Brungi Preethi Kiran
- H.no.:5-12-183/6/3,Housing board,Moula-ali,Hyderabad, Telangana,500040
- +918019369537, +918019369622
Completed Msc Biotechnology in 2014 with an aggregate of 72.5%. Recently completed internship at "E I DUPONT INDIA PRIVATE LIMITED".
Cloning of genes through GATEWAY CLONING TECHNOLOGY and then mobilizing them in Arabidopsis plants by Agrobacterium mediated transformation by Floral Dipping method. Agrobacterium helps in moving the gene of interest into Arabidopsis plant where the expression of gene allows us to know the effect of gene upon over expression. These are further evaluated under different stress conditions to observe the gene response.
Cloning and over expression of genes in Arabidopsis and to study the genotypic characters of the transformed plants under different stress conditions.
Cyclotides are small cyclic plant proteins with unique cyclized cystine knot (CCK), which enables stable loop formation with increase stability to with stand thermal, chemical and enzymatic degradations. They show a wide range of biological activities like antimicrobial, anti viral, cytotoxic, insecticidal and immune suppressive activity. These characters make them an ideal tool in pharmaceutical drug applications.Extraction begins with the harvesting of plant materials and using solvent systems total proteins are collected, then the total proteins will be analyzed using FPLC. As the cyclotides exhibit hydrophobicity they elute in the retention time between 20-30 minutes (i.e. 20-30 retention time eluents will show highest hydrophobicity). The eluted peaks will be collected, pooled and further analyzed by SDS-PAGE to identify the number of hydrophobic proteins (10-20 KD size) resent in fractions and then their anti-microbial activity is checked with different anti-microbial techniques.
Date of birth: 15/04/1992
Languages known: Telugu,Hindi, English
Dr.Bhyri Priyanka Research scientist, Dupont Knowledge centre, Hyderabad
Dr. M. Lakshmi Narasu Professor, JNTUH, Hyderabad
Dr.Bhavna Agashe Research scirntist, Dupont Knowledge centre, Hyderabad