Printing tool Download PDF

Michael P. Myers

Group Leader

Work and Education History


Group Leader - Protein Networks

International Centre for Genetic Engineering and Biotechnology, Trieste, Italy


  • Established a successful proteomics core laboratory.
  • Developed software algorithms to intelligently reduce the search space in proteomic studies and for the efficient discovery of post-translational modifications.
  • Use of amine targeted mass tags for the structural characterization of Protein:RNA interactions.
  • Current research is focused on mapping post transtlational modification important in diseases such as cancer and diabetes.


Assistant Professor-Director of Proteomics

Cold Spring Harbor Laboratory, Cold Spring Harbor NY


  • Transformed the existing Protein Chemistry facility to a Mass Spectrometry based Proteomics core facility.
  • Developed an “on-bead” protocol for the rapid analysis of immune complexes including the mapping of binding epitopes and post translational modifications.
  • Developed the use of a variety of stable isotopes for the structural characterization of protein complexes.
  • Discovery and characterization of a novel ubiquitin-binding domain found in WRNIP1 and RAD18.
  • Pioneered the use of isotopically labeled peroxides to study the oxidation of Protein Tyrosine phosphatases by LC-MS.

Senior Research Fellow

Cold Spring Harbor Laboratory, Cold Spring Harbor NY


  • Characterized the substrate specificity of the PTP1B enzyme.
  • Identification of PTP1B as a negative regulator of the JAK-STAT pathway.


Postdoctoral Fellow

Cold Spring Harbor Laboratory, Cold Spring Harbor NY


  • Characterized the PTEN tumor suppressor protein, including the discovery that it antagonizes the PI 3-kinase pathway.


Ph.D. Student

Case Western Reserve University, Department of Neuroscience, Alzheimer Research Laboratory, Cleveland OH

Thesis Title:  Characterization of the dual specificity kinase CLK.


Undergraduate Student

DePauw University, Greencastle, IN

Degree:  B.A. in Biology/Physics

Invited Courses and Conferences


Advanced Bioinformatics for Systems Biology

ICGEB, Trieste, Italy

Course Organizer


Introductory PhD Course

ICGEB, Trieste, Italy

Introduction to DNA Replication and Repair


Translational Cancer Research, Cape Town, South Africa

ICGEB, Cape Town, South Africa

Protein Networks as a Tool for Understanding Cancer Biology


Advances in Prostate and Breast Cancer Research

ICGEB, Cape Town, South Africa

Protein Interaction and Systems Biology


Methods in Molecular and Systems Biology

Bilgi University, Istanbul, Turkey

Protein-Protein Interactions: from Primary Sequence to Large Scale Networks


University of Trieste Summer School

University of Trieste, Italy

Protein Interactions and Protein Networks


Genomic and Proteomic Approaches in Cancer Research

University of Cape Town, South Africa

Identification of Protein-Protein Interactions


The Genomics of Fish and its Pathogens

University of Santiago, Chile

Proteomic Analysis of Unsequenced Organisms


Translating Basic Research with Biomolecular Technology

Association of Biomolecular Research Facilities

How to Run a Facility


Introductory PhD Course

ICGEB, Trieste, Italy

Proteins: from Structures to Networks


Protein Purification Course

Cold Spring Harbor Laboratory, New York

Analysis of Proteins and Peptides by Mass Spectrometry


Integrating Science, Tools and Technology with Systems Biology

Association of Biomolecular Research Facilities

Adaptation of MALDI-TOF Mass Spectrometry for the Analysis of Phosphopeptides


New England Mass Spectrometry Users Meeting

Thermo Finnigan Corporation

Rapid Analysis of Protein-Protein Interactions by Mass Spectrometry


Special Topics Course: Protein Networks

Cold Spring Harbor Laboratory, New York

Course Organizer


Scientific Reasoning and Logic:  Fundamentals of Protein Analysis

Cold Spring Harbor Laboratory, New York

Course Organizer


Proteomics Course

Cold Spring Harbor Laboratory, New York

Fundamentals of MALDI-TOF/TOF Mass Spectrometry


67. Cesaratto, F., Sasset, L., Myers, M.P., Re, A., Petris, G., and Burrone, O.R. (2019). BiP/GRP78 Mediates ERAD Targeting of Proteins Produced by Membrane-Bound Ribosomes Stalled at the STOP-Codon. J. Mol. Biol. 431, 123–141. 

 66. Collesi, C., Felician, G., Secco, I., Gutierrez, M.I., Martelletti, E., Ali, H., Zentilin, L., Myers, M.P., and Giacca, M. (2018). Reversible Notch1 acetylation tunes proliferative signalling in cardiomyocytes. Cardiovasc. Res. 114, 103–122. 

