Download PDF

Summary and Research Interest

Organized Researcher with Ph.D., specialized in Bioanalytical, Immunology, pharmacology, Bio-assay development, small molecule and material characterization, cell-based assays, phytochemistry and genomics with overall 11 years of research experience. Accomplished in generating and applying new protocols/technologies. Well experienced in managing scientists and research teams working to advance industry knowledge. Adept and successful in writing grant proposals and manuscripts.

Research Experience

Ph.D. Doctorate

SASTRA University, TN, INDIA http://sastra.edu/ 

"Standardization and Biochemical evaluation studies on Jatropha tanjorensis J.L. Ellis & Saroja - A Traditional Anti-cancer drug source"

Key working area: Cancer research, Inflammation and Immunomodulation, Bio-analytical. 

  • Characterization of metabolites using UHPLC-MSMS (micrOTOF-Q II, Bruker)
  • LC-MS/MS method development for small molecule and proteomics analysis
  • Proteomic studies through 2D electrophoresis, trypsin in-gel digestion, LC-MSMS and MALDI
  • In silico based binding affinity simulation and QSAR studies using Schrodinger Suite
  • Studying anticancer, immunomodulatory and anti-inflammatory efficacy of various plant drug on cancerous mice/rat model
  • Maintenance of various cell lines (EAC, HUVEC, Vero, A431 & CaCO2)
  • Cell Based Bio-Assay (Human / Mice) to determine the efficacy of phytomolecules
  • Western Blotting Cell Lysate and Cytokine profiling – ELISA for protein studies
  • Also gained hands on experience in characterization techniques such as FT-IR, CHNO analyzer, HPTLC, NMR, AAS, ICP-MS

Industrial Research Experience

Oct 2016Present

Research Scientist

Syngene International Limited

Bangalore, KA, INDIA

In vitro assay based assessment of Pharmacokinetics and Immunogenicity of Therapeutic biomolecules - GLP.

Jul 2015Oct 2016

Senior Research Scientist

OncoStem Diagnostics Pvt Ltd

Bangalore, KA, INDIA

Key Research area: Cancer Biology.

  1. Designed and directed experiments using multiple cell based and molecular techniques.
  2. Processing of Breast cancer FFPE block samples for RNA/DNA extraction.
  3. Performing cDNA conversion and qRT-PCR using SYBR Green and TaqMan assay.
  4. qRT-PCR assay development and validation.
Nov 2014Jun 2015

Scientist-I

Avesthagen Pvt Ltd

Bangalore, KA, INDIA

Key working area: Cancer (Breast) research, Genomics, Biosimilars, Clinical trial database

  1. Establishment of Genomic laboratory: PCR-SSP, RFLP-PCR and gene sequencer
  2. LC-MSMS analysis of metabolites in serum sample
  3. Cell culture facility infrastructure establishment
Apr 2009Aug 2010

Scientist

Biocon Pvt Ltd

Bangalore, KA, INDIA

Key working area: Cancer(Head & Neck/Blood cell/Breast) research, Autoimmunity, Biosimilars.

  1. Separation of Lymphocytes from patients with Autoimmune Disorders specially Rheumatoid Arthritis (RA), Psoriasis (PSO)
  2. Cell surface antigen expression studies (FACS- Beckman Coulter Gallios)
  3. Cell culture maintenance (HUVEC, Melanocyte, Nb2, HUT78, Daudi, SKBr-3, JURKAT, A431)
  4. Cell Based Bio-Assay (Human / Mice) / MLR / ADCC / 7ADD, CFSE
  5. Antibody Binding Assay
  6. Neutralizing antibody Assay
  7. Western Blotting (phospho tyrosine) / Cell Lysate
  8. Cytokine profiling – CBA Flow Cytometer based assay
  9. Anti-lymphocyte antibody Assay (Rosette inhibition/compliment lysis)
Jul 2007Mar 2009

Senior Research Fellow

JIPMER

Puducherry, INDIA

Project worked:

  1. CCRH-MOHWF funded Research Project “Immunological studies in Rheumatoid Arthritis treated with Homeopathic drugs”.
  2. ICMR-INSERM funded Research Project “Studies on T cell vβ repertoire in autoimmuneRheumatic disorder: Correlation with HLA profile”

Trained in Foreign Lab (FRANCE): Spectratyping Gene Length Analysis Technique: Dr. Srini V Kaveri’s, Lab, UMRS 872 (ex 681), INSERM, Paris.

Role Description:-

Separation of Lymphocytes from patients with Autoimmune Disorders (Systemic Lupus Erythromatosis (SLE), Rheumatoid Arthritis (RA), MAS, GBS, Vasculitis, TB).

