Adriana Masotti

Education

Education
Sep 2008 - Aug 2010

Master of Science

University of Ontario Institute of Technology

Thesis Title: Effects of Soy and Milk Ferments on Measures of Innate Immunity: A Comparison of Effects in Normal and Microgravity Conditions

Work History

Work History

Summer Student

University of Toronto/Mt. Sinai Hospital

❖ Key Achievements ✔  Gained knowledge of normal phase and reverse phase high-performance liquid chromatography (HPLC) technique and concepts ✔  Used SAGE and BLAST for comparative bioinformatics experiments ✔  Utilized HPLC, ELISA and other techniques to quantify, purify and concentrate recombinant tissue kallikrein proteins

May 2008 - Aug 2010

Graduate Research Assistant

University of Ontario Institute of Technology

Key Responsibilities: ✔  Worked with cell culture, including suspension human monocyte/macrophage (U937) and adherent intestinal epithelial (HT-29) cell lines ✔  Bacterial culture in suspension and agar plates of S. thermophilus ST5 and Lb. helveticus R0052, B. longum R0175 ✔  Revived bacterial culture from lyophilized and frozen states ✔  Worked with 3D cell culture using a Rotary Cell Culture System (RCCS) with eukaryotic cells: U937 human monocytic cells, differentiated U937 macrophage like cells, HT-29 human intestinal epithelial cells ✔  Designed an experiment that measured toxicity and immunomodulation due to diverse stimuli in particular to fermented food products using modified protocols ✔  Experienced with laboratory equipment such as Guava Personal Cell Analysis (PCA), BioTek microplate reader, centrifuge, microfuge, cell incubator, biosafety cabinet, autoclave, PCR machine, colony counter, and vacuum filtration ✔  Became adept at protocols for measuring cell surface markers of immunological status, measuring cell viability (both with Guava and trypan blue exclusion), measuring concentration of various cytokines (IL-8, IL-10, TGF-β) with sandwich ELISA, cell viability in response to varied stimuli using a Guava PCA (Personal Cell Analysis) Flow Cytometry System and Guava Viacount assay ✔  Measured cell surface marker expression by immunofluorescence using a Guava PCA and Guava Express assay ✔  Measured immunomodulatory cytokines by immunological methods (ELISA) ✔  Fermented milk and soy preparation with probiotics including lactic acid bacteria such as Lb. helveticus and B. longum ✔  Assisted in purchase of various laboratory consumables, reagents and equipment ✔  Trained new students in current laboratory methods such as 3D cell culture, ELISA, autoclaving procedures, proper disposal of various types of laboratory waste (biological and chemical, wrappers etc), bacterial and cell culture maintenance and growth

Sep 2008 - Apr 2010

Teaching Assistant

University of Ontario Institute of Technology

Key Responsibilies ✔  Provided tutorial sessions to 30 students on a weekly basis which involved explaining fundamental science concepts and laboratory techniques and quizzing them on the material ✔  Supervised students in the laboratory which involved ensuring that student activities were done effectively and safely, troubleshooting when anticipated results were not achieved, demonstrating proper laboratory technique etc.

✔  Supervised 30 students twice a week for 1 to 4 hours on a two week rotating schedule ✔  Delivered tutorial sessions ran between 1 and 2 hours, labs between 3 and 4 hours ✔  Delivered pre-lab and pre-tutorial talks briefing students on material, explained new scientific concepts and made sure they were understood before beginning experiments and assignments and ensured experiments and assignments could be completed independently ✔  Marked, returned, and reviewed assignments weekly ✔  Attended weekly meetings with professors and other teaching assistants to discuss and review labs and tutorials ✔  Made suggestions on changes to current protocols/course material ........................................................................................................................................ ❖ Courses taught: Biology I and II (BIOL 1010U and BIOL 1020U) Biochemistry (BIOL 2040U) Environmental Science (ENVS 1000U)

May 2007 - Apr 2008

Undergraduate Student Research Assistant

University of Ontario Institute of Technology

❖ Key Responsibilities: ✔  Determined antibody levels (IgG, IgM, IgA) in bovine milk samples by ELISA and quantified selected immunomodulatory cytokines (TGF-β1 and TGF-β2) ✔  Measured cell viability via MTT assay ✔  Maintained cultures of adherent cells ✔  General laboratory upkeep

Professional Memberships

Canadian Society of Microbiologists, 2009 - 2011

Canadian Institute of Food Science and Technology, 2010 - 2011

Objective

To secure a technologist/technician position in an organization where full cycle data processing (from collection to interpretation) and experimental research skills would be utilized.

Publications

Effects of soy and dairy ferments on monocyte viability, cytokine production and cell surface molecule expression: impact in a low-shear modeled microgravity system. Acta Astronautica September 24, 2010 Authors: Adriana Masotti M.Sc., Buckley, N.D., Champagne, C.P., Tompkins, T.A., Green-Johnson, J.

