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About me

In my current position, we work to decipher the novel genomic mechanisms driving neurological disease.  We integrate emerging technologies (such as sequencing, imaging, and informatics) with analyses of post-mortem human brain and CNS disease models to determine the underlying molecular mechanisms driving disease states.  My background in bioengineering and my doctoral work characterizing the molecules involved in regulation of synaptic physiology build the foundation for many of these investigations. 

My goal is to translate fundamental discoveries into treatments which actually improve lives.  To this end, in the past 2 years, I’ve also worked as a senior volunteer analyst with a life science-based venture investment group (Tech Coast Angels) to learn the business components necessary to translate basic knowledge into clinical applications.

Skillsets

Leadership

  • Experience managing diverse sets of scientists and multidisciplinary collaborations for 10+ years
    • Postdoctoral: Led multidisciplinary neurobiology, engineering, and applied physics teams at Scripps, UCSD, Agilent technologies, and Lawrence Livermore National Laboratory
    • Graduate: Led collaborations with NIH, McGill University, Penn State, Yale, Tulane
  • Presenting to diverse audiences
  • Individually applied for and received 3 fellowships
  • Full knowledge and experience of publication process for >13 manuscripts

Neurobiology

  • Synaptic plasticity
  • AMPAR, NMDAR, nAChR
  • Voltage gated ion channels
  • Multielectrode arrays, electrophysiological drug screens in primary culture neurons
  • In depth knowledge of neurodegenerative and neuropsychiatric disease mechanisms
  • In vivo mouse models
  • Single-locus genomic analysis from postmortem human brain

NGS and Informatics

  • Custom data processing and informatic pipelines in Python
  • Wetlab expertise with PacBio, Illumina, Agilent, more
  • Long read sequencing, ChIP-seq, RNA-seq, Single-cell-seq
  • Genomic data mining
  • Diverse experience with a range of command line NGS software toolsets

Molecular and Cellular Biology

  • Comprehensive knowledge and 13+ years of experience with diverse molecular biology toolsets
  • Flow cytometry (neurons)
  • Enzymatic activity and protein binding assays
  • Immunodetection and immunofluorescence
  • Signal transduction
  • Super resolution microscopy

Business

  • Senior volunteer analyst at Tech Coast Angels
  • Competitive, freedom-to-operate, IP analysis
  • Micro-MBA from UCSD Rady School of Executive Development

Professional Experience

Sanford Burnham Prebys Medical Discovery Institute

2017Present

Postdoctoral Research Associate

  • Long read sequencing (PacBio), transcriptomic and genomic
  • Informatic assessments using a range of genomic software tools, analysis pipelines built in python
  • Multielectrode arrays, electrophysiological drug screens in primary culture neurons

Great review of what we're working on from Scientific American: 

https://www.scientificamerican.com/article/scientists-surprised-to-find-no-two-neurons-are-genetically-alike/?WT.mc_id=SA_FB_MB_NEWS

A nice profile from San Diego Life Sciences Network: 

http://lifesciencenetwork.com/blogs/lsn-editorial-team/2017/03/21/stories-from-the-bench-unlocking-the-potential-of-single-cells

Want more information?

Feel free to email: [email protected]

LinkedIn: www.linkedin.com/in/benjamin-siddoway

Tech Coast Angels

2016Present

Senior Analyst

  • Scientific analysis/development of early stage start up companies for the largest angel investment group in the United States.
  • Competitive analysis and due diligence for a series of successfully funded life science start ups

Postdoctoral Research Associate

Dr. Jerold Chun Lab, Dorris Neuroscience Center, La Jolla, California

  • Mosaic genomic variation in cognitive function and neurodegeneration
  • Next generation sequencing (NGS) pipeline creation and informatics
  • Fluorescence-activated cell sorting (FACS) of brain nuclei subpopulations
  • Single-cell (neuronal) sequencing and analyses (genomic/transcriptomic)
  • Single gene fluorescent in situ hybridization (FISH) via super resolution microscopy
  • Awarded individual grants for neurodegenerative disease and genomics work
  • Leadership of multidisciplinary teams at Scripps, UCSD, Agilent technologies, and Lawrence Livermore National Laboratory
  • Presentation and development of initiatives at conferences (Gordon, ASHG, SFN)
  • Leadership in The Scripps Research Institute Society of Fellows and additional San Diego service organizations (see below)

