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Brungi Preethi Kiran

  •,Housing board,Moula-ali,Hyderabad, Telangana,500040
  • +918019369537, +918019369622 Biotechnology


Completed Msc Biotechnology in 2014 with an aggregate of 72.5%. Recently completed internship  at "E I DUPONT INDIA  PRIVATE LIMITED".



M.Sc Biotechnology - 72.5%

Jawaharlal Nehru Technological University  Hyderabad (JNTUH)

B.Sc Life Sciences - 61.9%

Osmania University

Intermediate - 64.9%

Board of Intermediate Education

Work History

May 2015Nov 2015

Screening of Arabidopsis population for improved traits” at E I DUPONT INDIA LIMITED, DUPONT KNOWLEDGE CENTER, HYDERABAD.

GUIDE: Dr.Bhavna Agashe, Research Scientist, Dupont Knowledge Center.

Cloning and over expression of genes in Arabidopsis and to study the genotypic characters of the transformed plants under different stress conditions.

                      Cloning  of  genes through GATEWAY CLONING TECHNOLOGY and  then mobilizing them in Arabidopsis   plants  by  Agrobacterium mediated transformation   by    Floral Dipping method. Agrobacterium helps in moving  the  gene  of  interest  into  Arabidopsis plant  where the expression of gene allows us to know the effect of  gene upon over expression. These  are further evaluated under different stress conditions to observe the gene response.
May 2015Nov 2015

 EXTRACTION AND PURIFICATION OF CYCLOTIDES FROM    Tridax procumbens                             

GUIDE: Dr. M. LAKSHMI NARASU, Professor, Centre for Biotechnology, IST, JNTUH.


Cyclotides are small cyclic plant proteins with unique cyclized cystine knot (CCK), which enables stable loop formation with increase stability to with stand thermal, chemical and enzymatic degradations. They show a wide range of biological activities like antimicrobial, anti viral, cytotoxic, insecticidal and immune suppressive activity. These characters make them an ideal tool in pharmaceutical drug applications.

        Extraction begins with the harvesting of  plant  materials and using  solvent  systems total proteins  are  collected, then  the total proteins will be analyzed using  FPLC. As the cyclotides exhibit  hydrophobicity  they  elute  in  the retention time  between   20-30 minutes   (i.e. 20-30 retention time eluents will show highest hydrophobicity). The eluted  peaks will be  collected, pooled and further analyzed by SDS-PAGE  to  identify  the  number  of  hydrophobic proteins (10-20 KD size) resent in  fractions  and  then  their  anti-microbial  activity  is  checked  with different  anti-microbial techniques.


Plant tissue culture techniques
  • Preparation of media
  •  Sterilization of explants
  •  Inoculation
  •  Sub culturing and maintenance of explants.
Animal tissue culture techniques
  • Trypsinization 
Microbial techniques
  • Media preparation
  •  Plating and to check anti-microbial activity
  •  Staining of organism
Chromatographic techniques
  • Paper chromatography
  •  TLC
  •  Affinity chromatography
  •  Fast protein Liquid Chromatography
Molecular Biology techniques
  • Isolation and purification of DNA, RNA, Isolation of plasmid vectors
  •  Transformation and screening of recombinant clones.
  •  AGE
  •  PCR
  •  Estimation of proteins
Immunological techniques
  • Ouchterlony technique
  •  Immunoelectrophoresis
  •  Enzyme immunoassay (ELISA)
  •  RIA

Practical Skills

  • Isolation of DNA
  • Estimation of DNA
  • Estimation of proteins using Bradford’s method
  • Isolation of plasmid (alkaline lysis method)
  • Agarose gel electrophoresis
  • Cloning (Gateway method)
  • Chromatographic techniques
  • Microbial techiniques
  • Agrobacterium Transformation
  • PCR

Extra Curricular Activities

Certificates in sports, science fair competition,wall illustration at Inter college competition,National workshop on Advances in Science and Technology and National workshop on Bio-fuels from waste.


Dancing, cooking and listening to music

Personal Dossier

Date of birth: 15/04/1992

Languages known: Telugu,Hindi, English


Dr.Bhyri Priyanka                                         Research scientist, Dupont Knowledge centre, Hyderabad          

Dr. M. Lakshmi Narasu                               Professor, JNTUH, Hyderabad                                                                   

Dr.Bhavna Agashe                                       Research scirntist, Dupont Knowledge centre, Hyderabad