Download PDF

Birthday / Place

May 1, 1965  /  Bangkok, Thailand

Work History

2011Present

Associate Professor

Faculty of Medicine Siriraj Hospital, Mahidol University

Research and teaching

20022010

Assistant Professor

Faculty of Medicine Siriraj Hospital, Mahidol University
19962001

Instructor

Faculty of Medicine Siriraj Hospital, Mahidol University

Education

19901996

Ph.D. (Pharmacology)

Case Western Reserve University, Cleveland, Ohio, USA

School of Medicine

19821988

M.D.

Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand

Medical degree

19801981

Diploma of high school education

Ministry of Education

Expedite program

19781980

Diploma of secondary school education

Ministry of Education

Expedite program

Skills

Fields of Interested

Adoptive immunotherapy, tumor microenvironment, cytokine-induced killer cells, dendritic cells, pharmacology, tissue engineering, stem cell differentiation, clinical trials

Technical Skills

Flow cytometry, tissue culture, molecular biology, enzymology, high throughput imaging

Research Publications

  1. Wongkajornsilp A, Rosenberry T. Uptake of exogenous sn-1-acyl-2-lyso-phosphatidylinositol into HeLa S3 cells. Reacylation on the cell surface and metabolism to glucosaminyl(acyl)phosphatidylinositol. J Biol Chem 1995; 270: 9147-9153.
  2. Wongkajornsilp A, Luankosolchai R, Huabprasert S, Chanyavanich V, Tisavipat N, Watanapa P. The observation of immunosuppressor(s) derived from cultured tumor cells and its partial neutralization with OK-432. J Thai Med Assoc 2001; 84: 212-222.
  3. Wongkajornsilp A, Sevlever D, Rosenberry TL. Metabolism of exogenous sn-1-alkyl-sn-2-lyso-glucosaminyl-phosphatidylinositol in HeLa D cells: accumulation of glucosaminyl(acyl)phosphatidylinositol in a metabolically inert compartment. Biochem J 2001; 359(Pt 2): 305-13.
  4. Wongkajornsilp A, Tisavipat N, Uiprasertkul M, Chanyavanich V, Sangruchi T, Sangruchi S, Huabprasert S, Chomsamut K, Pan Y, Anthony D. The analysis of peri-tumor necrosis following the subcutaneous implantation of autologous tumor cells transfected with an episome transcribing an antisense insulin-like growth factor 1 RNA in a glioblastoma multiforme subject. J Thai Med Assoc 2001; 84 (Sup. 3): S740-7.
  5. Buntup D, Petmitr S, Wongkajornsilp A, Chanyavanich V, Sangruchi T, Theerapuncharoen V, Kotchabhakdi N, Jutapakdeegul N, Hayashi K, Thangnipon W. Mutation analysis of the PTEN gene localized at chromosome 10q3 in Thai patients with gliomas. ScienceAsia 2002; 28: 417-422.
  6. O-charoenrat P, Wongkajornsilp A, Rhys-Evans PH, Eccles SA. Signaling pathways required for matrix metalloproteinase-9 induction by betacellulin in head-and-neck squamous carcinoma cells. Int J Cancer 2004; 111(2): 174-83.
  7. Wongkajornsilp A, Sangsuriyong S, Hongeng S, Waikakul S, Asavamongkolkul A, Huabprasert S. Effective osteosarcoma cytolysis using cytokine-induced killer cells pre-inoculated with tumor RNA-pulsed dendritic cells. J Orthop Res 2005; 23(6):1460-6.
  8. Vichchatorn P, Wongkajornsilp A, Petvises S, Tangpradabkul S, Pakakasama S, Hongeng S. Dendritic cells pulsed with total tumor RNA for activation NK-like T cells against glioblastoma multiforme. J Neurooncol. 2005; 75(2): 111-8.
  9. Panichakul T, Intachote P, Wongkajornsilp A, Sripa B, Sirisinha S. Triptolide sensitizes resistant cholangiocarcinoma cells to TRAIL-induced apoptosis. Anticancer Res 2006; 26(1A): 259-65.
  10. Petvises S, Pakakasama S, Wongkajornsilp A, Sirireung S, Panthangkool W, Hongeng S. Ex vivo generation of cytokine-induced killer cells (CD3(+) CD56(+)) from post-stem cell transplant pediatric patients against autologous-Epstein-Barr virus-transformed lymphoblastoid cell lines. Pediatric transplantation. 2007 Aug;11(5):511-7.
  11. Maneechotesuwan K, Supawita S, Kasetsinsombat K, Wongkajornsilp A, Barnes PJ. Sputum indoleamine-2, 3-dioxygenase activity is increased in asthmatic airways by using inhaled corticosteroids. J Allergy Clin Immunol 2008;121(1):43-50.
  12. Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. J Allergy Clin Immunol 2009 Jan;123(1):239-48.
  13. Wongkajornsilp A, Somchitprasert T, Butraporn R, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, et al. Human cytokine-induced killer cells specifically infiltrated and retarded the growth of the inoculated human cholangiocarcinoma cells in SCID mice. Cancer Invest 2009 Feb;27(2):140-8.
  14. Panich U, Kongtaphan K, Onkoksoong T, Jaemsak K, Phadungrakwittaya R, Thaworn A, et al. Modulation of antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis. Cell Biol Toxicol 2010 Apr;26(2):103-16.