 65. Durigutto, P., Grossi, C., Borghi, M.O., Macor, P., Pregnolato, F., Raschi, E., Myers, M.P., de Groot, P.G., Meroni, P.L., and Tedesco, F. (2018). New insight into antiphospholipid syndrome: antibodies to β2glycoprotein I-domain 5 fail to induce thrombi in rats. Haematologica haematol. 2018.198119. 

64. Samec, N., Jovcevska, I., Stojan, J., Zottel, A., Liovic, M., Myers, M.P., Muyldermans, S., Šribar, J., Križaj, I., and Komel, R. (2018). Glioblastoma-specific anti-TUFM nanobody for in-vitro immunoimaging and cancer stem cell targeting. Oncotarget 9, 17282–17299. 

63. Di Stazio, M., Collesi, C., Vozzi, D., Liu, W., Myers, M., Morgan, A., D′Adamo, P.A., Girotto, G., Rubinato, E., Giacca, M., et al. (2018). TBL1Y: a new gene involved in syndromic hearing loss. Eur. J. Hum. Genet. 

62. Jovčevska, I., Zupanec, N., Urlep, Ž., Vranič, A., Matos, B., Stokin, C.L., Muyldermans, S., Myers, M.P., Buzdin, A.A., Petrov, I., et al. (2017). Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget 8, 44141–44158. 

 61. Marcassa, E., Raimondi, M., Anwar, T., Eskelinen, E.-L., Myers, M.P., Triolo, G., Schneider, C., and Demarchi, F. (2017). Calpain mobilizes Atg9/Bif-1 vesicles from Golgi stacks upon autophagy induction by thapsigargin. Biol. Open 6, 551–562. 

60. Allemand, E., Myers, M.P., Garcia-Bernardo, J., Harel-Bellan, A., Krainer, A.R. and Muchardt, C. (2016) A Broad Set of Chromatin Factors Influences Splicing. PLoS Genet. 12(9): e1006318.

59. Thomas, M., Myers, M.P., Massimi, P., Guarnaccia, C. and Banks, L. (2016) Analysis of Multiple HPV E6 PDZ Interactions Defines Type-Specific PDZ Fingerprints that Predict Oncogenic Potential. PLoS Path. 12(8): e1005766.

58. Subbaiah, V.K., Zhang, Y., Rajagopalan, D., Abdullah, L.N., Yeo-Teh, N.S.L., Tomaic, V., Banks, L., Myers, M.P., Chow, E.K., and Jha, S. (2015). E3 ligase EDD1/UBR5 is utilized by the HPV E6 oncogene to destabilize tumor suppressor TIP60. Oncogene 35, 2062-74. 

57. Jovčevska, I., Zupanec, N., Kočevar, N., Cesselli, D., Podergajs, N., Stokin, C.L., Myers, M.P., Muyldermans, S., Ghassabeh, G.H., Motaln, H., et al. (2014). TRIM28 and b-Actin Identified via Nanobody-Based Reverse Proteomics Approach as Possible Human Glioblastoma Biomarkers. PLoS One 9, e113688.

56. Kertész-Farkas, A., Reiz, B., Vera, R., Myers, M.P., and Pongor, S. (2014). PTMTreeSearch: a novel two-stage tree-search algorithm with pruning rules for the identification of post-translational modification of proteins in MS/MS spectra. Bioinformatics 30, 234–241.

55. Reiz, B., P Myers, M., Pongor, S., and Kertesz-Farkas, A. (2014). Precursor mass dependent filtering of mass spectra for proteomics analysis. Protein Pept. Lett. 21, 858–863.

54. Todorovic, B., Nichols, A.C., Chitilian, J.M., Myers, M.P., Shepherd, T.G., Parsons, S.J., Barrett, J.W., Banks, L., and Mymryk, J.S. (2014). The Human Papillomavirus E7 Proteins Associate with p190RhoGAP and Alter Its Function. J. Virol. 88, 3653–3663.

53. Zecchin, A., Pattarini, L., Gutierrez, M.I., Mano, M., Mai, A., Valente, S., Myers, M.P., Pantano, S., and Giacca, M. (2014). Reversible acetylation regulates vascular endothelial growth factor receptor-2 activity. J. Mol. Cell Biol. 6, 116–127.

52. Bhardwaj, A., Myers, M.P., Buratti, E., and Baralle, F.E. (2013). Characterizing TDP-43 interaction with its RNA targets. Nucleic Acids Res. 41, 5062–5074.