Louisiana State University Health Sciences Center

20052012

PhD Student/Candidate 2006-2011, Postdoctoral Fellow 2012

Dr. Houhui Xia Lab, Neuroscience Center of Excellence, LSUHSC

  • Synaptic Plasticity, ligand gated ion channels (AMPAR, NMDAR, nAChRs), voltage gated (potassium, calcium) ion channels, signaling molecular governing these interactions
  • DNA repair and maintenance enzymes, signaling in the nucleus
  • High throughput phosphoproteomic analysis
  • Identification of neuroprotective properties of a novel compound, patent application process
  • Awarded individual grant for A-T research
  • Managed diverse collaborations with teams at NIH, Tulane, Yale, McGill University
  • Supervision and training for undergraduate, graduate students, postdoctoral fellows
  • Expertise in contingencies and continuity of research projects during and after very significant disruptions (e.g. Hurricane Katrina in New Orleans)
  • More than 10 seminars/presentations at scientific meetings
  • Writing-submission-review-revision process for original peer reviewed publications and review articles (see below)

Louisiana State University Health Sciences Center

20052005

Phd Student

Dr. Rene Anand Lab, Neuroscience Center of Excellence, New Orleans, Louisiana

  • Characterized signaling motif responsible for surface trafficking and expression of nicotinic acetylcholine receptors

Louisiana State University Engineering Department

Undergraduate Researcher

Biological and Agricultural Engineering Department, Baton Rouge, Louisiana

  • Led a team of students in development, initial testing/modeling, and site visit for installation of geothermal/alternative heating and cooling systems at international sites near Adana, Turkey
  • Initial development and early stage US patent application process for remote bovine behavioral monitoring tool
  • Further lab work in bioprocess engineering, nitrogen fixation bioreactors, machine shop construction, biostatistical analysis of smoking cessation, industrial greenhouse design

Publications

Siddoway B, Altimimi H, Hou H, Petralia R, Stellwagen D, Xu B, and Xia H. An Essential Role for Inhibitor-2 Regulated Protein Phosphatase 1 in Synaptic Downscaling. Journal of Neuroscience 2013. 33(27):11206-11.  

Siddoway B, Hou H, Yang H, Petralia R, and Xia H. Synaptic Activity Bidirectionally Regulates a Novel Sequence-Specific S-Q Phosphoproteome in Neurons. Journal of Neurochemistry 2014. 128(6):841-851. [Cover article]

Bushman D, Kaeser G, Siddoway B, Westra J, Rivera R, Rehen S, Yun Y, and Chun J. Genomic mosaicism with increased APP copy number in single neurons from sporadic Alzheimer’s disease brains. eLife. 2015 Feb 4;4. [featured on cover]

Siddoway B*, Hou H, Yang J, Sun L, Yang H, Wang G, Xia H. Potassium channel Kv2.1 is regulated through protein phosphatase-1 in response to increases in synaptic activity. Neuroscience Letters. 2014 Sep 8; 583C:142-147 *Corresponding author

Siddoway B, Hou H, Xia H. Molecular Mechanisms of Homeostatic Downscaling. Neuropharmacology. 2014 3; 78:38-44.

Hou H, Sun L, Siddoway B, Petralia R, Gu H, Yang H, Nairn A, and Xia H. Synaptic NMDA Receptor Stimulation Activates PP1 by Inhibiting its Phosphorylation by CDK5. Journal of Cell Biology 2013. 203(3):521-35

Siddoway B, Hou H, Xia H. Glutamatergic Synapses: Molecular Organization. eLS: Encyclopedia of the Life Sciences 2011 Wiley-Blackwell.

