• Denver, CO relocating to Raleigh, NC

Ashley Fletcher

Research Biologist                                             


Biologist with eight years of academic research experience looking to work in a molecular biology, immunology or biochemistry lab performing advanced experimental procedures and providing support to other lab personnel.

Research Experience

Work History

Professional Research Assistant/ Lab Manager

Nov 2011 - Present
University of Colorado Denver, Denver CO

Division of Pulmonary Sciences and Critical Care Medicine                                                                    

Project: Airway inflammation in the presence of acute lung injury and/or disease                                              

Techniques: Mouse colony maintenance, bronchealveolar lavage, lung tissue harvest, cell culture,    transfection, IHC/IF,  microscopy, DNA and RNA extraction, PCR, RT-qPCR, bacterial culture,  transformations, CRISPR/Cas9

Professional Research Assistant

Feb 2010 - Sep 2011
University of Colorado Denver, Denver CO

Department of Immunology                                                                                                                      

Project: Relationship between CD79 and B cell anergy                                                                                         Techniques: Mouse colony maintenance, peritoneal lavage, lymphocyte harvest and culture from spleen, bone marrow and lymph nodes; hybridoma culture, protein purification, western blot, flow cytometry, PCR

Professional Research Assistant

Sep 2008 - Jan 2010
University of Colorado Denver, Denver CO

Department of Radiation Oncology                                                                                                                        

Project:  Effects of chemotherapy drugs on head and neck cancer cells                                                         Techniques: Cell culture, radiation therapy, nuclear extract, clonogenic assay, comet assay, MTT assay, apoptosis assay, IF, microscopy, southern blot, western blot

Laboratory Technician

Jun 2008 - Sep 2008
University of Kansas Medical Center, Kansas City, KS

Department of Anatomy and Cell Biology                                                                                                              

Project: Create a DNA construct library                                                                                                                  Techniques: Bacterial culture, transformation, DNA extraction (mini-prep), zebrafish husbandry

Laboratory Technician

Jan 2008 - Mar 2008
DeLaval Manufacturing, Kansas City, MO

Project: QA and R&D on industrial chemical cleaners                                                                                           Techniques: Test efficacy of cleaners, maintain lab equipment, follow FDA, GLP and GDP guidelines

Biochemistry Intern

Jun 2007 - Sep 2007
UMKC, Kansas City, MO

Assisted the PI in biochemical assays. Maintained equipment and lab organization. Learned many basic molecular biology bench techniques including PCR, gel electrophoresis, bacterial cultures and transformations



Masters of Integrated Sciences

Aug 2014 - Present
University of Colorado Denver

Currently enrolled in graduate courses as part of the Masters of Integrated Sciences degree focusing on Biochemistry and Molecular Biology. 

Certificate of Completion

Aug 2015
University of Georgia Complex Carbohydrate Research Center

Completed the training course "Separation and Characterization of Glycoprotein and Glycolipid Oligosaccharides"

Bachelor of Arts

2004 - 2008
University of Missouri-Kansas City

Graduated with a major in Biology and a minor in Chemistry from the School of Biological Sciences.

Associate of Arts

2001 - 2004
Johnson County Community College


  • Chandler JD, Min E, McElroy CS, ……Fletcher AA (7/13)…… Day BJ. Anti-Inflammatory and Anti-Microbial Effects of Thiocyanate in a Cystic Fibrosis Mouse Model. American Journal of Respiratory Cell and Molecular Biology, 53(2): 193-205, 2015 August
  • Evans CM, Raclawska DS, Ttofali F, Liptzin DR, Fletcher AA, et al. The polymeric mucin Muc5ac is required for allergic airway hyperreactivity. Nature Communications, 6(6281): Web, 2015 February
  • Hardy IR, Anceriz N, Rousseau F,…..Fletcher A(10/20)……Cambier JC. Anti-CD79 antibody induces B cell anergy that protects against autoimmunity. Journal of Immunology, 192(4):1641-50, 2014 February

  • Roy MG*, Livraghi-Butrico A*, Fletcher AA*, et al. Muc5b is required for airway defence. Nature, 505(7583): 412-6, 2014 January

[*equal contribution authors]

Skills and Qualifications

  • Gene Editing- Use of CRISPR/Cas9 technology to design and generate a knockout mouse, DNA ligation, bacterial transformation and culture. 
  • Molecular Work- Proficient in most molecular biology techniques. These skills include western blots, PCR, qPCR, IHC, DNA and RNA extraction, electrophoresis and immunoprecipitation. 
  • Tissue Culture- Culturing of primary cells (mostly alveolar macrophages) and stable cell lines. Manipulation of cells ex vivo and in vitro; transfections.
  • Animal Work-  Proficient in multiple types of animal procedures and surgeries; survival and non-survival. These include IT, IP, IV, and harvesting of tissues and cells.