  15. Maneechotesuwan K, Wongkajornsilp A, Huabprasert S, Kasetsinsombat K, Suthumansami T. Differential expression of Th2 chemokine receptors on T cells from atopic and nonatopic asthmatics in response to Der p 1-pulsed dendritic cells. J Med Assoc Thailand 2010 Jan;93 Suppl 1:S62-70.
  16. Panich U, Kongtaphan K, Onkoksoong T, Jaemsak K, Phadungrakwittaya R, Thaworn A, et al. Modulation of antioxidant defense by Alpinia galanga and Curcuma aromatica extracts correlates with their inhibition of UVA-induced melanogenesis. Cell biol toxicol 2010;26(2):103-16.
  17. Maneechotesuwan K, Ekjiratrakul W, Kasetsinsombat K, Wongkajornsilp A, Barnes PJ. Statins enhance the anti-inflammatory effects of inhaled corticosteroids in asthmatic patients through increased induction of indoleamine 2, 3-dioxygenase. J allergy clin immunol 2010;126(4):754-62.
  18. Panich U, Tangsupa-a-nan V, Onkoksoong T, Kongtaphan K, Kasetsinsombat K, Akarasereenont P, et al. Inhibition of UVA-mediated melanogenesis by ascorbic acid through modulation of antioxidant defense and nitric oxide system. Arch Pharm Res 2011 May;34(5):811-20.
  19. Leelawat K, Narong S, Chaijan S, Sa-Ngiamsuntorn K, Disthabanchong S, Wongkajornsilp A, et al. Proteomic profiles of mesenchymal stem cells induced by a liver differentiation protocol. International journal of molecular sciences 2010;11(12):4905-15.
  20. Sa-ngiamsuntorn K, Wongkajornsilp A, Kasetsinsombat K, Duangsa-ard S, Nuntakarn L, Borwornpinyo S, et al. Upregulation of CYP 450s expression of immortalized hepatocyte-like cells derived from mesenchymal stem cells by enzyme inducers. BMC biotechnology 2011;11:89.
  21. Wongkajornsilp A, Sa-Ngiamsuntorn K, Hongeng S. Development of immortalized hepatocyte-like cells from hMSCs. Methods in molecular biology 2012;826:73-87.
  22. Panich U, Onkoksoong T, Limsaengurai S, Akarasereenont P, Wongkajornsilp A. UVA-induced melanogenesis and modulation of glutathione redox system in different melanoma cell lines: the protective effect of gallic acid. Journal of photochemistry and photobiology B, Biology 2012 Mar 1;108:16-22.
  23. Huabprasert S, Kasetsinsombat K, Kangsadalampai K, Wongkajornsilp A, Akarasereenont P, Panich U, et al. The Phyllanthus emblica L. infusion carries immunostimulatory activity in a mouse model. J Med Assoc Thai 2012 Feb;95 Suppl 2:S75-82.
  24. Phimolsarnti R, Charoenlap C, Ariyaboonsiri B, Wongkajornsilp A, Waikakul S. In vitro effects of thermoablation on apoptosis of giant cell tumor of bone: a preliminary report. J Med Assoc Thai. 2012;95 Suppl 9:S138-45.
  25. Maneechotesuwan K, Kasetsinsombat K, Wongkajornsilp A, Barnes PJ. Decreased indoleamine 2,3-dioxygenase activity and IL-10/IL-17A ratio in patients with COPD. Thorax. 2013;68:330-7.
  26. Maneechotesuwan K, Kasetsinsombat K, Wamanuttajinda V, Wongkajornsilp A, Barnes PJ. Statins enhance the effects of corticosteroids on the balance between regulatory T cells and Th17 cells. Clinical and experimental allergy 2013;43(2):212-22.
  27. Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-Ard S, Sa-Ngiamsuntorn K, Hongeng S, et al. Sunitinib Indirectly Enhanced Anti-Tumor Cytotoxicity of Cytokine-Induced Killer Cells and CD3+CD56+ Subset through the Co-Culturing Dendritic Cells. PloS one. 2013;8(11):e78980.
  28. Chotivichit A, Ruangchainikom M, Chiewvit P, Wongkajornsilp A, Sujirattanawimol K. Chronic spinal cord injury treated with transplanted autologous bone marrow-derived mesenchymal stem cells tracked by magnetic resonance imaging: a case report. J Med Case Rep. 2015;9(1):79.
  29. Maneechotesuwan K, Wongkajornsilp A, Adcock IM, Barnes PJ. Simvastatin Suppresses Airway IL-17 and Upregulates IL-10 in Patients With Stable COPD. Chest. 2015;148(5):1164-76.
  30. Pimolsanti R, Wongkajornsilp A, Chotiyarnwong P, Asavamongkolku A, Waikakul S. Effects of thermoablation with or without caffeine on giant cell tumour of bone. J Orthop Surg (Hong Kong). 2015;23(1):95-9.
  31. Maneechotesuwan K, Kasetsinsombat K, Wongkajornsilp A, Barnes PJ. Simvastatin up-regulates adenosine deaminase and suppresses osteopontin expression in COPD patients through an IL-13-dependent mechanism. Respir Res. 2016;17(1):104.
  32. Sa-Ngiamsuntorn K, Wongkajornsilp A, Phanthong P, Borwornpinyo S, Kitiyanant N, Chantratita W, et al. A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host. Virol J. 2016;13(1):59.
  33. Timalsena S, Pluangnooch P, Wongkajornsilp A, Soontrapa K. An additional CD28 costimulatory signal enhances proliferation and cytotoxicity of murine T cell-derived CIK cells. Asian Pac J Allergy Immunol. 2016.