Kaeser G, Siddoway B, Mirendil H, Rohrback S, Chun J. Genomic Disorder and Gene Expression in the Developing Central Nervous System. Encyclopedia of Neuroscience, Oxford Academic Press. 2014

Hou H, Chavez AE, Wang CC, Yang H, Gu H, Siddoway B, Hall BJ, Castillo PE, and Xia H. Siddoway B, Hou H, Xia H. 78:38-44. Journal of Neuroscience 2014. Oct 15;34(42):14006-12

Gao J, Siddoway B, Huang Q, and Xia H. Inactivation of CREB Mediated Gene Transcription by HDAC8 Bound Protein Phosphatase. Biochemical and Biophysical Research Communications 2009 Jan 30; 379(1):1-5.

Education

Louisiana State University Health Sciences Center

20052011

PhD, Molecular and Cellular Neuroscience

PhD Advisor: Houhui Xia, PhD

Committee: Jeffery Erickson, PhD; Youming Lu, PhD; Laura Schrader, PhD; Chunlai Wu, PhD

Louisiana State University

20002005

Biological Engineering

Minor: Psychology

Honors/Awards/Funding

Scripps/UCSD/Salk Lightning Talk Competitions

Top Postdoctoral Presentation of 2016

UCSD Neuroscience

2015

Neuroplasticity of Aging Postdoctoral Fellowship, UCSD Neuroscience

Full salary, +travel and equiptment stipends

The Schaffer Family Research Foundation

2014

Shaffer Foundation Fellowship in Alzheimer’s Research

Full salary, +equiptment stipend

The Ataxia Telangiectasia Children's Project

20122013

Ataxia Telangiectasia [A-T] Children’s Project Postdoctoral Fellowship

[http://atcp.org/page.aspx?pid=3779]

Full salary,

Eli Lilly & Society for Neuroscience

2009

National Graduate Student Travel Award 

Travel Stipend

Society for Neuroscience

2009

Greater New Orleans Society for Neuroscience Award

Travel Stipend

LSUHSC

20052011

LSUHSC Neuroscience Center Graduate Research Assistanceship

Full tuition + stipend

LSU

20002005

Louisiana State University Full Tuition Academic Scholarship

Leadership/Outreach/Service

The Scripps Research Institute:

  • Executive Committee and Networking/Social Chair, TSRI Society of Fellows
  • San Diego science outreach with the Fleet Science Center: Two Scientists Walk into a Bar, Fleet Night of Science, Brushes and beakers: Art and Science
  • Tech Coast Angels Investment Group: Life Sciences and High Tech Start Up Evaluations, Volunteer Analyst
  • Volunteer with San Diego public middle and high schools in science curriculum (science fairs)
  • Scripps/UCSD/Salk Postdoctoral Lighting Talk Monthly Competitions; organizer and presenter

LSUHSC:

  • Vice president and Neuroscience Representative Graduate Student Council
  • Chairman/President, Neuroscience Center Journal Club
  • LSUHSC-New Orleans Public Schools Science Partnership
  • Summer undergraduate neuroscience (SUN) laboratory training program
  • Independent science/medicine workshops in New Orleans public schools
  • SFN Brain Awareness Week at New Orleans Children’s Museum and Library
  • Volunteer and outreach activities in coordination with different relief organizations following hurricane Katrina in New Orleans (e.g. red cross volunteer work, direct construction assistance in flooded neighborhoods with community organizations)

Journal Cover Images

Selected Publication Images

Selected Abstracts

Siddoway B, Rohrback S, Sauvey C, Kaeser G, Chen A, Cerda A, Lee MH, Rivera R, and Chun J.  Genomically mosaic neurons of the adult mouse brain.

2016 Society for Neuroscience

Kaeser K, Siddoway B, Bushman D, and Chun J. Mosaic copy number variation in Alzheimer’s Disease.

2016 Keystone Symposium, Biology of Down’s Syndrome

2014 Society for Neuroscience, Washington D. C.

 

Siddoway B, Altimimi H, Hou H,  Petralia R, Stellwagen D, Xu B, and Xia H.  Regulation of I2-PP1 Complex Function in Neurons and their Critical Role in AMPA Receptor Trafficking and Homeostatic Synaptic Plasticity. [Selected for talk] 

2012 Biennial FASEB Summer Research Conference: Protein Phosphatases, Snowmass, CO

 

Siddoway B, Hou H, Yang J, Sun L, Wang G, and Xia H.  Synaptic NMDA Receptors Regulate Intrinsic Excitability Through Protein Phosphatase 1. 