51. Cataldo, F., Peche, L.Y., Klaric, E., Brancolini, C., Myers, M.P., Demarchi, F., and Schneider, C. (2013). CAPNS1 Regulates USP1 Stability and Maintenance of Genome Integrity. Mol. Cell. Biol. 33, 2485–2496.

50. González, J.F., Myers, M.P., and Venturi, V. (2013). The inter‐kingdom solo OryR regulator of Xanthomonas oryzae is important for motility. Mol. Plant Pathol. 14, 211–221.

49. Reiz, B., Kertész-Farkas, A., Pongor, S., and Myers, M.P. (2013). Chemical rule-based filtering of MS/MS spectra. Bioinformatics 29, 925–932.

48. Thomas, M., Tomaić, V., Pim, D., Myers, M.P., Tommasino, M., and Banks, L. (2013). Interactions between E6AP and E6 proteins from alpha and beta HPV types. Virology 435, 357–362.

47. Berić, T., Kojić, M., Stanković, S., Topisirović, L., Degrassi, G., Myers, M., Venturi, V., and Fira, D. (2012). Antimicrobial activity of Bacillus sp. natural isolates and their potential use in the biocontrol of phytopathogenic bacteria. Food Technol. Biotechnol. 50, 25–31

46. González, J.F., Degrassi, G., Devescovi, G., De Vleesschauwer, D., Höfte, M., Myers, M.P., and Venturi, V. (2012). A proteomic study of Xanthomonas oryzae pv. oryzae in rice xylem sap. J. Proteomics 75, 5911–5919.

45. Kertész-Farkas, A., Reiz, B., P Myers, M., and Pongor, S. (2012). Database searching in mass spectrometry based proteomics. Curr. Bioinform. 7, 221–230.

44. Reiz, B., Kertész-Farkas, A., Pongor, S., and P Myers, M. (2012). Data preprocessing and filtering in mass spectrometry based proteomics. Curr. Bioinform. 7, 212–220.

43. Subbaiah, V.K., Massimi, P., Boon, S.S., Myers, M.P., Sharek, L., Garcia-Mata, R., and Banks, L. (2012). The invasive capacity of HPV transformed cells requires the hDlg-dependent enhancement of SGEF/RhoG activity. PLoS Pathog. 8, e1002543.

42. Zhang, X.C., Piccini, A., Myers, M.P., Van Aelst, L., and Tonks, N.K. (2012). Functional analysis of the protein phosphatase activity of PTEN. Biochem. J. 444, 457–464.

41. Hoek, M., Myers, M.P., and Stillman, B. (2011). An analysis of CAF-1-interacting proteins reveals dynamic and direct interactions with the KU complex and 14-3-3 proteins. J. Biol. Chem. 286, 10876–10887.

40. Kertész-Farkas, A., Reiz, B., Myers, M.P., and Pongor, S. (2011). PTMSearch: a greedy tree traversal algorithm for finding protein post-translational modifications in tandem mass spectra. Mach. Learn. Knowl. Discov. Databases 162–176.

39. Kula, A., Guerra, J., Knezevich, A., Kleva, D., Myers, M.P., and Marcello, A. (2011). Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function. Retrovirology 8, 60.

38. Tomaić, V., Pim, D., Thomas, M., Massimi, P., Myers, M.P., and Banks, L. (2011). Regulation of the human papillomavirus type 18 E6/E6AP ubiquitin ligase complex by the HECT domain-containing protein EDD. J. Virol. 85, 3120–3127.

37. Hinton, S., Myers, M., Roggero, V., Allison, L., and Tonks, N. (2010). The pseudophosphatase MK-STYX interacts with G3BP and decreases stress granule formation. Biochem. J. 427, 349–357.

36. Mangone, M., Myers, M.P., and Herr, W. (2010). Role of the HCF-1 basic region in sustaining cell proliferation. PLoS One 5, e9020.

35. Nagasaka, K., Pim, D., Massimi, P., Thomas, M., Tomaić, V., Subbaiah, V.K., Kranjec, C., Nakagawa, S., Yano, T., and Taketani, Y. (2010). The cell polarity regulator hScrib controls ERK activation through a KIM site-dependent interaction. Oncogene 29, 5311–5321.

34. Sinha, R., Allemand, E., Zhang, Z., Karni, R., Myers, M.P., and Krainer, A.R. (2010). Arginine methylation controls the subcellular localization and functions of the oncoprotein splicing factor SF2/ASF. Mol. Cell. Biol. 30, 2762–2774.

