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Ashley Fletcher

Research Biologist                                             

Objective

Biologist with eight years of academic research experience looking to work in a molecular biology, immunology or biochemistry lab performing advanced experimental procedures and providing support to other lab personnel.

Research Experience

Nov 2011Present

Professional Research Assistant/ Lab Manager

University of Colorado Denver, Denver CO

Division of Pulmonary Sciences and Critical Care Medicine                                                                    

Project: Airway inflammation in the presence of acute lung injury and/or disease                                              

Techniques: Mouse colony maintenance, bronchealveolar lavage, lung tissue harvest, cell culture,    transfection, IHC/IF,  microscopy, DNA and RNA extraction, PCR, RT-qPCR, bacterial culture,  transformations, CRISPR/Cas9

Feb 2010Sep 2011

Professional Research Assistant

University of Colorado Denver, Denver CO

Department of Immunology                                                                                                                      

Project: Relationship between CD79 and B cell anergy                                                                                         Techniques: Mouse colony maintenance, peritoneal lavage, lymphocyte harvest and culture from spleen, bone marrow and lymph nodes; hybridoma culture, protein purification, western blot, flow cytometry, PCR

Sep 2008Jan 2010

Professional Research Assistant

University of Colorado Denver, Denver CO

Department of Radiation Oncology                                                                                                                        

Project:  Effects of chemotherapy drugs on head and neck cancer cells                                                         Techniques: Cell culture, radiation therapy, nuclear extract, clonogenic assay, comet assay, MTT assay, apoptosis assay, IF, microscopy, southern blot, western blot

Jun 2008Sep 2008

Laboratory Technician

University of Kansas Medical Center, Kansas City, KS

Department of Anatomy and Cell Biology                                                                                                              

Project: Create a DNA construct library                                                                                                                  Techniques: Bacterial culture, transformation, DNA extraction (mini-prep), zebrafish husbandry

Jan 2008Mar 2008

Laboratory Technician

DeLaval Manufacturing, Kansas City, MO

Project: QA and R&D on industrial chemical cleaners                                                                                           Techniques: Test efficacy of cleaners, maintain lab equipment, follow FDA, GLP and GDP guidelines

Jun 2007Sep 2007

Biochemistry Intern

UMKC, Kansas City, MO

Assisted the PI in biochemical assays. Maintained equipment and lab organization. Learned many basic molecular biology bench techniques including PCR, gel electrophoresis, bacterial cultures and transformations



Education

Aug 2014Present

Masters of Integrated Sciences

University of Colorado Denver

Currently enrolled in graduate courses as part of the Masters of Integrated Sciences degree focusing on Biochemistry and Molecular Biology. 

Aug 2015

Certificate of Completion

University of Georgia Complex Carbohydrate Research Center

Completed the training course "Separation and Characterization of Glycoprotein and Glycolipid Oligosaccharides"

20042008

Bachelor of Arts

University of Missouri-Kansas City

Graduated with a major in Biology and a minor in Chemistry from the School of Biological Sciences.

20012004

Associate of Arts

Johnson County Community College

Publications

  • Chandler JD, Min E, McElroy CS, ……Fletcher AA (7/13)…… Day BJ. Anti-Inflammatory and Anti-Microbial Effects of Thiocyanate in a Cystic Fibrosis Mouse Model. American Journal of Respiratory Cell and Molecular Biology, 53(2): 193-205, 2015 August
  • Evans CM, Raclawska DS, Ttofali F, Liptzin DR, Fletcher AA, et al. The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nature Communications, 6(6281): Web, 2015 February
  • Hardy IR, Anceriz N, Rousseau F,…..Fletcher A(10/20)……Cambier JC. Anti-CD79 antibody induces B cell anergy that protects against autoimmunity. Journal of Immunology, 192(4):1641-50, 2014 February

  • Roy MG*, Livraghi-Butrico A*, Fletcher AA*, et al. Muc5b is required for airway defence. Nature, 505(7583): 412-6, 2014 January

[*equal contribution authors]


Skills and Qualifications

  • Gene Editing- Use of CRISPR/Cas9 technology to design and generate a knockout mouse, DNA ligation, bacterial transformation and culture. 
  • Molecular Work- Proficient in most molecular biology techniques. These skills include western blots, PCR, qPCR, IHC, DNA and RNA extraction, electrophoresis and immunoprecipitation. 
  • Tissue Culture- Culturing of primary cells (mostly alveolar macrophages) and stable cell lines. Manipulation of cells ex vivo and in vitro; transfections.
  • Animal Work-  Proficient in multiple types of animal procedures and surgeries; survival and non-survival. These include IT, IP, IV, and harvesting of tissues and cells.









References

Dr. David Schwartz

Chair of Medicine

University of Colorado School of Medicine

12700 E 19th Ave

Aurora, CO 80045

[email protected]

303-724-1783


Dr. Ivana Yang

Associate Professor

12700 E 19th Ave

Aurora, CO 80045

[email protected]

303-724-6449


Dr. Christopher Evans

Associate Professor

12700 E 19th Ave

Aurora, CO 80045

[email protected]

303-724-6573