  • Performing HLA typing.
  • Performing Spectratyping / Immunoscope (Paris, FRANCE).
  • Carrying out Cell culture work.
  • DNA / RNA extraction and cDNA synthesis.
  • Study of Oxidative Stress in autoimmune diseases
Aug 2006Jun 2007

Senior Demonstrator

Christian Medical College and Hospital

Vellore, TN, INDIA

Role Description:-

  1. Class I (A&B) and Class II (DR & DQ) typing. About 2500 typing a year using Microlymphocytoxicity and PCR-SSP (Low & High Resolution) method
  2. Lymphocyte separation and DNA Isolation
  3. HLA B27 correlation studies with autoimmune diseases
  4. Cross matches – Auto, Routine, Extended, Split T & B cell, Monocyte CxM.
  5. Detection of Cold Antibodies.
  6. Coaching students in Various Modern Biochemical and Microbiological Techniques.
Jul 2006Jul 2006

Guest Lecturer

Adhipara Sakthi College of Arts and Science

Vellore, TN, INDIA

Course subject handled: Molecular Biology & Immunology.

Worked as Lab. Instructor and helped students in their academic research projects.

Education

20102015

Ph.D. Doctorate

SASTRA University

Thanjavur, TN, INDIA

Key working area: Cancer research, Inflammation and Immunomodulation, Bio-analytical.

Worked in NABL accredited Laboratory.

Publications

  1. Arun, K.P.and Brindha, P. Jatropha tanjorensis extract induces apoptosis and inhibits tumor in vivo. Journal of Pharmacy and Pharmacology. UNDER REVIEW
  2. Arun, K.P.and Brindha, P. UHPLC–UV–ESI-micrOTOF-Q II analysis of flavonoid fractions from Jatropha tanjorensis. Pharmacognosy Magazine. 10 (39) 472-479 2014
  3. Arun, K.P., Rajesh, S.S. Sankaranarayanan, M.S., Sivaraman, T., Brindha, P. Structural characterizations of lead anti-cancer compounds from the methanolic extract of Jatropha tanjorensis. Bangladesh Journal of Pharmacology. 2014; 9: 452-65
  4. Hasthi, V. Annapurna., Babu, Apoorvaa., Ravichandran, N., Arun, K.P., Brindha, P., Swaminathan, S., Vijayalakshmia, M., Nagarajan, A. Isolation and in silico evaluation of antidiabetic molecules of Cynodon dactylon (L.). Journal of Molecular Graphics and Modelling. 39 (2013) 87–9
  5. Arun, K.P. and Brindha, P. Investigations into the Phenolic and Alkaloid Constituents of Jatropha tanjorensis by LC-MS/MS and Evaluating its Bioactive Property. Asian Journal of Chemistry. 2015; 27(9): 3249-3253
  6. Arun, K.P., Balaji, P., Sivashankari, P.R., David Raj, C., Brindha, P. Proteomics and its Related Biological Activity of Jatropha tanjorensis Ellis & Saroja: An Ethnomedicinal Plant. Asian Journal of Chemistry, Vol. 26, No. 9 (2014).
  7. Balaji, K., Arun, K.P., Brindha, P., Sridharan, K., UmaMaheswari, K., Swaminathan, S., and Rajan, K.S. Bhanupaka: A Green Process in the Preparation of an Indian Ayurvedic Medicine, Lauha Bhasma. Journal of Chemistry. 2013 1-8
  8. Surya, N., Ravichandran, N., Arun, K.P., Brindha, P., Sridharan, K., Rajan, K.S., Uma, K and Swaminathan, S. Botanical standardization studies on Tamarindus indica-bark. Int J Pharm Pharm Sci. Suppl 1, 6 (2014) 70-72
  9. Bino, K., Arun, K.P., Stalin, S., Brindha, P. Stereospermum tetragonam (DL) as an anti-diabetic agent by activating PPARγ and GLUT4. Bangladesh Journal of Pharmacology. 2014; 9: 250-256
  10. Krithika, S.S., Shabir, A.G., Arun, K.P., Vijayalakshmi M. In silico and In vitro evaluation of the anti-inflammatory potential of Centratherum punctatum Cass-A. Journal of biomolecular Structure & Dynamics. 2016
  11. Arun, K.P., Metabolomics – A new approach in plant biotechnology. Research on Biotechnology in India: Some Initiatives and Accomplishments. Oxford & IBH Publishing Co. Pvt. Ltd. International Book Distributing Co. Scientific Publishers (India). BOOK ARTICLE 2016
  12. David, R.C., Arun, K.P., Brindha, P. Gastroprotective potential of hydro-alcoholic extract of Pattanga (Caesalpinia sappan Linn.). Journal of Ethnopharmacology. 2016

Link to Complete list of Articles published:  26
https://scholar.google.co.in/citations?user=_NT2OCMAAAAJ&hl=en