33. Tomaić, V., Gardiol, D., Massimi, P., Ozbun, M., Myers, M., and Banks, L. (2009). Human and primate tumour viruses use PDZ binding as an evolutionarily conserved mechanism of targeting cell polarity regulators. Oncogene 28, 1–8.

32. Bish, R.A., Fregoso, O.I., Piccini, A., and Myers, M.P. (2008). Conjugation of complex polyubiquitin chains to WRNIP1. J. Proteome Res. 7, 3481–3489.

31. Allemand, E., Hastings, M.L., Murray, M. V, Myers, M.P., and Krainer, A.R. (2007). Alternative splicing regulation by interaction of phosphatase PP2Cγ with nucleic acid binding protein YB-1. Nat. Struct. Mol. Biol. 14, 630–638. 

30. Bish, R.A., and Myers, M.P. (2007). Werner helicase-interacting protein 1 binds polyubiquitin via its zinc finger domain. J. Biol. Chem. 282, 23184–23193.

29. Hastings, M.L., Allemand, E., Duelli, D.M., Myers, M.P., and Krainer, A.R. (2007). Control of pre-mRNA splicing by the general splicing factors PUF60 and U2AF 65. PLoS One 2, e538.

28. Huang, Y., Myers, M.P., and Xu, R.-M. (2006). Crystal structure of the HP1-EMSY complex reveals an unusual mode of HP1 binding. Structure 14, 703–712.

27. Li, Z., Cao, R., Wang, M., Myers, M.P., Zhang, Y., and Xu, R.-M. (2006). Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex. J. Biol. Chem. 281, 20643–20649.

26. Narita, M.M., Narita, M.M., Krizhanovsky, V., Nuñez, S., Chicas, A., Hearn, S.A., Myers, M.P., and Lowe, S.W. (2006). A novel role for high-mobility group a proteins in cellular senescence and heterochromatin formation. Cell 126, 503–514.

25. Pegoraro, G., Marcello, A., Myers, M.P., and Giacca, M. (2006). Regulation of adeno-associated virus DNA replication by the cellular TAF-I/set complex. J. Virol. 80, 6855–6864.

24. Allemand, E., Guil, S., Myers, M., Moscat, J., Cáceres, J.F., and Krainer, A.R. (2005). Regulation of heterogenous nuclear ribonucleoprotein A1 transport by phosphorylation in cells stressed by osmotic shock. Proc. Natl. Acad. Sci. U. S. A. 102, 3605–3610.

23. Duelli, D.M., Hearn, S., Myers, M.P., and Lazebnik, Y. (2005). A primate virus generates transformed human cells by fusion. J. Cell Biol. 171, 493–503. 

22. Pegoraro, G., Marcello, A., Myers, M.P., and Giacca, M. (2005). Human ANP32B Protein Binds AAV-2 Rep and Increases Viral Replication and rAAV Production. Mol. Ther. 11, S331–S331.

21. Janardhan, A., Swigut, T., Hill, B., Myers, M.P., and Skowronski, J. (2004). HIV-1 Nef binds the DOCK2–ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis. PLoS Biol. 2, e6.

20. Caudy, A.A., Ketting, R.F., Hammond, S.M., Denli, A.M., Bathoorn, A.M.P., Tops, B.B.J., Silva, J.M., Myers, M.M., Hannon, G.J., and Plasterk, R.H.A. (2003). A micrococcal nuclease homologue in RNAi effector complexes. Nature 425, 411–414.

19. Ono, T., Losada, A., Hirano, M., Myers, M.P., Neuwald, A.F., and Hirano, T. (2003). Differential contributions of condensin I and condensin II to mitotic chromosome architecture in vertebrate cells. Cell 115, 109–121.

18. Salmeen, A., Andersen, J.N., Myers, M.P., Meng, T.-C., Hinks, J.A., Tonks, N.K., and Barford, D. (2003). Redox regulation of protein tyrosine phosphatase 1B involves a sulphenyl-amide intermediate. Nature 423, 769–773.

17. Torres, J., Rodriguez, J., Myers, M.P., Valiente, M., Graves, J.D., Tonks, N.K., and Pulido, R. (2003). Phosphorylation-regulated Cleavage of the Tumor Suppressor PTEN by Caspase-3. J. Biol. Chem. 278, 30652–30660.

16. Wysocka, J., Myers, M.P., Laherty, C.D., Eisenman, R.N., and Herr, W. (2003). Human Sin3 deacetylase and trithorax-related Set1/Ash2 histone H3-K4 methyltransferase are tethered together selectively by the cell-proliferation factor HCF-1. Genes Dev. 17, 896–911.