2012 Protein Phosphatases in Neurobiology: Society for Neuroscience Satellite Conference, New Orleans, LA

 

Protein Phosphatase 1 and Control of Excitability in Neurons: Short Term Plasticity of Intrinsic Potassium Current and Long Term Homeostatic Synaptic Scaling. [Seminar]

2012 LSUHSC Neuroscience Center for Excellence Seminar Series

 

Siddoway B, Hou H, and Xia H.  Activity Induced Dephosphorylation of Kv2.1 Channels by Protein Phosphatase1.

2011 Society for Neuroscience, Washington D.C.

 

Hou H, Siddoway B, Gu H, Yang H, Nairn A, and Xia H.  Regulation of Protein Phosphatase-1 Activity by Synaptic NMDA Receptors: Interplay Among Inhibitor-1, Inhbitor-2, and PP1 Auto-dephosphorylation.

2011 Society for Neuroscience, Washington D.C.

 

Yang H, Siddoway B, and Xia H. Studying the interaction between Protein Phosphatase 1 (PP1) and Binding Proteins by Bioluminescence Resonance Energy Transfer (BRET).

2011Society for Neuroscience, Washington D.C.

  

Regulation of AMPA Receptor Trafficking and Synaptic Architecture by Protein Phosphatase 1 and Endogenous  Regulatory Protein, Inhibitor-2 [Seminar]

2011 LSUHSC Neuroscience Center for Excellence Seminar Series

 

Regulation of Homeostatic Plasticity by Protein Phosphatase 1 Regulatory Protein, Inhibitor-2 [Seminar]

2011 LSUHSC Cell Biology & Anatomy Seminar Series

 

Siddoway B, Hou H, Gao J, Petralia, R, Sheng M, Xu B, and Xia H. Regulation of Homeostatic Plasticity by Protein Phosphatase 1.

2010 Gordon Research Conference: Cell Biology of the Neuron, Cold Spring Harbor, NY

2010 Biennial FASEB Summer Research Conference: Protein Phosphatases, Snowmass, CO

 

Protein Phosphatase 1 Regulation of Synaptic Downscaling [Seminar]

2010 LSUHSC Neuroscience Center for Excellence Seminar Series

 

Protein Phosphatase 1 Regulation of Intrinsic Excitability [Seminar]

2010 LSUHSC Cell Biology & Anatomy Seminar Series

 

Siddoway B, Gao J, Hou H, Dell’Acqua M, and Xia H.  Regulation of Neuronal Protein Phosphatase 1 by Inhibitor-2.

2009 Society for Neuroscience, Chicago, IL

2009 LSUHSC Neuroscience Center Retreat, New Orleans, LA

 

Siddoway B, Gao J, Hou H, and Xia H. Fluorescent/Forster Resonance Energy Transfer (FRET)-Based characterization of the Interaction Between Protein Phosphatase 1 and Inhibitor-2 in Neurons and COS7 Cells.

2009 LSUHSC Graduate Research Symposium, New Orleans, LA

 

A Role for Protein Phosphatase 1 Inhibitor-2 in Homeostatic Plasticity [Seminar]

2009 LSUHSC Neuroscience Center for Excellence Seminar Series

 

Siddoway B, Gao J, Hou H, Dell’Acqua M, and Xia H. Characterization of Protein Phosphatase 1 and Inhibitor-2 Interaction: Regulation Through Different Phosphorylation States, NMDA Receptor Activation, and Different Isoforms of PP1.

2008 Society for Neuroscience Conference, Washington D.C.

2008 LSUHSC Neuroscience Center Retreat, New Orleans, LA

 

Further regular presentations and weekly participation:

Weekly Chun lab meetings, 2013-Present

Faculty/Student Molecular, Cellular Neurobiology Journal Club, 2008-2011

LSUHSC Neuroscience Center Student Journal Club, 2006-2009      

Weekly Xia lab meetings, 2006-2013 

Weekly Xia lab journal club, 2007, 2008, 2012