Poster Presentations

  • Wongkajornsilp A, Sattawatthamrong Y, Huabprasert S, Poster number P.I.026, Primary cultures of cholangiocarcinoma established from bile fluid express insulin-like growth factor I and Fas, World Congress of Gastroenterology 2002, Diseases of the biliary tract, 12.30-13.30, Bitec Convention Center, Bangkok, Thailand
  • Wongkajornsilp A, Sattawatthamrong Y, Sangsuriyong S, Huabprasert S, Wamanutajinda V. The Partial Identification Of Immunosuppressor Derived From Cultured Cholangiocarcinoma. Abstract Book: XIVth World Congress of Pharmacology XIVth World Congress of Pharmacology, July 7 - 12, 2002, Moscone Convention Center, San Francisco, CA, USA.
  • Sangsuriyong S, Wongkajornsilp A, Huabprasert S, Wamanutajinda V. Suppression of insulin-like growth factor 1 or Fas ligand expression in a hamster cholangiocarcinoma cell line transplanted to allogeneic animals enhanced the immunogenicity against the tumor cells. Abstract Book: XIVth World Congress of Pharmacology, July 7 - 12, 2002, Moscone Convention Center, San Francisco, CA, USA.
  • Jamsak K, Tappayuththpijarn P, Ninchawee Ch., Huabprasert S, Thaworn A, Ananta W, Wongkajornsilp A, Ketsawatsakul U. Analysis of antioxidant phenolics in Thai Medicinal plants-modulateed cellular antioxidant enzymes. Abstract Book: XVth World Congress of Pharmacology, Beijing, China, July 2-7, 2006: p 336.
  • Boonmasawai S, Akarasereenont P, Tehchatrasak K, Wongkajornsilp A. The effect of COX inhibitors on endothelial cell proliferation activated by human cholangiocarcinoma cells. Abstract Book: XVth World Congress of Pharmacology, Beijing, China, July 2-7, 2006: p 291.
  • Kasetsinsombat K, Huabprasert S, Somchiprasert T, Wamanutajinda V, Maneechotesuwan K, Wongkajornsilp A. Swithching of Th1/Th2 expression profiles on CD4+ T cells after the incubation with dendritic cell pusled with mite extract allergens. Abstract Book: XVth World Congress of Pharmacology, Beijing, China, July 2-7, 2006: p 275.
  • Somchiprasert T, Sangsuriyong S, Wongkajornsilp A, Hongeng S, Waikakul S, Asavamongkolkul A, Huabprasert S. Effective osteosarcoma cytolysis using cytokine-induced killer cells pre-inoculated with tumor RNA-pulsed dendritic cells. Abstract Book: XVth Wold Congress of Pharmacology, Beijing, China, July 2-7, 2006: p 274.
  • Wongkajornsilp A, Vichchatorn P, Huabprasert S, Hongeng S. Dendritic cells pulsed with total tumor RNA enhanced cytokine-induced killer (CIK) cells-induced glioblastoma multiforme cytolysis. Abstract Book: XVth World Congress of Pharmacology, Beijing, China, July 2-7, 2006: p 274.
  • Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Hongeng S. Direct addition of picibanil to cytokine-induced killer cells improved the anti-cholangiocarcinoma cytotoxic activity. Abstract Book: 2010 Keystone Symposia on Molecular and Cellular Biology: Role of Inflammation in Oncogenesis and Molecular and Cellular Biology of Immune Escape in Cancer (J5/J6), Keystone, Colorado, USA, February 7-12, 2010: p 202.
  • Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Maneechotesuwan K. Suppression of CD274 expression in both mature and immature dendritic cells rendered greater anti-cholangiocarcinoma activity of the co-culturing cytokine-induced killer cells. Abstract book: Keystone Symposia on Molecular and Cellular Biology: Cancer control by tumor suppressors and immune effectors, February 12 - 17, 2011,Santa Fe, NM, p. 206.
  • Kasetsinsombat K, Wamanuttajinda V, Duangsa-ard S, Ariyaboonsiri B, Waikakul S, Maneechotesuwan K, Wongkajornsilp A. Addition of 1-methyl tryptophan to the co-culture of cytokine-induced killer cells with macrophages improved the anti-osteosarcoma cytolysis over those co-cultured with dendritic cells. Abstract book: Keystone Symposia on Molecular and Cellular Biology: Cancer control by tumor suppressors and immune effectors, February 12 - 17, 2011,Santa Fe, NM, p. 189.
  • Duangsa-ard S, Kasetsinsombat K, Wamanuttajinda V, Maneechotewuwan K, Wongkajornsilp A. Inhibition of indolamine 2,3-dioxygenase in dendritic cells improved anti-cholangiocarcinoma activity of cytokine-induced killer cells . Abstract book: Keystone Symposia on Molecular and Cellular Biology: Cancer control by tumor suppressors and immune effectors, February 12 - 17, 2011,Santa Fe, NM, p. 182.
  • Sa-ngiamsuntorn K, Wamanuttajinda V, Duangsa-ard S, Kasetsinsombat K, Maneechotesuwan K, Wongkajornsilp A. Treatment of CIK cells using Poly-G3 improved the anti-cholangiocarcinoma cytotoxicity. Abstract book: Keystone Symposia on Molecular and Cellular Biology: Cancer control by tumor suppressors and immune effectors, February 12 - 17, 2011, Santa Fe, NM, p. 199.
  • Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Maneechotesuwan K, Hongeng S, Sunitinib enhanced the anti-cholangiocarcinoma action of cytokine-induced killer cells through the action on CD3+CD56+AACR: Tumor Immunology: Multidisciplinary science driving basic and clinical advances, December 2 - 5, 2012, Miami, Florida, USA
  • Wongkajornsilp A, Wamanuttajinda V, Kasetsinsombat K, Duangsa-ard S, Maneechotesuwan K. Sunitinib-treated dendritic cells promoted Th1 phenotype in CD3+CD56+ subset of CIK cells. Keystone Symposia on Molecular and Cellular Biology: Cancer Immunology and Immunotherapy (J4), January 27, 2013 – February 1, 2013, Vancouver, British Columbia, Canada
  • Wongkajornsilp A, Wamanuttajinda V, Duangsa-ard S, Kasetsinsombat K, Maneechotesuwan K, Hongeng S. Clinical trial of neuroblastoma with cytokine-induced killer cells primed with sunitinib-pretreated dendritic cells. Keystone Symposia on Molecular and Cellular Biology: Immune Evolution in Cancer (X2), March 9 - 14, 2014, Whistler, British Columbia, Canada
  • Wongkajornsilp A, Bouma T, Duangsa-ard S, Kasetsinsombat K, Sawatpiboon N, Maneechotesuwan K, Hongeng S. Inhibition of TGF-β receptor kinase, but not the neutralization of TGF- β, improved the anti-tumor cytotoxic activity of cytokine-induced killer cells. Keystone Symposia on Molecular and Cellular Biology: Tumor Immunology: Multidisciplinary Science Driving Combination Therapy (J7), February 8 -13, 2015, Banff, Alberta, Canada