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Brungi Preethi Kiran

M.sc. Biotechnology

Summary

Completed Msc Biotechnology in 2014 with an aggregate of 72.5%, then  worked as an intern at "E I DUPONT INDIA  PRIVATE LIMITED".  Currently working as a Trainee Scientist in Cytogenetics Department at VIMTA LABS.

Education

20122014

M.Sc Biotechnology - 72.5%

Jawaharlal Nehru Technological University  Hyderabad (JNTUH)
20092012

B.Sc Life Sciences - 61.9%

Osmania University
20072009

Intermediate - 64.9%

Board of Intermediate Education

Work experience

May 2017

Trainee Scientist 

VIMTA LABS LIMITED , CHERLAPALLY

working on Conventional karyotyping 

May 2015Nov 2015

Screening of Arabidopsis population for improved traits” at E I DUPONT INDIA LIMITED, DUPONT KNOWLEDGE CENTER, HYDERABAD.

GUIDE: Dr.Bhavna Agashe, Research Scientist, Dupont Knowledge Center.


ABSTRACT:
Cloning and over expression of genes in Arabidopsis and to study the genotypic characters of the transformed plants under different stress conditions.

                      Cloning  of  genes through GATEWAY CLONING TECHNOLOGY and  then mobilizing them in Arabidopsis   plants  by  Agrobacterium mediated transformation   by    Floral Dipping method. Agrobacterium helps in moving  the  gene  of  interest  into  Arabidopsis plant  where the expression of gene allows us to know the effect of  gene upon over expression. These  are further evaluated under different stress conditions to observe the gene response.
May 2015Nov 2015

 EXTRACTION AND PURIFICATION OF CYCLOTIDES FROM    Tridax procumbens                             

GUIDE: Dr. M. LAKSHMI NARASU, Professor, Centre for Biotechnology, IST, JNTUH.

ABSTRACT:

Cyclotides are small cyclic plant proteins with unique cyclized cystine knot (CCK), which enables stable loop formation with increase stability to with stand thermal, chemical and enzymatic degradations. They show a wide range of biological activities like antimicrobial, anti viral, cytotoxic, insecticidal and immune suppressive activity. These characters make them an ideal tool in pharmaceutical drug applications.

        Extraction begins with the harvesting of  plant  materials and using  solvent  systems total proteins  are  collected, then  the total proteins will be analyzed using  FPLC. As the cyclotides exhibit  hydrophobicity  they  elute  in  the retention time  between   20-30 minutes   (i.e. 20-30 retention time eluents will show highest hydrophobicity). The eluted  peaks will be  collected, pooled and further analyzed by SDS-PAGE  to  identify  the  number  of  hydrophobic proteins (10-20 KD size) resent in  fractions  and  then  their  anti-microbial  activity  is  checked  with different  anti-microbial techniques.

Practical Skills

  • Isolation of DNA
  • Estimation of DNA
  • Estimation of proteins using Bradford’s method
  • Isolation of plasmid (alkaline lysis method)
  • Agarose gel electrophoresis
  • SDS- PAGE
  • Cloning (Gateway method)
  • Chromatographic techniques
  • Microbial techiniques
  • Agrobacterium Transformation
  • PCR
  • Conventional karyotyping 

Extra Curricular Activities

Certificates in sports, science fair competition,wall illustration at Inter college competition,National workshop on Advances in Science and Technology and National workshop on Bio-fuels from waste.

Interests

Dancing, cooking and listening to music

Personal Dossier

Date of birth: 15/04/1992

Languages known: Telugu,Hindi, English

References

Dr.Bhyri Priyanka                                         Research scientist, Dupont Knowledge centre, Hyderabad          
                                                                       
                                                                                    Email: bhyri.priyanka@pioneer.com
                                   

Dr. M. Lakshmi Narasu                               Professor, JNTUH, Hyderabad                                                                   
                                                                   
                                                                                    Email: mangamoori@jntuh.ac.in


Dr.Bhavna Agashe                                       Research scirntist, Dupont Knowledge centre, Hyderabad        
                                                                         
                                                                                  Email: bhavna.agashe@pioneer.com