Work History

Work History
May 2008 - Present


BioVectra Inc, Canada

  • Executed contract manufacturing projects; project technical evaluation, sourcing raw materials, cell banking & testing, designing scale-down models, execution, trouble shooting and process scale-up
  • Participated in project meetings on updates to the customer
  • Developed up-stream and down stream processes for recombinant therapeutic proteins and successfully scale-up to 500L scale
  • Prepared clinical materials from 500L scale under cGMP guidelines
  • Trouble shoot the processes using scale-down models
  • Authored SOPs, BPRs, CIP & QC documents and Tech transfer documents
  • Participated in process and equipment validations and equipment OQ and PQ
  • Worked in a Team as well lead the Team and Trained technicians
Feb 2008 - Apr 2008

Postdoctoral Researcher

Department of Pharmacy, Dalhousie University, Halifax, Canada
  • Worked in Dr. David Jakeman’s lab in a project; Protein engineering of glycosyltranferases to study glycodiversification of natural bioactive compounds
  • Cloning, expression and purification of glycosyltranferase enzymes (JadS, UrdGT2) and study their substrate specificity towards various sugar nucleotide diphosphates to synthesis Jadomycin analogues
Dec 2004 - Dec 2007

NSERC Visiting Fellow

Institute for Marine Biosciences, NRC, Halifax, NS Canada
  • Worked with Dr. Susan E. Douglas
  • Developed processes (from cloning to purified product) for recombinant therapeutic peptides containing 8 cysteines (fish hepcidins) using bacterial systems
  • Designed, constructed and evaluated various vectors to express peptides
  • Optimized expression, purification and refolding of peptides into active state with correct disulphide bond connectivity
  • Peptides were characterized by MALDI and ESI mass-spectrometry and NMR studies
  • Characterized the peptides, isotope (13C, 15N) labeled the recombinant peptides (pleurocidin) for NMR structural studies
  • Evaluated bacterial periplasmic expression of peptides into native form with co-expression of disulfide bond isomerases
  • Evaluated the therapeutic potential of the peptides using MIC assays
Aug 2003 - Jul 2004

Postdoctoral researcher

KAIST (Korea Advanced Institute of Science and Technology), Taejon, South Korea
  • Worked with Dr. Yong Keun Chang on a project: Genetic modification and process development of Actinosynnema pretiosum for Ansamitocin P-3 production
  • Developed optimized processes for A. pretiosum wild strain and a deletion mutant strain using DOE
Jun 2002 - Jul 2003


Natco Pharma Ltd. Hyderabad, India
  • Involved in project proposals, project planning and execution
  • Developed upstream and downstream processes for biosimilar therapeutic proteins (streptokinase from bacteria and urokinase from mammalian cells)
  • Scaled-up a yeast fermentation process from 5 L to 500L scale
  • Evaluated new synthetic molecules for antimicrobial potential using CLSI methods
  • Collaborated with IICT and University of Hyderabad and coordinated in vivo testing on animal models and in vitro analysis for new synthetic molecules
  • Lead the process development team
  • Authored SOPs, Trained Technicians and mentor the students


1997 - 2002


Andhra University
  • Developed a mutant strain of S. marinensis for high titre neomycin production by classical mutagenic techniques
  • Screened & optimized inexpensive soluble complex medium using DOE to develop a continuous process
  • Evaluated immobilized cells in various reactor configurations (ALR, FBR and PBR)
  • Developed a continuous process with immobilized cells in anAir-lift Reactor (ALR) for neomycin production and operated continuously for 42 days
  • Developed solid state fermentation process for Neomycin production using DOE
  • Participated in isolating a diphenyl sulphone antibiotic producing strain of S. sulfonensis
  • Evaluated natural products and synthetic compounds for antimicrobial activity
1994 - 1995


Andhra University
  • Screened various soil substrates for filamentous bacteria (Actinomycetes) and evaluated for therapeutic and industrial applications
  • Characterized the bacterial isolates using ISP procedure
  • Developed a process for high protease production for one of the isolate
1989 - 1993


Andhra University