15. Caudy, A.A., Myers, M., Hannon, G.J., and Hammond, S.M. (2002). Fragile X-related protein and VIG associate with the RNA interference machinery. Genes Dev. 16, 2491–2496.

14. Hu, P., Wu, S., Sun, Y., Yuan, C.-C., Kobayashi, R., Myers, M.P., and Hernandez, N. (2002). Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. Mol. Cell. Biol. 22, 8044–8055.

13. Myers, M.P., Andersen, J.N., Cheng, A., Tremblay, M.L., Horvath, C.M., Parisien, J.-P., Salmeen, A., Barford, D., and Tonks, N.K. (2001). TYK2 and JAK2 are substrates of protein-tyrosine phosphatase 1B. J. Biol. Chem. 276, 47771–47774.

12. Wen, S., Stolarov, J., Myers, M.P., Su, J.D., Wigler, M.H., Tonks, N.K., and Durden, D.L. (2001). PTEN controls tumor-induced angiogenesis. Proc. Natl. Acad. Sci. U. S. A. 98, 4622–4627.

11. Menegay, H.J., Myers, M.P., Moeslein, F.M., and Landreth, G.E. (2000). Biochemical characterization and localization of the dual specificity kinase CLK1. J. Cell Sci. 113, 3241–3253 

10. Salmeen, A., Andersen, J.N., Myers, M.P., Tonks, N.K., and Barford, D. (2000). Molecular basis for the dephosphorylation of the activation segment of the insulin receptor by protein tyrosine phosphatase 1B. Mol. Cell 6, 1401–1412.

9. Moeslein, F.M., Myers, M.P., and Landreth, G.E. (1999). The CLK family kinases, CLK1 and CLK2, phosphorylate and activate the tyrosine phosphatase, PTP-1B. J. Biol. Chem. 274, 26697–26704.

8. Tonks, N.K., and Myers, M.P. (1999). Structural assets of a tumor suppressor. Science (80-. ). 286, 2096–2097.

7. Li, J., Simpson, L., Takahashi, M., Miliaresis, C., Myers, M.P., TONKS, N., and Parsons, R. (1998). The PTEN/MMAC1 tumor suppressor induces cell death that is rescued by the AKT/protein kinase B oncogene. Cancer Res. 58, 5667–5672.

6. Myers, M.P., Pass, I., Batty, I.H., Van der Kaay, J., Stolarov, J.P., Hemmings, B.A., Wigler, M.H., Downes, C.P., and Tonks, N.K. (1998). The lipid phosphatase activity of PTEN is critical for its tumor supressor function. Proc. Natl. Acad. Sci. U. S. A. 95, 13513–13518.

5. Myers, M.P., and Tonks, N.K. (1997). PTEN: sometimes taking it off can be better than putting it on. Am. J. Hum. Genet. 61, 1234.

4. Myers, M.P., Stolarov, J.P., Eng, C., Li, J., Wang, S.I., Wigler, M.H., Parsons, R., and Tonks, N.K. (1997). P-TEN, the tumor suppressor from human chromosome 10q23, is a dual-specificity phosphatase. Proc. Natl. Acad. Sci. U. S. A. 94, 9052–9057.

3. Myers, M.P., Swanson, K.D., and Landreth, G. (1996). Mechanisms of growth factor-mediated signal transduction in PC12 cells. Genet. Mech. Mult. Endocr. Neoplasia Type 2 61–98.

2. Myers, M.P., Murphy, M.B., and Landreth, G. (1994). The dual-specificity CLK kinase induces neuronal differentiation of PC12 cells. Mol. Cell. Biol. 14, 6954–6961.

1. Callaghan, T., Antczak, M., Flickinger, T., Raines, M., Myers, M., and Kung, H.-J. (1993). A complete description of the EGF-receptor exon structure: implication in oncogenic activation and domain evolution. Oncogene 8, 2939–2948.

Institutional Support

Grant Review Committees

2004              National Institute of Aging, Special study section on Proteomics in Aging, Washington D.C.

2005              National Institute of Health, Shared Instrumentation study section, Washington D.C.


Internal Committees

2017-present      ICGEB:  Meetings and Courses Committee.

2016-present      ICGEB: Collaborative Research Program Committee.

2014-present      ICGEB: Fellowship Committee.

2001-2004           Watson School of Biological Science, CSHL, New York: Admissions Committee.

2002-2003           Watson School of Biological Science, CSHL, New York:  URP Admissions Committee.

References Available Upon Request