Bioactivity of Soy and Milk Ferments on Monocyte and Macrophage Models Food Research International April 1, 2011 Authors: Adriana Masotti M.Sc., Buckley, N.D., Champagne, C.P., Green-Johnson J.

Harnessing functional food strategies for the health challenges of space travel Acta Astronautica 2011 Authors: Adriana Masotti M.Sc., Nicole D. Buckley, Claude P. Champagne, Lisa E. Wagar, Thomas A. Tompkins, Julia M. Green-Johnson

Training Courses and Workshops

Accessibility of Ontarians with Disabilities – Customer Service Standards Training, 2010

Guava PCA User Training, 2009

Radiation Safety, 2008

Laboratory Safety – WHMIS, 2007

Laboratory Safety – Biosafety, 2007

Academic Scholarships and Awards

Canada's Student Participation Program, 2010

ØCanadian Space Agency - $2775

Queen Elizabeth II Aiming for the Top Scholarship, 2004 – 2005

ØGovernment of Canada - $3500

Entrance Scholarship, 2004 – 2005

ØUniversity of Ontario Institute of Technology - $500

Scientific Abstracts and Conference Presentations

Adriana Masotti, Nicole Buckley, Claude Champagne, Thomas Tompkins and Julia Green-Johnson.Effects of soy and dairy ferments on monocyte viability, cytokine production and cell surface molecule expression:  impact in a low-shear modeled microgravity system.Presented at the Space Life Sciences Symposium, Biology in Space, at the 61st International Astronautical Congress, Prague, Czech Republic, October 1st 2010

Adriana I Masotti, Nicole Buckley, Claude Champagne, Julia Green-Johnson.Bioactivity of Probiotic Soy and Milk Ferments on Monocyte and Macrophage Models.Presented at the 49th Canadian Institute of Food Science and Technology/Agriculture and Agri-Food Canada conference, Winnipeg, Canada, May 30th 2010

Nicole D. Buckley, Claude P. Champagne, Adriana I. Masotti, Lisa E. Wagar, Thomas A. Tompkins and Julia M. Green-Johnson.Harnessing functional food strategies for the health challenges of space travel. Presented at the Space Life Sciences Symposium, Applications of Space Medicine to Earth-Related Health Issues, at the 60th International Astronautical Congress, Daejeon, Korea, Oct 13th, 2009

Masotti, A. I., Latos, A.J., Champagne, C.P., Buckley, N.D., Holdway D.A., Green-Johnson, J.M.Immunomodulatory bioactivity of soy and dairy ferments.Presented at the 59th Annual Meeting of the Canadian Society of Microbiologists, Montréal, QC, Canada, June 15th -18th, 2009. Abstract no. 2941

Cooper, W., Masotti A., Wagar L., Champagne C.P., Buckley N.D., Forrester. S.G., Green-Johnson, J.M. Effects of growth phase and substrate on immunomodulatory activity of lactobacilli. Presented at the 96th Annual Meeting of the American Association of Immunologists, Seattle, USA, May 9-12th, 2009.Abstract #1251

Green-Johnson Julia, Johnson Kathy, Masotti Adriana, Wagar Lisa, Fillmore Sherry, Glover Kathleen, MacLaren Leslie, Cathy Enright, Fredeen Alan.Effects of dietary algal supplementation on bovine immunity.Presented at the 96th Annual Meeting of the American Association of Immunologists, Seattle, USA, May 9-12th, 2009.Abstract #2071

Cooper, W., Masotti, A., Wagar, L., Champagne, C.P., Buckley, N., Green-Johnson, J. Immunomodulatory Activity of Lactobacilli strains and Ferments on Macrophage Cytokine Production. Presented at the 5th International conference on Probiotics and Health: Cross talk Now!, Quebec City, QC, Canada, Nov. 6th-7th, 2008.Abstract #SPP008-14

Summary

I am a scientist with over 5 years of hands-on experience and a genuine passion working on cell and tissue culture, cell-based assays, innate immunity, and other biological assays. ........................................................................................................................................ Highlights of Qualifications: I have designed, planned, performed, and evaluated experiments and research projects. I am experienced in the publication of information and data including preparation of papers and supporting graphs and table. I am experienced in the growth, maintenance and storage of bacterial and cell cultures I am experienced with the Full Cycle Data Process including the gathering, evaluating, analyzing, interpreting and summarizing data from experiments. I adapt and recommend new techniques for experiments and projects. I am knowledgeable of safe microbiology laboratory practices and protocols, including cell-based assays. I have presented experiments, collected data, and techniques: Attended and successfully presented data at: CSM 2009 (microbiology), CIFST 2010 (food science) and IAC 2010 (space) I have researched and purchased lab supplies and equipment. I am experienced in light microscopy, cell based assays including ELISA, and basic flow cytometry. I am adept at internet research methods used to search for information relevant to studies including PubMed and various other internet databases.