTATIONS

  • "The Importance of Being Sampled: Unfolding p62-UBA", Physical Chemistry Research Symposium, University of Nottingham, July 2008
  • "Dynamics and Folding of the p62-UBA", CCPB Annual Conference, Bristol, January 2008
  • "Dynamics of the p62-UBA: Correlating simulation with experiment" HPC Conference, University of Nottingham, January 2006

SELECTED POSTER PRESENTATIONS

  • "Dynamics and Unfolding of the p62-UBA Domain: Correlation between Simulations and Experimental data", ANUMOCP Conference, University of Sheffield, June 2008
  • "Dynamics and Unfolding of the p62-UBA Domain: Correlation between Simulations and Experimental data", Biochemical Society Conference - Bringing Together Biomolecular Simulation and Experimental Studies, Manchester Interdisciplinary Centre, September 2007
  • "Dynamics of the p62-UBA Domain", CCPB Annual Conference, University of Nottingham, January 2006
  • "Convection Compensated Diffusion-Ordered Spectroscopy", Royal Society of Chemistry 15th International Meeting on NMR Spectroscopy, Durham University, 2001

Publications

Publications

  • "Molecular Dynamics Simulations", Clare-Louise Towse and Valerie Daggett, Encyclopedia of Biophysics, Protein Folding Volume (in press)
  • “Structural Insights into the Two Sequential Folding Transition States of the PB1 domain of NBR1 from Φ Value Analysis and Biased Molecular Dynamics Simulations” Ping Chen, Clare-Louise Evans (Clare-Louise Towse), Jonathan D. Hirst and Mark S. Searle, Biochemistry, (2010)
  • “Conformation and dynamics of the three-helix bundle UBA domain of p62 from experiment and simulation”, Clare-Louise Evans, Jed E. Long, Thomas R. A. Gallagher, Jonathan D. Hirst, Mark S. Searle, Proteins: Structure, Function and Bioinformatics, 71, 227-40 (2008)
  • “A New Method for Variable Temperature Gradient Shimming”, Clare-Louise Evans, Gareth A. Morris, and Adrian L. Davis, Journal of Magnetic Resonance, 154, 325-328 (2002)

Research Profile

Passionate about proteins and a dynamic research chemist with demonstrated interests in scientific communication and developing a career in both academic research and teaching. I have had postgraduate training in molecular biology, NMR spectroscopy and computational modelling of proteins and while in academia I have published research articles. Having a professional and determined manner, I have been responsible for laboratory management and publishing in-house reports within an industrial setting.

I am intrigued by protein folding in general, but am particularly fascinated by natively unstructured and extremophilic proteins. I am especially passionate about opportunities to use theoretical models to understand or guide experimental studies.

Education

Education
Oct 2005 - May 2010

PhD

The main research themes throughout my PhD revolved around protein structures, their dynamics and folding mechanisms. More specifically, my research focused on the dynamics and folding of proteins found within ubiquitin signalling pathways, concentrating on comparative studies of molecular dynamics (MD) simulations with experimental data for two protein domains, p62-UBA and NBR1-PB1. This was achieved using both all-atom and united-atom force fields with implicit and explicit descriptions of the solvent. Following these comparative equilibrium studies, the unfolding of these two domains were studied using umbrella sampling and Φ-restrained MD simulations. As part of my PhD training, I have experience in basic molecular biology having performed protein expression for production of unlabelled and labelled samples for NMR spectroscopy to obtain comparative data for my simulations.

Oct 2000 - Feb 2002

MPhil

http://www.man.ac.uk

Temperature gradients within an NMR sample can generate convection currents. These currents contribute to phase errors and signal loss in the pulsed field gradient spin echo spectra. An example are the spectra used for diffusion-ordered spectroscopy (DOSY) where such distortion of the spectra leads to an overestimation of the apparent diffusion coefficients. This is particularly noticeable when taking diffusion measurements away from ambient temperature; in mobile solvents such measurements may prove difficult or impossible. The convective motion of spins is coherent, whilst that from diffusion is random. The effects of convection and diffusion can thus be separated by refocusing the dephasing of spins caused by convection. The measured signal attenuation is then solely a consequence of diffusion and relaxation. When I started my MPhil several pulse sequences based on this principle had been described that compensated to varying degrees for the effects of convection; I developed new pulse sequences that offered significantly improved NMR performance while retaining convection compensation. (See Abstracts) 

Gradient shimming methods are now widely used and another example of where convection can have deleterious effects. Typically, these methods employ deuterium spin or gradient echoes to map the magnetic field within the sample, giving rapid and efficient convergence to optimum field homogeneity. Gradient shimming methods are frequently thought to be incompatible with variable temperature operation, although gradient shimming is often successful for modest departures from room temperature. The limiting factor is the loss of echo signal as a result of sample convection, which originates from temperature gradients within the sample. The magnitudes of such gradients depend upon a number of factors, notably probe design, air flow rate, sample viscosity, and operating temperature. I realised and developed with Gareth Morris a new method for variable temperature gradient shimming (see publications); a simple modification was made to the existing gradient shimming pulse sequences that compensated for the effects of convection, resulting in good performance throughout the liquid range of common solvents. 

Skills

Skills

Computer Literacy

Operating Systems Experience: Linux, Mac OS (Darwin), MS-DOS   Scripting Experience: bash, perl, R

NMR Spectroscopy

Molecular Dynamics Simulation

CHARMM, AMBER, NAMD, ilmm

SDS-PAGE

Certifications

Certifications
Oct 2005 - May 2010

PhD, Chemistry

University of Nottingham, UK
Oct 2000 - Feb 2002

MPhil, Chemistry

University of Manchester, UK
Sep 1996 - Jun 2000

MChem (Hons), Chemistry

University of Leicester, UK